Distinct, but not completely separate spatial transport routes in the nuclear pore complex

Yang, Weidong

doi:10.4161/nucl.24874

Cited by 32 publications

(30 citation statements)

References 89 publications

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“…1 C-D). This map covers the majority of FG-domains of all native FG-Nups since this spatial distribution of the FG-domains agrees well with the full spectrum of the distribution of all FG-Nups in vivo obtained by SPEED microscopy [71][72][73][74][75] (Fig. 1).…”

Section: The Native Conformation Of the Fg-nups Barrier In The Npcssupporting

confidence: 64%

“…To distinguish these models and to provide direct evidence for the location of the transport routes for both passive diffusion of small molecules and for the facilitated diffusion of large molecules, efforts have been taken to map the passive diffusion sites and the interaction sites of FG-Nups with NTRs in real-time in vivo traffic conditions. 70,71,75 The data from our 3D mapping identified that the native FG-Nups are not evenly distributed, but instead clustered in certain spatial locations; and that the interactions of FG-Nups with NTRs as well as the distribution of small molecules along the NPC channel clearly demonstrated that the central channel axial pore is the passive diffusion route and the peripheral regions around the central channel are the sites for facilitated diffusion. These two transport routes are largely distinct, with certain overlap, dependent on the size of transiting molecules.…”

Section: Distinct Transport Routes and Proposed Transport Modelsmentioning

confidence: 99%

“…These two transport routes are largely distinct, with certain overlap, dependent on the size of transiting molecules. 70,71,75 Competitions between NTRs…”

Section: Distinct Transport Routes and Proposed Transport Modelsmentioning

confidence: 99%

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The selective permeability barrier in the nuclear pore complex

Goryaynov

Yang

2016

Nucleus

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The nuclear pore complex (NPC) mediates the shuttle transport of macromolecules between the nucleus and cytoplasm in eukaryotic cells. The permeability barrier formed by intrinsically disordered phenylalanine-glycine-rich nucleoporins (FG-Nups) in the NPC functions as the critical selective control for nucleocytoplasmic transport. Signal-independent small molecules (< 40 kDa) passively diffuse through the pore, but passage of large cargo molecules is inhibited unless they are chaperoned by nuclear transport receptors (NTRs). NTRs are capable of interacting with FG-Nups and guide the cargos to cross the barrier by facilitated diffusion. The native conformation of the FGNups permeability barrier and the competition among multiple NTRs interacting with this barrier in the native NPCs are the 2 core questions still being highly debated in the field. Recently, we applied high-speed super-resolution fluorescence microscopy to map out the natural structure of the FGNups barrier and determined the competition among multiple NTRs as they interact with the barrier in the native NPCs. In this extra-view article, we will review the current understanding in the configuration and function of FG-Nups barrier and highlight the new evidence obtained recently to answer the core questions in nucleocytoplasmic transport.

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Section: The Native Conformation Of the Fg-nups Barrier In The Npcssupporting

confidence: 64%

Section: Distinct Transport Routes and Proposed Transport Modelsmentioning

confidence: 99%

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The selective permeability barrier in the nuclear pore complex

Goryaynov

Yang

2016

Nucleus

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“…There are two routes of transport to nucleus. [48] around the central channels [49,50], by interacting with the phenylalanineglycine (F-G) nucleoporins [51]. Till now, researchers have rationally designed both soft and hard nanosystems with suitable size for the efficient entry to nucleus.…”

Section: Translocation and Triggered Drug Release Of Bio-cage@ru In Nmentioning

confidence: 99%

A multifunctional DNA origami as carrier of metal complexes to achieve enhanced tumoral delivery and nullified systemic toxicity

et al. 2016

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“…The nuclear pore complex (NPC), embedded in the nuclear envelope (NE), is a complex assembly of proteins that forms a gateway between the nucleus and the cytoplasm of eukaryotic cells. The number of NPCs in the nuclear envelope varies from hundreds (yeast) to thousands (human cells) [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. As the major exchange pathway for proteins and genetic materials, the NPC (as a whole or its various subunits) is closely involved in the regulation and control of many cellular processes [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Mapping of mRNA Export through the Nuclear Pore Complex

Schnell

Yang

2014

Genes

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The locations of transcription and translation of mRNA in eukaryotic cells are spatially separated by the nuclear envelope (NE). Plenty of nuclear pore complexes (NPCs) embedded in the NE function as the major gateway for the export of transcribed mRNAs from the nucleus to the cytoplasm. Whereas the NPC, perhaps one of the largest protein complexes, provides a relatively large channel for macromolecules to selectively pass through it in inherently three-dimensional (3D) movements, this channel is nonetheless below the diffraction limit of conventional light microscopy. A full understanding of the mRNA export mechanism urgently requires real-time mapping of the 3D dynamics of mRNA in the NPC of live cells with innovative imaging techniques breaking the diffraction limit of conventional light microscopy. Recently, super-resolution fluorescence microscopy and single-particle tracking (SPT) techniques have been applied to the study of nuclear export of mRNA in live cells. In this review, we emphasize the necessity of 3D mapping techniques in the study of mRNA export, briefly summarize the feasibility of current 3D imaging approaches, and highlight the new features of mRNA nuclear export elucidated with a newly developed 3D imaging approach combining SPT-based super-resolution imaging and 2D-to-3D deconvolution algorithms.

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Distinct, but not completely separate spatial transport routes in the nuclear pore complex

Cited by 32 publications

References 89 publications

The selective permeability barrier in the nuclear pore complex

The selective permeability barrier in the nuclear pore complex

A multifunctional DNA origami as carrier of metal complexes to achieve enhanced tumoral delivery and nullified systemic toxicity

Three-Dimensional Mapping of mRNA Export through the Nuclear Pore Complex

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