Disposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samples

Baldo, Thaísa A.; Proença, Camila dos Anjos; Felix, Fabiana da Silva; Freitas, Tayane A.; Sakata, Solange K.; Angnes, Lúcio; Faria, Ronaldo C.

doi:10.1016/j.talanta.2021.122447

Cited by 20 publications

(15 citation statements)

References 28 publications

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“…Their immunoplatform involves the selective capture of sandwich antibody-target analyte-HRP labelled detector antibody sandwich onto a carboxylic acid-functionalised magnetic bead (HOOC-MBs), as shown in Figure 5B, exhibiting a LOD of 0.1 nm/mL for the determination of OM in unprocessed eggs and flour and baked bread [51]. Another example targeting egg allergen (ovalbumin, OVA), this time in wine samples, has been reported by Baldo et al [52]. In the wine industry, egg white can be utilised as a fining agent to assist in the removal of tannins.…”

Section: Allergen Examplesmentioning

confidence: 93%

Electroanalytical Overview: Electrochemical Sensing Platforms for Food and Drink Safety

2021

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Robust, reliable, and affordable analytical techniques are essential for screening and monitoring food and water safety from contaminants, pathogens, and allergens that might be harmful upon consumption. Recent advances in decentralised, miniaturised, and rapid tests for health and environmental monitoring can provide an alternative solution to the classic laboratory-based analytical techniques currently utilised. Electrochemical biosensors offer a promising option as portable sensing platforms to expedite the transition from laboratory benchtop to on-site analysis. A plethora of electroanalytical sensor platforms have been produced for the detection of small molecules, proteins, and microorganisms vital to ensuring food and drink safety. These utilise various recognition systems, from direct electrochemical redox processes to biological recognition elements such as antibodies, enzymes, and aptamers; however, further exploration needs to be carried out, with many systems requiring validation against standard benchtop laboratory-based techniques to offer increased confidence in the sensing platforms. This short review demonstrates that electroanalytical biosensors already offer a sensitive, fast, and low-cost sensor platform for food and drink safety monitoring. With continued research into the development of these sensors, increased confidence in the safety of food and drink products for manufacturers, policy makers, and end users will result.

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Section: Allergen Examplesmentioning

confidence: 93%

Electroanalytical Overview: Electrochemical Sensing Platforms for Food and Drink Safety

2021

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“…A disposable electrochemical microfluidic device (DEµD) based on a sandwich immunosensing platform was developed for the detection of OVA in wine samples [89]. In fact, the sensing platform involves the sandwiching of OVA including OVA-polyclonal antibody and HRP-labeled OVA polyclonal antibody immobilized on MBs, as reported for OM [82].…”

Section: Immunosensorsmentioning

confidence: 99%

“…The immunocomplex between OVA and OVA-HRP-Ab-MBs antibody, produced by the immunoreaction of the analyte and the HPRP-labeled antibody, was injected in DEµD where the immune-sandwich on 8-WEs was generated and the electrochemical response for OVA detection was investigated by amperometry. More details on the injection system and the corresponding analytical procedure are available in [89]. Under optimized experimental conditions, a linear concentrations range of 0.01-10 pg mL −1 and an LOD of 0.2 fg mL −1 were obtained.…”

Section: Immunosensorsmentioning

confidence: 99%

“…Another factor to consider is the possibility of using portable user-friendly devices that can allow analysis, for example, at the restaurant or at home [13,107]. In this framework, the integrated exogenous antigen testing (iEAT) is a very interesting example of a user-friendly and simple smartphone-based electrochemical food analyzer based on a sandwich immunomagnetic assay format [108] in analogy with the examples of immunosensors reported above [73,74,82,89,97]. The target proteins were gliadin in wheat, Ara h 1 in peanut, Cor a 1 in hazelnut, casein in milk, and OVA in egg white.…”

Section: Immunosensorsmentioning

confidence: 99%

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Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Curulli

2022

Biosensors

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Food allergy has been indicated as the most frequent adverse reaction to food ingredients over the past few years. Since the only way to avoid the occurrence of allergic phenomena is to eliminate allergenic foods, it is essential to have complete and accurate information on the components of foodstuff. In this framework, it is mandatory and crucial to provide fast, cost-effective, affordable, and reliable analysis methods for the screening of specific allergen content in food products. This review reports the research advancements concerning food allergen detection, involving electrochemical biosensors. It focuses on the sensing strategies evidencing different types of recognition elements such as antibodies, nucleic acids, and cells, among others, the nanomaterial role, the several electrochemical techniques involved and last, but not least, the ad hoc electrodic surface modification approaches. Moreover, a selection of the most recent electrochemical sensors for allergen detection are reported and critically analyzed in terms of the sensors’ analytical performances. Finally, advantages, limitations, and potentialities for practical applications of electrochemical biosensors for allergens are discussed.

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“…The main allergic reactions to food are attributed only to a limited variety of proteins, which are considered the main allergens ( 3 ). For example, ovalbumin (OVA) accounts for half of egg white protein and is the main allergen of eggs, which often causes allergic reactions especially in children ( 4 , 5 ). Although various approaches have been applied to control food allergies, the most efficient way is to avoid intaking allergens currently.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a 3-Dimensional Intestinal Cell Model to Simulate the Intestinal Mucosal Immune System for Food Allergy Investigations

Lin

Wang

et al. 2022

Front. Immunol.

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Food allergy is a worldwide food safety problem with increasing prevalence. Developing novel approaches for food allergy investigations is the basis for controlling food allergies. In this work, a 3-dimensional (3D) intestinal cell model was established to simulate the intestinal mucosal immune system. Gut epithelial cell line CMT93 was cultured in a transwell insert above dendritic cells (DCs) isolated from mouse spleen and stimulated by egg allergen ovalbumin (OVA), then the conditioned media of DCs was transferred to T cells isolated from mouse spleen. The allergy-related indexes of each cell type were determined by qPCR and flow cytometry. Then the TAZ gene was knocked down in the CMT93 cells and the role of the Hippo pathway in OVA-induced food allergy was investigated. The 3D intestinal cell model showed more significant and more specific allergic responses than conventional cell models and is more convenient to be manipulated than the mouse models. This model is an ideal tool for food allergy investigations and would facilitate studies in the field of intestinal mucosal immunity.

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Disposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samples

Cited by 20 publications

References 28 publications

Electroanalytical Overview: Electrochemical Sensing Platforms for Food and Drink Safety

Electroanalytical Overview: Electrochemical Sensing Platforms for Food and Drink Safety

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Establishment of a 3-Dimensional Intestinal Cell Model to Simulate the Intestinal Mucosal Immune System for Food Allergy Investigations

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