Disseminated Cryptococcosis Presenting as Cellulitis Diagnosed by Laser Capture Microdissection: A Case Report and Literature Review

Song, Yinggai; Liu, Xiao; Hoog, G.S. de; Li, Ruoyu

doi:10.1007/s11046-021-00543-3

Cited by 5 publications

(5 citation statements)

References 55 publications

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“…LCM is a novel and powerful technique to select individual cells or groups of cells from complex heterozygous biological samples, especially in clinical specimens with lower fungi burden 15,30,31 . The use of LCM to specifically capture fungal hyphae may result in increasing PCR sensitivity and exclude the influence of human DNA in the diagnosis in case of low fungal abundance samples, thus improving diagnostic efficiency 16,23,32 . However, the limitation of LCM is time consuming.…”

Section: Discussionmentioning

confidence: 99%

“…Laser microdissection were performed to capture PAS‐positive fungal components such as hyphae and spores in the tissue sections under light microscopy using a Lecia LMD6000 system (Leica, Wetzlar, Germany). All the pure PAS‐positive fungal components on the slides were dissected with a 337 nm pulsed ultraviolet laser as much as possible and collected in a sample tube 23 . In addition, 4 µm thick clinical FFPE tissue sections were mounted on slides for fluorescence in situ hybridisation and immunohistochemical staining.…”

Section: Methodsmentioning

confidence: 99%

“…All the pure PAS-positive fungal components on the slides were dissected with a 337 nm pulsed ultraviolet laser as much as possible and collected in a sample tube. 23 In addition, 4 µm thick clinical FFPE tissue sections were mounted on slides for fluorescence in situ hybridisation and immunohistochemical staining.…”

Section: Dna Extractionmentioning

confidence: 99%

“…Although PCR-sequencing has been adopted as one of the di- 15,30,31 The use of LCM to specifically capture fungal hyphae may result in increasing PCR sensitivity and exclude the influence of human DNA in the diagnosis in case of low fungal abundance samples, thus improving diagnostic efficiency. 16,23,32 However, the limitation of LCM is time consuming. 24,33 However, this may also lead to a decrease of specificity in pan-fungal PCR-sequencing, especially as FFPE tissues may be contaminated by environmental spores.…”

Section: Realmentioning

confidence: 99%

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The use of combined PCR, fluorescence in situ hybridisation and immunohistochemical staining to diagnose mucormycosis from formalin‐fixed paraffin‐embedded tissues

Xiao

Song

2021

Mycoses

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Objective: To develop a comprehensive diagnostic system for mucormycosis from formalin-fixed paraffin-embedded tissues, consisting of own-designed real-time polymerase chain reaction (PCR) assays, fluorescence in situ hybridisation, and immunohistochemical staining. Methods: We designed 11 primers and probes for specific real-time PCR assays based on genome sequences, and validated the specificity by Aspergillus, Fusarium, Scedosporium, Lomentospora, Cryptococcus and Candida species. Formalin-fixed paraffin-embedded (FFPE) tissues from forty-four mouse model infected by above fungi were collected and extracted DNA by laser capture microdissection (LCM) and direct extraction methods for real-time PCR assays. In addition, seventeen clinical specimens histopathologically proven for mucormycosis were included for specific detection with the new diagnostic system. Results: The real-time PCR assays allowed detection of a minimum of 10 CFU/ml equivalent gDNA of each species. No cross-reaction with gDNA among species was noted. From mouse model specimens, the sensitivity of real-time PCR in samples extracted with LCM versus direct extraction method was 100% versus 91.43% at Mucorales level and 80% versus 45.71% at species level, respectively. The specificity was 100%. From clinical samples, LCM combined with real-time PCR can test 88.24% (15/17) of Mucorales. Sensitivities of fluorescence in situ hybridisation (FISH) and immunohistochemical staining (IHC) were 70.59% and 41.18%, respectively. Combined LCM-RT-PCR, FISH and IHC yielded positive results in all samples.Conclusions: The combination diagnostic system we developed is a cultureindependent and robust method which enables rapid species identification from FFPE tissues for timely diagnosis of mucormycosis.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Dna Extractionmentioning

confidence: 99%

Section: Realmentioning

confidence: 99%

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The use of combined PCR, fluorescence in situ hybridisation and immunohistochemical staining to diagnose mucormycosis from formalin‐fixed paraffin‐embedded tissues

Xiao

Song

2021

Mycoses

Self Cite

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“…The lack of early and rapid diagnosis is an important reason for the high disease burden of both fungal diseases. At present, the routine diagnostic methods of cryptococcosis and talaromycosis include direct microscopic examination, culture, serological examination, histopathology and molecular identification, 15,16 which have problems such as time-consuming, cumbersome and costly operation and are not suitable for promotion to low-income countries or the grassroots level. Moreover, skin lesions are common clinical symptoms and important and intuitive diagnostic indexes of talaromycosis and cryptococcosis.…”

mentioning

confidence: 99%

Application of deep learning algorithm in the recognition of cryptococcosis and talaromycosis skin lesions

Wei

Bao

et al. 2023

Mycoses

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Fungal pathogens and infections are a growing global public health concern, with those most at risk being those with underlying health conditions or weakened immune systems. In recent years, the incidence and mortality of invasive fungal infections such as cryptococcus and talaromycosis have increased with the increase in HIV infection, the use of malignant tumours, organ transplantation and immunosuppressive age. 1,2 Cryptococcosis, an invasive fungus caused by Cryptococcus, is the third most common invasive fungal infection. 3,4 Approximately one million people are infected with Cryptococcus, and 630,000 people die each year worldwide. [5][6][7][8] Talaromycosis, also with a high case fatality rate, is an invasive fungal infection caused by Talaromyces marneffei infection, which causes

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Evaluating Terrestrol A as an Inhibitor Against SARS‐CoV‐2and Invasive Fungal Pathogens: A Comprehensive Computational Analysis

Singha,

Arora,

Karmaker

et al. 2024

ChemistrySelect

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The highly contagious SARS‐CoV‐2 virus, which initiated the COVID‐19 pandemic, has resulted in a significant loss of life worldwide. While vaccines and therapeutics have helped mitigate the impact of SARS‐CoV‐2, worries still persist regarding the emergence of Variants of Concern (VOCs). Additionally, the immunosuppressive effects of SARS‐CoV‐2 and steroid treatments increase susceptibility to complex fungal co–infections (e. g., aspergillosis and candidiasis), complicating treatment and escalating mortality rates. These circumstances highlight the urgent need for novel therapeutics. Thus, this study explores gentisyl alcohol and its derivatives as inhibitors of SARS‐CoV‐2 and fungal pathogens (Aspergillus fumigatus and Candida auris) via in‐silico methodologies. Initial multi–targeted docking simulations revealed that Terrestrol A(10) exhibited highly promising results, displaying the most favourable MolDock‐scores against all targets of interest, particularly against PDB ID 4K90 and 7CR5. The subsequent assessment of Terrestrol A(10) for their druglikeness, ADME, toxicity profiling, bioactivity, and PASS prediction also yielded highly favourable results. Structural dynamics analysis revealed stable yet slightly unfolding complexes, affirming their integrity. DFT studies also demonstrated favourable reactivity of Terrestrol A(10). This investigation identifies Terrestrol A(10) as a highly promising inhibitor against SARS‐CoV‐2 and fungal pathogens, laying the groundwork for in vitro and in vivo validation of its efficacy and clinical utility.

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Disseminated Cryptococcosis Presenting as Cellulitis Diagnosed by Laser Capture Microdissection: A Case Report and Literature Review

Cited by 5 publications

References 55 publications

The use of combined PCR, fluorescence in situ hybridisation and immunohistochemical staining to diagnose mucormycosis from formalin‐fixed paraffin‐embedded tissues

The use of combined PCR, fluorescence in situ hybridisation and immunohistochemical staining to diagnose mucormycosis from formalin‐fixed paraffin‐embedded tissues

Application of deep learning algorithm in the recognition of cryptococcosis and talaromycosis skin lesions

Evaluating Terrestrol A as an Inhibitor Against SARS‐CoV‐2and Invasive Fungal Pathogens: A Comprehensive Computational Analysis

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