Disposable pipette tips extraction: Fundamentals, applications and state of the art

Abstract

Disposable pipette tips extraction consists of a solid-phase extraction in which the sorbent is poorly dispersed in a pipette tip, which allows a quick and dynamic contact between the aspirated analyte from the sample and the solid phase. It is a technique used particularly in food and forensic analysis, since it requires a small amount of sample and solvent. This article highlights the principles, advantages and disadvantages of the disposable pipette tips extraction method and reviews recent applications.

Assessment of a Fully Optimized DPX-Based Procedure for the Multiclass Determination of Pesticides in Drinking Water Using High-Performance Liquid Chromatography with Diode Array Detection

“…The liquid desorption step is an important parameter that needs to be optimized to obtain the complete desorption of the analytes from the sorbent phase 14 and avoid the carryover effect. In this study, MeOH, ACN and acetone, along with mixtures of these solvents, were evaluated through a simplex-lattice design.…”

“…In addition, DPX-based approaches generally exhibit lower analysis cost compared with SPE. 14,15 The aim of this study was to develop a reliable, environmentally friendly and low-cost DPX-based analytical method for the multiclass determination of 12 pesticides with different physicochemical properties. The method proposed was fully optimized and validated in-house using HPLC-DAD.…”

Modern Approaches to Preparation of Body Fluids for Determination of Bioactive Compounds

“…Recently, some miniaturized concepts for chromatographic analysis involving a pipette tip and spin column were reviewed [19]. However, until now, their application to routine analysis is limited mainly due to the small number of commercially available extracting materials and the higher cost compared to traditional SPE columns [20].…”

Desenvolvimento de fases extratoras seletivas para técnicas de microextração para a determinação de fármacos e endocanabinóide em amostras de plasma por LC-MS/MS

“…Após o condicionamento, a amostra líquida (ou extrato) é aspirada para o interior da ponteira e misturada à fase extratora por meio da entrada de ar através da ponta da ponteira. A subsequente aspiração de ar resulta em uma completa mistura da amostra com a fase extratora, formando uma suspensão, e permitindo assim uma rápida e eficiente extração (BORDIN et al, 2016 A primeira descrição da DP foi feita quase dois séculos atrás, no entanto, somente no início da década de 60 que pesquisadores conseguiram identificar as primeiras alterações patológicas e bioquímicas no cérebro de indivíduos doentes, que caracterizariam essa doença.…”