Dissection of the interaction between human holo-transferrin and ciprofloxacin in the presence of silver nanoparticles: spectroscopic approaches

Koohzad, Fatemeh; Beigoli, Sima; Jahanshahtalab, Mahtab; Kamshad, Maryam; Darban, Reza Assaran; Chamani, Jamshidkhan

doi:10.1515/biolog-2017-0066

Cited by 22 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This value is 100-fold larger than the maximum scatter collision quenching constant for quenchers (2.0 × 10 10 M –1 s –1 ) . Therefore, the k q values showed that the quenching mechanism for Hb in the presence of SiO 2 NPs must be due to the complex formation between the proteins and SiO 2 NPs, as reported in static quenching …”

Section: Resultsmentioning

confidence: 75%

See 1 more Smart Citation

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Sabziparvar¹,

Saeedi²,

Nouri³

et al. 2018

J. Phys. Chem. B

View full text Add to dashboard Cite

Nanoparticles (NPs) have received great attention in biological and medical applications because of their unique features. However, their induced adverse effects on the biological system are not well-explored. Herein, the interaction of silicon dioxide nanoparticles (SiO NPs) with human hemoglobin (Hb) and lymphocyte cell line was evaluated under physiological conditions by multispectroscopic [intrinsic and synchronous fluorescence spectroscopy and circular dichrosim (CD)], molecular docking, and cellular [3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) and acridine orange/ethidium bromide (AO/EB) staining] methods. Transmission electron microscopy and dynamic light scattering revealed the nanosized and spherical shaped SiO particle. The fluorescence and lifetime decay results indicated that SiO NPs quenched the intrinsic intensity of Hb through a static quenching mechanism. The binding affinity of SiO NPs toward Hb was directly correlated with temperature. The sign of thermodynamic parameters demonstrated that hydrophobic forces played a pivotal role in the interaction of SiO NPs with Hb. The results of synchronous fluorescence experiments displayed that Tyr residues are moved to a more hydrophilic microenvironment. Molecular docking studies exhibited that SiO and Si NPs were bound to Hb primarily by hydrophobic residues. The findings from CD data verified no alteration in the secondary structure of Hb upon binding to SiO NPs. The human lymphocyte cell line was treated with SiO NPs at varying concentrations and time intervals and the cytotoxicity assays by MTT and AO/EB staining showed that cell viability was reduced by the SiO NP-induced apoptosis mechanism in a dose and time-dependent manner. Therefore, it may be suggested that comprehensive details regarding the interaction of NPs and biological systems such as cells and proteins can provide useful information in the development of NP-based systems.

show abstract

Section: Resultsmentioning

confidence: 75%

“…30 Therefore, the k q values showed that the quenching mechanism for Hb in the presence of SiO 2 NPs must be due to the complex formation between the proteins and SiO 2 NPs, as reported in static quenching. 31 3.4. Binding Parameters.…”

Section: The Journal Of Physical Chemistry Bmentioning

confidence: 99%

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Sabziparvar¹,

Saeedi²,

Nouri³

et al. 2018

J. Phys. Chem. B

View full text Add to dashboard Cite

show abstract

“…Previous studies have investigated ligand binding to serum albumin and have reported it on the order of 10 3 –10 5 M –1 . For the NOS–HSA system, the binding order was also found in a similar range, i.e., 1.77 × 10 4 M –1 , at 298 K. The higher magnitude of the K b value also suggested that, at in vivo conditions, NOS can bind effectively with HSA at the same temperature condition. − In addition, from Table , it can be seen that the K b also reduces with increasing temperature due to the instability of the NOS–HSA complex at higher temperatures …”

Section: Resultsmentioning

confidence: 78%

In Vitro Cytotoxicity and Interaction of Noscapine with Human Serum Albumin: Effect on Structure and Esterase Activity of HSA

et al. 2019

View full text Add to dashboard Cite

Noscapine is effective to inhibit cellular proliferation and induced apoptosis in nonsmall cell, lung, breast, lymphoma, and prostate cancer. It also shows good efficiency to skin cancer cells. In the current work, we studied the mechanism of interaction between the anticancer drug noscapine (NOS) and carrier protein human serum albumin (HSA) by using a variety of spectroscopic techniques (fluorescence spectroscopy, time-resolved fluorescence, UV−visible, fluorescence resonance energy transfer (FRET), Fourier transform infrared (FTIR), and circular dichroism (CD) spectroscopy), electrochemistry (cyclic voltammetry), and computational methods (molecular docking and molecular dynamic simulation). The steady-state fluorescence results showed that fluorescence intensity of HSA decreased in the presence of NOS via a static quenching mechanism, which involves ground state complex formation between NOS and HSA. UV−visible and FRET results also supported the fluorescence result. The corresponding thermodynamic result shows that binding of NOS with HSA is exothermic in nature, involving electrostatic interactions as major binding forces. The binding results were further confirmed through a cyclic voltammetry approach. The FRET result signifies the energy transfer from Trp214 of HSA to the NOS. Molecular site marker, molecular docking, and MD simulation results indicated that the principal binding site of HSA for NOS is site I. Synchronous fluorescence spectra, FTIR, 3D fluorescence, CD spectra, and MD simulation results reveal that NOS induced the structural change in HSA. In addition, the MTT assay study on a human skin cancer cell line (A-431) was also performed for NOS, which shows that NOS induced 80% cell death of the population at a 320 μM concentration. Moreover, the esterase-like activity of HSA with NOS was also done to determine the variation in protein functionality after binding with NOS.

show abstract

“…8 They suggested that chest pain after sustained strain may be indicative of esophageal perforation. 9,10 Although barium esophagography has up to 10% false-negative results, it is the standard method for diagnosis of esophageal perforation. If oral contrast leaks into the mediastinum, it shows esophageal perforation; otherwise, CT may be better for diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“…8 They suggested that chest pain after sustained strain may be indicative of esophageal perforation. 9,10…”

Section: Discussionmentioning

confidence: 99%

Surgical management of early and late esophageal perforation

Masoom

Dalouee

Fattahi

et al. 2018

Asian Cardiovasc Thorac Ann

View full text Add to dashboard Cite

Introduction Esophageal perforation is a rare and life-threating problem with a 10%–40% mortality rate. Early diagnosis and treatment are important for prevention of complications. Strategies for treatment of esophageal perforation have been controversial for many years, especially in cases of late presentation. Methods We prospectively studied 27 patients (12 male, 17 female, mean age 42.7 ± 17.8 years) who presented with esophageal perforation from 1996 to 2015, and evaluated the results of surgical treatment. The patients were divided into 3 groups according to time of presentation: early (<24 h), intermediate (24–72 h), and late (>72 h). We also considered the etiology and site of esophageal perforation, complications, and mortality. Results Surgery was performed in 5 patients in the early group, 7 in the moderate group, and 15 in the late group. Primary repair was carried out in 5 cases, primary repair and reinforcement with a flap in 10, esophageal resection and reconstruction in 8, and a T-tube stent was used in 4. Four patients developed a fistula postoperatively, and there was one death due to respiratory failure. No relationship was found between complications and the cause of perforation, time of presentation, or type of treatment. Conclusion In patients without sepsis, primary repair can be an option even in those presenting late after esophageal perforation, with an acceptable result.

show abstract

Dissection of the interaction between human holo-transferrin and ciprofloxacin in the presence of silver nanoparticles: spectroscopic approaches

Cited by 22 publications

References 44 publications

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

In Vitro Cytotoxicity and Interaction of Noscapine with Human Serum Albumin: Effect on Structure and Esterase Activity of HSA

Surgical management of early and late esophageal perforation

Contact Info

Product

Resources

About