Dissection of insular cortex layer 5 reveals two sublayers with opposing modulatory roles in appetitive drinking behavior

Takemoto, Makoto; Kato, Shigeki; Kobayashi, Kazuto; Song, Wen Jie

doi:10.1016/j.isci.2023.106985

Cited by 3 publications

(1 citation statement)

References 75 publications

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“…Similarly, within the insula, the localization of neurons in different layers can result in opposing effects on behaviors like appetitive drinking, depending on their projection targets. For example, layer 5 neurons in the insula can mediate both promotion and inhibition of drinking behavior, with layer 5a and 5b neurons projecting to different downstream targets with distinct functional outcomes [79]. These results may imply that specific pathways or downstream areas within the insula may play a more crucial role in regulating behaviors, suggesting that the distinct tickle-response clusters may have distinct projection targets.…”

Section: Discussionmentioning

confidence: 99%

Rat Anterior Insula Symmetrically Represents Tickling-Induced Playful Emotions

Dagher,

Ishiyama

2024

Preprint

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Social play, an integral aspect of animal behavior, is inherently associated with positive emotions, yet the neuronal underpinnings of these playful states remain inadequately explored. We examined the anterior insula's involvement in processing tickle-induced playful emotions in rats. Our findings revealed diverse patterns of insular activity during tickling, with 20% of the recorded units displaying strong activation, and another 20% exhibiting inhibition. These units responded similarly to other playful contexts, such as gentle touch and hand chase, but not to neutral locomotion. Tickle-activated units demonstrated a positive correlation of firing rates with appetitive vocalization rates, whereas tickle-inhibited units showed a negative correlation. Distinct spike waveforms were associated with the tickle response patterns, suggesting potential cell-type dependencies. However, pharmacological manipulation of the global anterior insula did not yield observable effects on play behavior in rats. Anterograde tracing revealed extensive insular projections to areas including the amygdala and nucleus accumbens. Taken together, our findings suggest that the anterior insula symmetrically represents tickle-induced playful emotional states.

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Section: Discussionmentioning

confidence: 99%

Rat Anterior Insula Symmetrically Represents Tickling-Induced Playful Emotions

Dagher,

Ishiyama

2024

Preprint

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Cannabinoids regulate an insula circuit controlling water intake

Zhao,

Covelo,

Couderc

et al. 2024

Current Biology

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The parasubthalamic nucleus refeeding ensemble delays feeding initiation and hastens water drinking

Dunning,

Lopez,

Krull

et al. 2024

Mol Psychiatry

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The parasubthalamic nucleus (PSTN) is activated by refeeding after food deprivation and several PSTN subpopulations have been shown to suppress feeding. However, no study to date directly addressed the role of PSTN neurons activated upon food access in the control of ensuing food consumption. Here we identify consumption latency as a sensitive behavioral indicator of PSTN activity, and show that, in hungry mice, the ensemble of refeeding-activated PSTN neurons drastically increases the latency to initiate refeeding with both familiar and a novel, familiar food, but does not control the amount of food consumed. In thirsty mice, this ensemble also delays sucrose consumption but accelerates water consumption, possibly reflecting anticipatory prandial thirst, with again no influence on the amount of fluid consumed. We next sought to identify which subpopulations of PSTN neurons might be driving these latency effects, using cell-type and pathway-specific chemogenetic manipulations. Our results suggest a prominent role of PSTN Tac1 neurons projecting to the central amygdala in the hindrance of feeding initiation. While PSTN Crh neurons also delay the latency of hungry mice to ingest familiar foods, they surprisingly promote the consumption of novel, palatable substances. Furthermore, PSTN Crh neurons projecting to the bed nucleus of the stria terminalis accelerate rehydration in thirsty mice. Our results demonstrate the key role of endogenous PSTN activity in the control of feeding and drinking initiation and delineate specific circuits mediating these effects, which may have relevance for eating disorders.

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Dissection of insular cortex layer 5 reveals two sublayers with opposing modulatory roles in appetitive drinking behavior

Cited by 3 publications

References 75 publications

Rat Anterior Insula Symmetrically Represents Tickling-Induced Playful Emotions

Rat Anterior Insula Symmetrically Represents Tickling-Induced Playful Emotions

Cannabinoids regulate an insula circuit controlling water intake

The parasubthalamic nucleus refeeding ensemble delays feeding initiation and hastens water drinking

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