Distinct and Overlapping Functions of Miscanthus sinensis MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis

Golfier, Philippe; Ermakova, Olga; Unda, Faride; Murphy, Emily K.; Xie, Jianbo; He, Feng; Zhang, Wan; Lohmann, Jan U.; Mansfield, Shawn D.; Rausch, Thomas; Wolf, Sebastián

doi:10.3390/ijms222212395

Cited by 6 publications

(1 citation statement)

References 48 publications

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“…As one of the largest transcription factor families in the plants, MYB TFs play key roles in regulating the phenylpropanoid pathway and the biosynthesis of cellulose/lignin ( Golfier et al, 2021 ; Xiao et al, 2021 ). In this study, four MYB TFs (CL.50841.1, CL.61023.5, CL.61753.1, and CL.71078.1) were preferentially expressed in the xylem.…”

Section: Discussionmentioning

confidence: 99%

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analyses Provide Comprehensive Insight Into Molecular Mechanisms of Cellulose and Lignin Biosynthesis in Cunninghamia lanceolata

Zhuang

Lin

et al. 2022

Front. Plant Sci.

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Cunninghamia lanceolata is an essential timber species that provide 20%–30% raw materials for China’s timber industry. Although a few transcriptomes have been published in C. lanceolata, full-length mRNA transcripts and regulatory mechanisms behind the cellulose and lignin biosynthesis have not been thoroughly investigated. Here, PacBio Iso-seq and RNA-seq analyses were adapted to identify the full-length and differentially expressed transcripts along a developmental gradient from apex to base of C. lanceolata shoots. A total of 48,846 high-quality full-length transcripts were obtained, of which 88.0% are completed transcriptome based on benchmarking universal single-copy orthologs (BUSCO) assessment. Along stem developmental gradient, 18,714 differentially expressed genes (DEGs) were detected. Further, 28 and 125 DEGs were identified as enzyme-coding genes of cellulose and lignin biosynthesis, respectively. Moreover, 57 transcription factors (TFs), including MYB and NAC, were identified to be involved in the regulatory network of cellulose and lignin biosynthesis through weighted gene co-expression network analysis (WGCNA). These TFs are composed of a comparable regulatory network of secondary cell wall formation in angiosperms, revealing a similar mechanism may exist in gymnosperms. Further, through qRT-PCR, we also investigated eight specific TFs involved in compression wood formation. Our findings provide a comprehensive and valuable source for molecular genetics breeding of C. lanceolata and will be beneficial for molecular-assisted selection.

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Section: Discussionmentioning

confidence: 99%

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analyses Provide Comprehensive Insight Into Molecular Mechanisms of Cellulose and Lignin Biosynthesis in Cunninghamia lanceolata

Zhuang

Lin

et al. 2022

Front. Plant Sci.

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Targeted plant improvement through genome editing: from laboratory to field

et al. 2021

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Key message This review illustrates how far we have come since the emergence of GE technologies and how they could be applied to obtain superior and sustainable crop production. Abstract The main challenges of today’s agriculture are maintaining and raising productivity, reducing its negative impact on the environment, and adapting to climate change. Efficient plant breeding can generate elite varieties that will rapidly replace obsolete ones and address ongoing challenges in an efficient and sustainable manner. Site-specific genome editing in plants is a rapidly evolving field with tangible results. The technology is equipped with a powerful toolbox of molecular scissors to cut DNA at a pre-determined site with different efficiencies for designing an approach that best suits the objectives of each plant breeding strategy. Genome editing (GE) not only revolutionizes plant biology, but provides the means to solve challenges related to plant architecture, food security, nutrient content, adaptation to the environment, resistance to diseases and production of plant-based materials. This review illustrates how far we have come since the emergence of these technologies and how these technologies could be applied to obtain superior, safe and sustainable crop production. Synergies of genome editing with other technological platforms that are gaining significance in plants lead to an exciting new, post-genomic era for plant research and production. In previous months, we have seen what global changes might arise from one new virus, reminding us of what drastic effects such events could have on food production. This demonstrates how important science, technology, and tools are to meet the current time and the future. Plant GE can make a real difference to future sustainable food production to the benefit of both mankind and our environment.

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PeMYB26, an R2R3-MYB transcription factor, positively regulates lignin deposition in Moso bamboo

Wang,

Chen,

Zhu

et al. 2024

Plant Growth Regul

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Distinct and Overlapping Functions of Miscanthus sinensis MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis

Cited by 6 publications

References 48 publications

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analyses Provide Comprehensive Insight Into Molecular Mechanisms of Cellulose and Lignin Biosynthesis in Cunninghamia lanceolata

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analyses Provide Comprehensive Insight Into Molecular Mechanisms of Cellulose and Lignin Biosynthesis in Cunninghamia lanceolata

Targeted plant improvement through genome editing: from laboratory to field

PeMYB26, an R2R3-MYB transcription factor, positively regulates lignin deposition in Moso bamboo

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