Distinct Effects of Imperatorin on Allergic Rhinitis: Imperatorin Inhibits Caspase-1 Activity In Vivo and In Vitro

Abstract

Because imperatorin (IPT), the furanocoumarins exhibits antiinflammatory activity, we reasoned that IPT might modulate the allergic rhinitis (AR). The aim of this study was to analyze the regulation of AR by IPT. Here, we show the effect and mechanism of IPT in an ovalbumin (OVA)-induced AR model. The number of rubs after the OVA challenge in the OVA-sensitized mice was significantly higher than that in the OVA-unsensitized mice. The increased number of rubs was inhibited by the oral administration of IPT. The increased levels of IgE and histamine in the OVA-sensitized mice were reduced by IPT administration. The levels of interferon-␥ were enhanced, whereas the levels of interleukin (IL)-4 were reduced on the spleen tissue of the IPT-administered AR mice. Protein levels of IL-1␤, macrophage inflammatory protein-2, intercellular adhesion molecule-1, and cyclooxygenase-2 were reduced by IPT administration in the nasal mucosa of the OVA-sensitized mice. In the IPT-administered mice, the number of eosinophils and mast cells infiltration increased by OVA-sensitization were also decreased. In addition, IPT inhibited caspase-1 activity in the same nasal mucosa tissue. In activated human mast cells, the receptor-interacting protein 2 (RIP2), IB kinase (IKK)-␤, nuclear factor-B (NF-B)/ RelA, and caspase-1 activation were increased, but increased RIP2, IKK-␤, NF-B/RelA, and caspase-1 activation were inhibited by the treatment of IPT. In addition, IPT inhibited caspase-1 activity and IL-1␤ production in IgE-stimulated bone marrowderived mast cells. We can conclude that IPT exerts significant effects by regulating of caspase-1 activation in AR animal and in vitro models.

Anti-allergic Effect of Intranasal Administration of Type-A Procyanidin Polyphenols Based Standardized Extract of Cinnamon Bark in Ovalbumin Sensitized BALB/c Mice

“…In the present study, we observed large number of hemosiderin leyden macrophages as well as thick fibrous capsule in the section of spleen and corresponding enlargement of spleen in AR control mice. Our observations are in line with previous reports of splenic enlargement and cellular infiltration (neutrophils, eosinophils and mast cells) caused by OVA sensitization in rats (Oh et al , ). TAPP‐CZ treatment was found to significantly prevent OVA‐induced splenic enlargement and cellular infiltration in present study indicating beneficial effects against immune inflammation such as AR.…”

NLRP3 inflammasome: A likely target for the treatment of allergic diseases

“…Moreover, Gami‐hyunggyeyeongyotang, which is a polyherbal medicine derived from an oriental prescription traditionally used for the treatment of allergic diseases, can attenuate allergic inflammation in AR by suppressing IL‐1β and caspase‐1 expression via the inflammatory pathway in human mast cells . Several other research studies have revealed that the levels of IL‐1β and caspase‐1 in the serum or nasal mucosa tissue were increased in the OVA‐induced AR mice compared with those in the control mice and that the administration of anti‐allergic drugs inhibited IL‐1β and caspase‐1 expression and attenuated the allergic symptoms …”

Biyuanling suppresses the toluene-2, 4-diisocyanate induced allergic rhinitis in guinea pigs

“…The development of AR is a rather complicated process involving sensitization by allergens, expression of antigen specific IgE, degranulation of mast cells and release of inflammatory mediators, such as histamine, tryptase, prostaglandins and leukotrienes, and intramucosal inflammatory cell infiltrations. TDI is a highly active chemical agent that can induce the activation of the mast cells, leading to a hypersensitive reaction in the nasal cavity of guinea pigs [ 24 ]. When treated with BLG at different doses, AR symptoms of the experimental animals were obviously alleviated [ 25 ].…”