Disposable Electrochemical Aptasensor for Ultrasensitive Determination of Aflatoxin B1 Using Copper Nanoparticles as Probes

Abstract

An electrochemical aptasensor for aflatoxin B1 (AFB1) detection was constructed based on the copper nanoparticles (CuNPs) and gold nanoflowers modified screen-printed carbon electrodes as electrochemical probes and substrates, respectively. In the range of 100 aM to 100 pM, a good linear relationship between oxidation peak current of CuNPs and concentration of AFB1 was obtained. The high sensitivity could be ascribed to the amplified electrochemical signals by CuNPs. The high affinity of AFB1 with aptamer endowed its high selectivity. The above advantages and disposable traits made this aptasensor as an ideal platform for evaluation of AFB1 level in food samples.

Recent Progress of Electrochemical Aptasensors toward AFB1 Detection (2018–2023)

“…Multiple label-free electrochemical aptasensors based on carbon nanotubes [82,87], graphene oxide [80], reduced graphene oxide [55,77,82,85,87], AuNPs [42,76,77,87] or Aubased materials [51,83,86,90], polymers [49,77,80], and MOFs [79] have been reported recently.…”

A label-free aptasensor based on electrodeposition of gold nanoparticles on silver-based metal–organic frameworks for measuring ochratoxin A in black and red pepper

“…Aptamers, which are synthetic single-stranded oligonucleotides, are increasingly used as replacements for antibodies in the development of biosensors due to their strong binding affinity, high selectivity, excellent thermal stability, screened and chemical synthesis in vitro, short preparation time, and low cost. 17,18 They can also effectively bind to a wide range of targets. These properties make aptamers a popular choice for biosensor applications.…”