Disposable superoxide dismutase biosensors based on gold covered polycaprolactone fibers for the detection of superoxide in cell culture media

Sanz, Caroline G.; Onea, Melania; Aldea, Anca; Bârsan, Mădălina M.

doi:10.1016/j.talanta.2022.123255

Cited by 11 publications

(7 citation statements)

References 44 publications

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“…7 D) [ 165 ]. Gold-sputtered PCL nanofibrous membranes exhibited higher sensitivity of hydrogen peroxide detection compared to similar Au layer-deposited Si/SiO 2 wafers due to their higher surface area [ 184 ]. Upon immobilizing of superoxide dismutase by chemically crosslinked cysteine, the PCL/Au fiber presented a sensitivity of 16.1 μA mM −1 cm −2 , a LOD of 1.9 μM detecting H 2 O 2 and recoverable determination of superoxide in cell culture media.…”

Section: Conductive Fibers In Implantable Bioelectronicsmentioning

confidence: 99%

Conductive fibers for biomedical applications

Wei

Wang

Shan

et al. 2023

Bioactive Materials

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Section: Conductive Fibers In Implantable Bioelectronicsmentioning

confidence: 99%

Conductive fibers for biomedical applications

Wei

Wang

Shan

et al. 2023

Bioactive Materials

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“…the detection of glucose, and the process could be monitored, which is the main advantage of using this biosensor. The developed biosensor (p-PDA)/CNTs/Au MEA possesses acceptable characteristics such as high detection sensitivity, exceptional reproducibility, and excellent shelf life [115]. In another study, Ag, reduced graphene oxide, and chitosan were used to fabricate a nanocomposite-based biosensor, and acetylcholinesterase enzyme was immobilized on the Ag/rGO/CS matrix.…”

Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

Kilic,

Singh,

Keles

et al. 2023

Biosensors

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Electrochemistry is a genuinely interdisciplinary science that may be used in various physical, chemical, and biological domains. Moreover, using biosensors to quantify biological or biochemical processes is critical in medical, biological, and biotechnological applications. Nowadays, there are several electrochemical biosensors for various healthcare applications, such as for the determination of glucose, lactate, catecholamines, nucleic acid, uric acid, and so on. Enzyme-based analytical techniques rely on detecting the co-substrate or, more precisely, the products of a catalyzed reaction. The glucose oxidase enzyme is generally used in enzyme-based biosensors to measure glucose in tears, blood, etc. Moreover, among all nanomaterials, carbon-based nanomaterials have generally been utilized thanks to the unique properties of carbon. The sensitivity can be up to pM levels using enzyme-based nanobiosensor, and these sensors are very selective, as all enzymes are specific for their substrates. Furthermore, enzyme-based biosensors frequently have fast reaction times, allowing for real-time monitoring and analyses. These biosensors, however, have several drawbacks. Changes in temperature, pH, and other environmental factors can influence the stability and activity of the enzymes, affecting the reliability and repeatability of the readings. Additionally, the cost of the enzymes and their immobilization onto appropriate transducer surfaces might be prohibitively expensive, impeding the large-scale commercialization and widespread use of biosensors. This review discusses the design, detection, and immobilization techniques for enzyme-based electrochemical nanobiosensors, and recent applications in enzyme-based electrochemical studies are evaluated and tabulated.

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“…Natural enzymes, such as horseradish peroxidase (HRP), alkaline phosphatase (ALP), acetylcholinesterase (AChE), glucose oxidase (GOx), superoxide dismutase (SOD), and laccase have also been used for signal amplification. Wang et al prepared a mesoporous core–shell palladium@platinum (Pd@Pt) nanozyme and Pd@Pt-HRP complex (HRP coupled with Pd@Pt nanozymes) as signal amplifiers in a Staphylococcus aureus colorimetric immunoassay .…”

Section: Signal Amplification Strategymentioning

confidence: 99%

Recent Advances in Design and Application of Nanomaterials-Based Colorimetric Biosensors for Agri-food Safety Analysis

Zhou,

Liu,

et al. 2023

ACS Omega

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A colorimetric sensor detects an analyte by utilizing the optical properties of the sensor unit, such as absorption or reflection, to generate a structural color that serves as the output signal to detect an analyte. Detecting the refractive index of an analyte by recording the color change of the sensor structure on its surface has several advantages, including simple operation, low cost, suitability for onsite analysis, and real-time detection. Colorimetric sensors have drawn much attention owing to their rapidity, simplicity, high sensitivity and selectivity. This Review discusses the use of colorimetric sensors in the food industry, including their applications for detecting food contaminants. The Review also provides insight into the scope of future research in this area.

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Disposable superoxide dismutase biosensors based on gold covered polycaprolactone fibers for the detection of superoxide in cell culture media

Cited by 11 publications

References 44 publications

Conductive fibers for biomedical applications

Conductive fibers for biomedical applications

Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

Recent Advances in Design and Application of Nanomaterials-Based Colorimetric Biosensors for Agri-food Safety Analysis

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