Disposable Immunochips for the Detection of Legionella pneumophila Using Electrochemical Impedance Spectroscopy

Abstract

The rapid diagnosis of Legionellosis is crucial for the effective treatment of this disease. Currently, most clinical laboratories utilize rapid immunoassays that are sufficient for the detection of Legionella serogroup 1, but not other clinically relevant serogroups. In this report, the development of a disposable immunochip system is described in connection with electrochemical impedance spectroscopy and fluorescence microscopy. The immunochips were prepared by covalently immobilizing fluorophore-conjugated L. pneumophilaantibodies on Au chips. The analytical performance of the immunochips was optimized as a prescreening tool for L. pneumophila. The versatile immunochips described here can be easily adapted for the monitoring of all Legionella serogroups in clinical and environmental samples.

Electrochemical Detection of Amyloid-Beta Aggregation in the Presence of Resveratrol

“…[36][37][38][39] Previously, voltammetric techniques have been employed to study the antioxidant ability of resveratrol. Corduneanu and coworkers 40 reported that, the antioxidant activity of resveratrol was dependent on the pH of the supporting electrolyte and was shown to be a moderate strength antioxidant with irreversible oxidation processes.…”

Rapid Legionella pneumophila determination based on a disposable core–shell Fe 3 O 4 @poly(dopamine) magnetic nanoparticles immunoplatform

“…Other conventional detection tests use direct immunofluorescence assay, urinary antigen test, serology testing or polymerase chain reaction [8]. However, these methods have turnaround times measurable in several hours to days, are expensive, technically demanding, and limited in their detection of different serogroups [2]. Therefore, sensitive and rapid detection methods for the sensitive and selective detection of this target bacterium in water systems are of great interest.…”

“…Electrochemical immunosensors have attracted great interest because they combine the high selectivity provided by immunological species with the high sensitivity achieved with modern electrochemical techniques [2,[5][6][7] and have the advantages of rapid analysis time, ability of detecting analytes in complex biological media with high specificity and sensitivity and a relatively inexpensive cost compared to other immunoassay methods. In particular, two M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 5 electrochemical immunosensors have been reported for L. pneumophila determination [2].…”

“…In particular, two M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 5 electrochemical immunosensors have been reported for L. pneumophila determination [2].…”

“…Epidemiological data indicates that L. pneumophila is responsible for 91.5% of diagnosed Legionellosis cases with serogroup 1 (SG1) being the predominant serotype (84.2%) [2,3]. Although infection with non-pneumophila Legionella species (Legionella longbeachae and Legionella micdadei) can occur, its virulence is much lower.…”