Disposable biosensor based on cathodic electrochemiluminescence of tris(2,2-bipyridine)ruthenium(II) for uric acid determination

Ballesta-Claver, J.; Rodríguez-Gómez, R.; Capitán-Vallvey, L.F.

doi:10.1016/j.aca.2013.01.045

Cited by 41 publications

(22 citation statements)

References 33 publications

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“…In the case of urine samples, the wide range of pH values found in samples can be challenging [ 14 ]. In addition to pH adjustment, centrifugation and dilution are two of the most often used sample preparation techniques as described for analytes, such as pirazinamide [ 58 ], anti-malarial drug (Artesunate) [ 65 ], testosterone [ 66 ], homocysteine [ 67 ], nuclear matrix protein 22 [ 68 ], dopamine [ 69 ], and uric acid [ 70 – 72 ].…”

Section: Pairing (Electrochemical) Biosensors With Sample Preparationmentioning

confidence: 99%

Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples

Bunyakul

Baeumner

2014

Sensors

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Clinical analyses benefit world-wide from rapid and reliable diagnostics tests. New tests are sought with greatest demand not only for new analytes, but also to reduce costs, complexity and lengthy analysis times of current techniques. Among the myriad of possibilities available today to develop new test systems, amperometric biosensors are prominent players—best represented by the ubiquitous amperometric-based glucose sensors. Electrochemical approaches in general require little and often enough only simple hardware components, are rugged and yet provide low limits of detection. They thus offer many of the desirable attributes for point-of-care/point-of-need tests. This review focuses on investigating the important integration of sample preparation with (primarily electrochemical) biosensors. Sample clean up requirements, miniaturized sample preparation strategies, and their potential integration with sensors will be discussed, focusing on clinical sample analyses.

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Section: Pairing (Electrochemical) Biosensors With Sample Preparationmentioning

confidence: 99%

Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples

Bunyakul

Baeumner

2014

Sensors

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“…It is important to regulate the amount uric acid in maintaining the health of the bloodstream, since low amount of uric acid may lead to atherosclerosis and stroke [2]. Uric acid can be monitored using two methods; blood test measurement [3-6] and urine test measurement [1,[7][8][9][10][11][12][13][14][15]. A healthy range for uric acid levels in blood serum is from 3.5 to 7.2 mg/dL in men and from 2.6 to 6 mg/dL for women [14].…”

Section: Introductionmentioning

confidence: 99%

Uric acid detection in visible spectrum

et al. 2020

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The measurement of uric acid based on the optical absorption at visible light spectrum is investigated and tested. Sensing in the visible region was conducted for determination of suitable wavelength that produces high sensitivity and accuracy performance based on the Beer-Lambert law calculation. In this work, the uric acid is detected by detecting sodium urate as a product of chemical reaction between uric acid with sodium hydroxide buffer. The setup has been tested for uric acid concentration ranging from 15 mg/dL to 85 mg/dL. Three wavelengths have been analyzed which are 460 nm, 525 nm and 630 nm. Measured data at 460nm wavelength exhibits the highest sensitivity, which is 0.0012 (mg/dL)-with 86.51% accuracy. Detection of uric acid at visible light spectrum offers a low-cost sensor based on visible LEDs and photodiode is possible to be realized.

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“…The ECL sensor has extensive advantages, such as high sensitivity, wide detection range, good controllability, fast response and strong anti-interference ability and so on, which make it especially suitable for the detection of trace and ultra-trace amount of complex substances [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. However, traditional ECL detection equipment based on magnetic separation technology needs to integrate some necessary components including discrete three-electrode system (working electrode, counter electrode and reference electrode), ultra weak photoelectric detection system, focusing lens system, magnetic separation and enrichment system and micro channel sampling and cleaning system into a unified ECL detection pool, which brings it some defects of high price, bulk and weight, inconvenient cleaning and so on.…”

Section: Introductionmentioning

confidence: 99%

Detection of Abrin by Electrochemiluminescence Biosensor Based on Screen Printed Electrode

Liu¹,

Tong²,

Mu³

et al. 2018

Sensors

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For the convenience of fast measurement in the outdoor environment, a portable electrochemiluminescence biosensor with the screen-printed electrode as the reaction center was developed, which possesses the characteristics of high sensitivity, small scale, simplified operation and so on, and has been used for in situ detection of abrin. First, combining with magnetic separation technique, the "biotin-avidin" method was used to immobilize the polyclonal antibody (pcAb) on the magnetic microspheres surface as the capture probe. Secondly, the Ru(bpy) 3 2+ -labeled monoclonal antibody (mcAb) was used as the specific electrochemiluminescence signal probe. Then, the "mcAb-toxin-pcAb" sandwich model was built to actualize the quantitative detection of abrin on the surface of the screen-printed electrode. The linear detection range was 0.5-1000 ng/mL; the regression equation was Y = 89.251lgX + 104.978 (R = 0.9989, n = 7, p < 0.0001); and the limit of detection (LOD) was 0.1 ng/mL. The sensing system showed high sensitivity, excellent specificity and good anti-interference ability, and could be used for the analysis of trace abrin in various environmental samples with good recovery and reproducibility. Compared with the traditional electrochemiluminescence sensing device, its miniaturization and portability gives it potential to satisfy the requirement of in situ detection.

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Disposable biosensor based on cathodic electrochemiluminescence of tris(2,2-bipyridine)ruthenium(II) for uric acid determination

Cited by 41 publications

References 33 publications

Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples

Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples

Uric acid detection in visible spectrum

Detection of Abrin by Electrochemiluminescence Biosensor Based on Screen Printed Electrode

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