Distinct expression pattern of miRNAs in Marek's disease virus infected-chicken splenic tumors and non-tumorous spleen tissues

Li, Zhijie; Zhang, Yanping; Yang, Li; Zheng, Huiwen; Zheng, Yongsheng; Liu, Changjun

doi:10.1016/j.rvsc.2014.04.003

Cited by 21 publications

(16 citation statements)

References 28 publications

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“…Li et al . (2014) showed significant differential expression of 79 miRNAs that were regarded as sensitive to infection by MDV and found two clades of chicken miRNAs that were considered to be signatures for MDV infection and tumorigenesis 10 . Gga-miR-26a was downregulated in MDV-induced tumors 11 and mediated IL-2 expression in transformed avian lymphocyte lines 5 .…”

Section: Introductionmentioning

confidence: 99%

Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1

Zhao

Han

et al. 2017

Sci Rep

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Marek’s disease (MD), caused by Marek’s disease virus (MDV), is a lymphotropic neoplastic disease. Previous miRNAome analysis showed gga-miR-219b was significantly downregulated in MDV-induced lymphoma, and one of its potential target genes, B-cell chronic lymphocytic /lymphoma 11B (BCL11B) was predicted. In this study, we further investigated the function of gga-miR-219b, and the gain/loss of function assay showed gga-miR-219b inhibited cell migration and reduced cell proliferation by promoting apoptosis not by cell cycle arrest. Gga-miR-219b also suppressed expression of two cell invasion-related genes MMP2 and MMP9. The results indicated suppressive effect of gga-miR-219b on MD tumorigenesis. The gene BCL11B was verified as a direct target gene of gga-miR-219b. RNA interference was performed to block BCL11B. As expected, the effects triggered by BCL11B downregulation were in accordance with that triggered by gga-miR-219b overexpression, suggesting that BCL11B was a stimulative regulator of MD transformation. Moreover, both gga-miR-219b and BCL11B influenced the expression of Meq gene, the most important oncogene in MDV. Additionally, gene expression level of anti-apoptotic genes BCL2 and BCL2L1 was downregulated and pro-apoptotic gene TNFSF10 was upregulated in MSB1 cells with gga-miR-219b overexpression or BCL11B knockdown, which suggested gga-miR-219b promoted cell apoptosis via regulating gene expression in the apoptosis pathways.

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Section: Introductionmentioning

confidence: 99%

Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1

Zhao

Han

et al. 2017

Sci Rep

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“…It was found that gga-miR-6651-5P , which is involved in regulating the process of resistance to the MADV [11], was the largest up-regulated in JCH compared to NH-P and its expression was only detected in JCH. We also noticed that gga-miR-122-5p was the largest down-regulated in JCH and it played an important role in the growth and development of liver, lipid metabolism [23].…”

Section: Resultsmentioning

confidence: 99%

“…As in detail, the top 8 miRNAs with higher expression in JCH (Table 2) were almost all involved in immunity and disease by analyzing the function of differential expressed miRNAs. For example, the gga-miRNA-6651-5p was only detected expression in JCH, and it had been demonstrated that it was dramatically increased in the MADV-GA-infected nontumorous samples but not in the tumorous and mock-infected groups, suggesting that it may be involved in regulating the process of resistance to the MADV [11]. Moreover, study had showed that miR-215 functions as a tumor suppressor that regulates cell cycle progression [38].…”

Section: Discussionmentioning

confidence: 99%

“…On the other aspect, miRNAs are involved in the regulation of the immune response. For example, 79 miRNAs that showed distinct expressions between marek’s disease virus infected-chicken splenic tumors and non-tumorous spleen tissues were identified by microarray [11]. Wang et al [12] demonstrated that gga-miRNA-21 can inhibit replication of infectious bursal virus through down-regulating of infectious bursal disease virus viral structural protein viral protein 1 at translation level [12].…”

Section: Introductionmentioning

confidence: 99%

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Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck

Luo

Liu

et al. 2016

Asian-Australas J Anim Sci

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ObjectiveThe bursa of Fabricius (BF) is a central humoral immune organ belonging specifically to avians. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two genetically disparate duck breeds.MethodsUsing Illumina next-generation sequencing, the miRNAs libraries of ducks were established.ResultsThe results showed that there were 66 differentially expressed miRNAs and 28 novel miRNAs in bursa. A set of abundant miRNAs (i.e., let-7, miR-146a-5p, miR-21-5p, miR-17~92) which are involved in immunity and disease were detected and the predicted target genes of the novel miRNAs were associated with duck high anti-adversity ability. By gene ontology analysis and enriching KEGG pathway, the targets of differential expressed miRNAs were mainly involved in immunity and disease, supporting that there were differences in the BF immune functions between the two duck breeds. In addition, the metabolic pathway had the maximum enriched target genes and some enriched pathways that were related to cell cycle, protein synthesis, cell proliferation and apoptosis. It indicted that the difference of metabolism may be one of the reasons leading the immune difference between the BF of two duck breeds.ConclusionThis data lists the main differences in the BF at miRNAs level between two genetically disparate duck breeds and lays a foundation to carry out molecular assisted breeding of poultry in the future.

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“…In chicken MD, Burnside et al [27] first identified the MDV-encoded and host-encoded miRNAs from MDV-infected chicken embryo fibroblast (CEF) cells. Over the last decade, many miRNAs encoded by the MD virus and chicken host have been discovered, and they are all thought to play critical roles in tumorigenesis [28][29][30][31][32][33][34][35][36][37][38][39][40][41]. The biological significance of the host miRNAs, however, needs to be further investigated.…”

Section: Introductionmentioning

confidence: 99%

Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek’s disease lymphoma cell proliferation and migration

Han

Lian

et al. 2016

Mol Biol Rep

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Marek's disease (MD) is an infectious disease of chickens caused by MD virus (MDV), which is a herpesvirus that initiates tumor formation. Studies have indicated that microRNAs (miRNAs) are linked with the development of cancers or tumors. Previously, gga-miR-130a was discovered downregulated in MDV-infected tissues. Here, we aimed to explore the further function of gga-miR-130a in MD. The expression of gga-miR-130a in MDV-infected and uninfected spleens was detected by quantitative real-time PCR (qRT-PCR). Subsequently, proliferation and migration assays of MDV-transformed lymphoid cells (MSB1) were carried out by transfecting gga-miR-130a. The target genes of gga-miR-130a were predicted using TargetScan and miRDB and clustered through Gene Ontology analysis. The target genes were validated by western blot, qRT-PCR, and a dual luciferase reporter assay. Our results show that the expression of gga-miR-130a was reduced in MDV-infected spleens. Gga-miR-130a showed an inhibitory effect on MSB1 cell proliferation and migration. Two target genes, homeobox A3 (HOXA3) and MyoD family inhibitor domain containing (MDFIC), were predicted and clustered to cell proliferation. Results indicate that gga-miR-130a regulates HOXA3 and MDFIC at the protein level but not at the mRNA level. Moreover, the gga-miR-130a binding sites of two target genes have been confirmed. We conclude that gga-miR-130a can arrest MSB1 cell proliferation and migration, and target HOXA3 and MDFIC, which are both involved in the regulation of cell proliferation. Collectively, gga-miR-130a plays a critical role in the tumorigenesis associated with chicken MD.

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Distinct expression pattern of miRNAs in Marek's disease virus infected-chicken splenic tumors and non-tumorous spleen tissues

Cited by 21 publications

References 28 publications

Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1

Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1

Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck

Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek’s disease lymphoma cell proliferation and migration

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