Dissolving microneedles for long-term storage and transdermal delivery of extracellular vesicles

Bui, Van; Son, Sangyoung; Xavier, Wilbin; Nguyen, Van Quy; Jung, Jae Min; Lee, Jungmi; Shin, Sol; Um, Wooram; An, Jae Yoon; Kim, Chan Ho; Song, Yeari; Li, Yuce; Park, Jae Hyung

doi:10.1016/j.biomaterials.2022.121644

Cited by 33 publications

(25 citation statements)

References 48 publications

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“…The dissolvable material HA was selected as matrices for MN because it is a skin intrinsic composition with high security. 37,38 Various molecular weights and solubility of HA were tried, as shown from the data in Table 3. Depending on whether the MN matrices can be formed, 1 × 10 5 molecular weight HA with 5% solubility, 1 × 10 4 molecular weight HA with 20% solubility, 1 × 10 4 molecular weight HA with 15% solubility, and 1 × 10 4 molecular weight HA with 10% solubility were chosen for further fabrication.…”

Section: Resultsmentioning

confidence: 99%

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A lipid–polymer hybrid nanoparticle (LPN)-loaded dissolving microneedle patch for promoting hair regrowth by transdermal miR-218 delivery

Zhao

Wang

et al. 2023

Biomater. Sci.

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Section: Resultsmentioning

confidence: 99%

Section: Fabrication and Characterization Of Mn/lpns/mir-218mentioning

confidence: 99%

A lipid–polymer hybrid nanoparticle (LPN)-loaded dissolving microneedle patch for promoting hair regrowth by transdermal miR-218 delivery

Zhao

Wang

et al. 2023

Biomater. Sci.

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“…Human skin is constructed with many proteins, among which Type I collagen (COL1) and elastin (ELN) that are derived from fibroblasts and constitute extracellular matrix (ECM) play a key role in providing strength and elasticity to the human skin and body ( Lee et al, 2022 ). The expressions of COL1 and ELN can well reflect the ability of fibroblast to produce ECM that is related to process of wound healing and scar formation ( Bui et al, 2022 ). qPCR and WB analysis showed that 48 h after the treatment with QCT@GNP or QCT@GNP-Ga, both the mRNA and protein levels of COL1 and ELN were significantly higher than those in the GNP group ( Figures 2E–G ).…”

Section: Resultsmentioning

confidence: 99%

Gallium-modified gelatin nanoparticles loaded with quercetin promote skin wound healing via the regulation of bacterial proliferation and macrophage polarization

Yang

Shi

Zhou

et al. 2023

Front. Bioeng. Biotechnol.

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Background: Wound healing is a complicated process involving multiple cell components and can help the re-establishment of the skin’s barrier function. Previous studies have pointed out that bacterial infection and sustained inflammatory reactions are the main causes of the delay of wound closure and scar formation during wound healing. The effect of current approaches for scar-free wound repair still faces many challenges, and alternative therapeutic methods are urgently needed to be established.Methods: The basic characteristics of the new-designed nanoparticles were clarified through the characterization of the material. The biocompatibility of the nanoparticles, as well as its effect on fibroblast function, anti-bacterial capacity, inflammation suppressive role, and the underlying mechanism were further verified by a panel of biochemical assays in vitro. Ultimately, pre-clinical rat model was employed to testify its role in wound healing and scar formation in vivo.Results: Firstly, gallium-modified gelatin nanoparticles loaded with quercetin was successfully established, displaying good biocompatibility and facilitative effect on fibroblast function. In addition, the nanoparticles showed prominent anti-bacterial and inflammation-suppressive effects. What’s more important, the nanoparticles could also induce the polarization of macrophages from M1 to M2 phenotype to exert its inflammatory inhibitory role through TGF-β/Smad signaling pathway. Ultimately, in vivo experiment showed that the nanoparticles could effectively promote wound repair and inhibit scar formation during the process of wound healing.Conclusion: Taken together, the new nanoparticles have good anti-bacterial and anti-scar formation effects and great potential in the field of skin wound repair, which provides a promising therapeutic strategy for wound treatment.

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“…[138] Commonly used cryoprotectants such as DMSO and glycerol promote EV lysis or aggregation, so Gorgens et al [139] optimized a HEPES buffer using alginate and albumin, and even after five freeze-thaw cycles at -80 °C storage conditions, the EVs and their contents remained intact. And researchers dispersed EVs in the concentrated hyaluronic acid solution and removed water by evaporation under continuous airflow to obtain solid-form EVs, called EV-microneedles, which remained stable after storing for six months, [140] making the extension of EV clinical applications like oral capsules possible.…”

Section: New Storage Of Evs and Boosting Secretionmentioning

confidence: 99%

Engineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications

Wang

et al. 2023

Advanced Science

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Extracellular vesicles (EVs) are transport vesicles secreted by living cells and released into the extracellular environment. Recent studies have shown that EVs serve as "messengers" in intercellular and inter-organismal communication, in both normal and pathological processes. EVs, as natural nanocarriers, can deliver bioactivators in therapy with their endogenous transport properties. This review article describes the engineering EVs of sources, isolation method, cargo loading, boosting approach, and adjustable targeting of EVs. Furthermore, the review summarizes the recent progress made in EV-based delivery systems applications, including cancer, cardiovascular diseases, liver, kidney, nervous system diseases, and COVID-19 and emphasizes the obstacles and challenges of EV-based therapies and possible strategies.

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Dissolving microneedles for long-term storage and transdermal delivery of extracellular vesicles

Cited by 33 publications

References 48 publications

A lipid–polymer hybrid nanoparticle (LPN)-loaded dissolving microneedle patch for promoting hair regrowth by transdermal miR-218 delivery

A lipid–polymer hybrid nanoparticle (LPN)-loaded dissolving microneedle patch for promoting hair regrowth by transdermal miR-218 delivery

Gallium-modified gelatin nanoparticles loaded with quercetin promote skin wound healing via the regulation of bacterial proliferation and macrophage polarization

Engineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications

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