Distinct Dynamics of Stem and Progenitor Cells in Blood of Polytraumatized Patients

Vogel, Mona; Christow, Hannes; Manz, Isabel; Denkinger, Michael; Amoah, Amanda; Schütz, Desirée; Brown, Andreas; Möhrle, Bettina; Schaffer, Annika; Kalbitz, Miriam; Gebhard, Florian; Mayer, Benjamin; Huber‐Lang, Markus; Geiger, Hartmut

doi:10.1097/shk.0000000000001198

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…A nadir of HSPCs in PB was also observed in polytrauma (PT) patients, although at 24 h post-PT. The overall dynamics of the frequency of SPCs in mice upon TXT is therefore somewhat similar to the inverse bell-shaped pattern of the frequency of HSCPs in PB of polytrauma patients that was recently described by us (23), but with an accelerated change in mice.…”

Section: Resultssupporting

confidence: 84%

A Limited Role for AMD3100 Induced Stem Cell Mobilization for Modulation of Thoracic Trauma Outcome

Vogel

Möhrle

Sakk

et al. 2022

Shock

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Thoracic trauma is a major cause of mortality due to the associated inflammatory acute respiratory distress syndrome and morbidity due to impaired tissue regeneration. Trauma-induced lung inflammation is characterized by the early recruitment of cells with pro-or anti-inflammatory activity to the lung. Therapeutic interventions reducing the level of tissue inflammation may result in decreased tissue damage and improved healing and recovery. Stem cells might be able to improve trauma outcome via immunomodulation or by enhancing tissue regeneration. Here, we describe the migratory dynamics of murine mesenchymal, hematopoietic and endothelial stem and progenitor cells (SPCs) as well as mature inflammatory cells (monocytes, neutrophils, lymphocytes) to peripheral blood (PB) and lung tissue between 0.2 and 48 h post-blunt chest trauma (TXT). We demonstrate that the kinetics of immune cell and SPC distribution upon trauma are both cell-type and tissue-dependent. We identified a transient, early increase in the number of inflammatory cells in PB and lung at 2 h post-TXT and a second wave of infiltrating SPCs in lungs by 48 h after TXT induction, suggesting a role for SPCs in tissue remodeling after the initial inflammatory phase. Cxcl12/Cxcr4 blockade by AMD3100 within the first 6 h after TXT, while inducing a strong and coordinated mobilization of SPCs and leukocytes to PB and lung tissue, did not significantly affect TXT associated inflammation or tissue damage as determined by inflammatory cytokine levels, plasma markers for organ function, lung cell proliferation and survival, and myofibroblast/fibroblast ratio in the lung. Further understanding the dynamics of the distribution of endogenous SPCs and inflammatory cells will therefore be indispensable for stem cell-based or immunomodulation therapies in trauma.

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Section: Resultssupporting

confidence: 84%

A Limited Role for AMD3100 Induced Stem Cell Mobilization for Modulation of Thoracic Trauma Outcome

Vogel

Möhrle

Sakk

et al. 2022

Shock

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“…AMPC were isolated and cultured from human PB, which is a heterogeneous pool of cells with several cell types that show some stem markers of HSPCs/MNCs, MSCs, EPCs, and VSELs. We suggest 2 major reasons for the difference in expression between AMPC and other HSPCs/MNCs, MSCs, EPCs, HSCs, [ 24 , 25 ] and VSELs [ 5 , 10 , 26 , 27 ] stem cells, including the addition of growth factors for culture and granulocyte colony-stimulating factor treatment for enriched stem cell collection for other stem cells, [ 5 , 28 ] but not for AMPC. Our results indicated that CD29 and CD44 achieved 90% to 100% expression ratios in AMPC (Table 3 ).…”

Section: Discussionmentioning

confidence: 99%

Culture of leukocyte-derived cells from human peripheral blood: Increased expression of pluripotent genes OCT4, NANOG, SOX2, self-renewal gene TERT and plasticity

et al. 2023

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There are few stem cells in human peripheral blood (PB). Increasing the population and plasticity of stem cells in PB and applying it to regenerative medicine require suitable culture methods. In this study, leukocyte populations 250 mL of PB were collected using a blood separator before that were cultured in optimal cell culture medium for 4 to 7 days. After culturing, stemness characteristics were analyzed, and red blood cells were removed from the cultured cells. In our results, stemness markers of the leukocyte populations Sca-1 + CD45 + , CD117 + CD45 + , and very small embryonic-like stem cells CD34 + Lin − CD45 − and CXCR4 + Lin − CD45 − were significantly increased. Furthermore, the expression of stem cell genes OCT4 (POU5F1), NANOG, SOX2, and the self-renewal gene TERT was analyzed by quantitative real-time polymerase chain reaction in these cells, and it showed a significant increase. These cells could be candidates for multi-potential cells and were further induced using transdifferentiation culture methods. These cells showed multiple differentiation potentials for osteocytes, nerve cells, cardiomyocytes, and hepatocytes. These results indicate that appropriate culture methods can be applied to increase expression of pluripotent genes and plasticity. Leukocytes of human PB can be induced to trans-differentiate into pluripotent potential cells, which will be an important breakthrough in regenerative medicine.

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“…Essa interação é bidirecional, de modo que a inflamação não apenas leva à ativação da coagulação, como também ativa as proteases de coagulação, que regulam de forma direta e indireta a inflamação. Em alguns casos, como na infecção grave ou sepse, a ativação sistêmica da inflamação e coagulação podem se manifestar de forma órgão-específica, o que pode ser relevante para a disfunção orgânica subsequente (VOLPIN et al, 2014;GUISASOLA et al, 2018;VOGEL et al, 2019).…”

Section: Revisãounclassified

“…Se não for adequadamente neutralizada pela fibrinólise, a deposição de fibrina pode causar obstrução difusa da microvasculatura. Além disso, a expressão de VOGEL et al, 2019). Existe a correlação da resposta de IL-6 e IL-10 no trauma com gravidade e mortalidade da lesão, nas primeiras 24 horas além disso outros estudos também mostraram o papel da IL-6 no diagnóstico precoce de complicações pós-trauma (OSUKA et al, 2014;DEKKER;KRIJNEN;SCHIPPER, 2016;NAMAS et al, 2015;KHURANA et al, 2019).…”

Section: Revisãounclassified

Análise Da Correlação De Citocinas Inflamatórias E Civd Em Pacientes Politraumatizados

BARBOSA¹,

Nogueira²,

Souza³

2022

RECISATEC

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A coagulação intravascular disseminada (CIVD) é caracterizada pela ativação in vivo do sistema de coagulação, o que resulta na deposição intravascular de fibrina e sangramento excessivo. O paciente politraumatizado sofre de um quadro de inflamação sistêmica mediada por citocinas inflamatórias. Nesse contexto, o objetivo do presente trabalho é relacionar as citocinas inflamatórias com o quadro de coagulação intravascular disseminada em pacientes politraumatizados. A revisão de literatura foi realizada a partir do levantamento de artigos científicos encontrados nas principais plataformas de busca de artigos científicos: ScienceDirect, PubMed, SciELO e BIREME, no período de março de 2021 a março de 2022, usando os descritores: traumatismo múltiplo; Citocinas; Coagulação Intravascular Disseminada. A resposta inflamatória sistêmica por estar associada à CIVD onde as citocinas e quimiocinas atuam como mediadores-chave. Há ampla evidência de que há considerável cross-talk entre ativação inflamatória e atividade hemostática. Essa interação é bidirecional, de modo que a inflamação não apenas leva à ativação da coagulação, mas ativa as proteases de coagulação também regulam de forma importante a inflamação. Mudanças nas concentrações das citocinas G-SCF, Gro-α, IL-6, IL-8, IL-10 e MCP-1 correlacionaram-se com gravidade do quadro de pacientes politraumatizados sugerindo que essas citocinas podem estar desempenhando papéis protetores na imunopatogênese de pacientes politraumatizados, sendo assim, todas essas citocinas podem ser potenciais biomarcadores confiáveis para predizendo o desenvolvimento da síndrome de CVID em pacientes com politraumatismo.

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Distinct Dynamics of Stem and Progenitor Cells in Blood of Polytraumatized Patients

Abstract: Supplemental Digital Content is available in the text

Cited by 6 publications

References 36 publications

A Limited Role for AMD3100 Induced Stem Cell Mobilization for Modulation of Thoracic Trauma Outcome

A Limited Role for AMD3100 Induced Stem Cell Mobilization for Modulation of Thoracic Trauma Outcome

Culture of leukocyte-derived cells from human peripheral blood: Increased expression of pluripotent genes OCT4, NANOG, SOX2, self-renewal gene TERT and plasticity

Análise Da Correlação De Citocinas Inflamatórias E Civd Em Pacientes Politraumatizados

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