Distinct expression pattern of periostin splice variants in chondrocytes and ligament progenitor cells

Cai, Lei; Brophy, Robert H.; Tycksen, Eric; Duan, Xin; Nunley, Ryan M.; Farooq, Muhammad

doi:10.1096/fj.201802281r

Cited by 31 publications

(37 citation statements)

References 56 publications

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“…Third, periostin-DDR1 interaction results in AKT activation and increased β-catenin levels. We have previously reported that Postn activates Wnt/β-catenin signaling, and the Wnt signaling inhibitor CCT031374 hydrobromide, which blocks β-catenin stabilization and thus inhibits TCF-dependent transcription, abrogates Postn induction of MMP-13 expression in human chondrocytes [29]. In the present study, the DDR1 inhibitor 7rh also blocked Postn-induced MMP-13 upregulation, as well as AKT phosphorylation and β-catenin stabilization.…”

Section: Plos Onesupporting

confidence: 69%

“…Postn has the unique property of stimulating catabolic effects in cartilage while promoting osteogenesis in bone. Postn is produced by multiple joint tissues, including bone, cartilage, ligaments, meniscus, and synovium under normal or injury-induced conditions [29]. Karlsson et al and two other groups have independently shown increased expression of Postn in cartilage and subchondral bone of human OA-affected joints [3,4,[32][33][34]; and elevated levels of Postn have also been reported in human OA synovial fluids, as well as in synovial fluids of anterior cruciate ligament injury patients [35][36][37].…”

Section: Plos Onementioning

confidence: 99%

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Periostin interaction with discoidin domain receptor-1 (DDR1) promotes cartilage degeneration

et al. 2020

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Osteoarthritis (OA) is characterized by progressive loss of articular cartilage accompanied by the new bone formation and, often, a synovial proliferation that culminates in pain, loss of joint function, and disability. However, the cellular and molecular mechanisms of OA progression and the relative contributions of cartilage, bone, and synovium remain unclear. We recently found that the extracellular matrix (ECM) protein periostin (Postn, or osteoblastspecific factor, OSF-2) is expressed at high levels in human OA cartilage. Multiple groups have also reported elevated expression of Postn in several rodent models of OA. We have previously reported that in vitro Postn promotes collagen and proteoglycan degradation in human chondrocytes through AKT/β-catenin signaling and downstream activation of MMP-13 and ADAMTS4 expression. Here we show that Postn induces collagen and proteoglycan degradation in cartilage by signaling through discoidin domain receptor-1 (DDR1), a receptor tyrosine kinase. The genetic deficiency or pharmacological inhibition of DDR1 in mouse chondrocytes blocks Postn-induced MMP-13 expression. These data show that Postn is signaling though DDR1 is mechanistically involved in OA pathophysiology. Specific inhibitors of DDR1 may provide therapeutic opportunities to treat OA.

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Section: Plos Onesupporting

confidence: 69%

Section: Plos Onementioning

confidence: 99%

Periostin interaction with discoidin domain receptor-1 (DDR1) promotes cartilage degeneration

et al. 2020

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“…ACL remnants were removed from patients undergoing ACL reconstruction surgery. ACL progenitor cells that have been shown to express stem cell markers 30,31 were isolated as described before 32 . Moreover, we also isolated ACL fibroblasts as described previously 31 with slight modifications.…”

Section: Acl Sampling and Cell Preparationmentioning

confidence: 99%

Proteomic analysis of synovial fluid identifies periostin as a biomarker for anterior cruciate ligament injury

Brophy

Cai

Duan

et al. 2019

Osteoarthritis and Cartilage

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Objective: Emerging evidence suggests that injury to the anterior cruciate ligament (ACL) typically initiates biological changes that contribute to the development of osteoarthritis (OA). The molecular biomarkers or mediators of these biological events remain unknown. The goal of this exploratory study was to identify novel synovial fluid biomarkers associated with early biological changes following ACL injury distinct from findings in end-stage OA. Methods: Synovial fluid was aspirated from patients with acute (30 days) and subacute (31e90 days) ACL tears and from patients with advanced OA and probed via tandem mass spectrometry for biomarkers to distinguish OA from ACL injury. Periostin (POSTN) was identified as a potential candidate. Further analyses of POSTN were performed in synovial fluid, OA cartilage, torn ACL remnants, and cultured cells and media by Western blot, PCR, immunostaining and ELISA. Results: Synovial fluid analysis revealed that POSTN exhibited higher expression in subacute ACL injury than OA. POSTN expression was relatively low in cartilage/chondrocytes suggesting it is also produced by other intra-articular tissues. Conversely, high and time-dependent expression of POSTN in ACL tear remnants and isolated cells was consistent with the synovial fluid results. Conclusions: Elevated POSTN may provide a synovial fluid biomarker of subacute ACL injury setting separate from OA. Increased expression of POSTN in ACL suggests that the injured ACL may play a pivotal role in POSTN production, which is sensitive to time from injury. Previous studies have shown potential catabolic effects of POSTN, raising the possibility that POSTN contributes to the initiation of joint degeneration and may offer a window of opportunity to intervene in the early stages of post-traumatic OA.

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“…Originally, it was termed osteoblast-specific factor 2 (OSF-2) due to its localization in proximity to the periodontal ligament and periosteum of mice; it was renamed periostin in 1999 and subsequently classified as a matricellular protein in 2007 by Norris and Colleagues [ 18 ]. Structurally, periostin is a secreted 90 kDa N terminus-glycosylated protein with a multi-domain structure, including amino-terminal EMI domain, four tandem fascilin domains, and a carboxyl-terminal domain [ 19 ], with several different isoforms described [ 20 , 21 , 22 , 23 ]. This complex domain structure allows for numerous interactions with extracellular/secretory proteins [ 24 ], via which exerts its multiple functions.…”

Section: Gla-containing Ecm Proteinsmentioning

confidence: 99%

Functions of Matricellular Proteins in Dental Tissues and Their Emerging Roles in Orofacial Tissue Development, Maintenance, and Disease

Nikoloudaki

2021

IJMS

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Matricellular proteins (MCPs) are defined as extracellular matrix (ECM) associated proteins that are important regulators and integrators of microenvironmental signals, contributing to the dynamic nature of ECM signalling. There is a growing understanding of the role of matricellular proteins in cellular processes governing tissue development as well as in disease pathogenesis. In this review, the expression and functions of different MP family members (periostin, CCNs, TSPs, SIBLINGs and others) are presented, specifically in relation to craniofacial development and the maintenance of orofacial tissues, including bone, gingiva, oral mucosa, palate and the dental pulp. As will be discussed, each MP family member has been shown to have non-redundant roles in development, tissue homeostasis, wound healing, pathology and tumorigenesis of orofacial and dental tissues.

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Distinct expression pattern of periostin splice variants in chondrocytes and ligament progenitor cells

Cited by 31 publications

References 56 publications

Periostin interaction with discoidin domain receptor-1 (DDR1) promotes cartilage degeneration

Periostin interaction with discoidin domain receptor-1 (DDR1) promotes cartilage degeneration

Proteomic analysis of synovial fluid identifies periostin as a biomarker for anterior cruciate ligament injury

Functions of Matricellular Proteins in Dental Tissues and Their Emerging Roles in Orofacial Tissue Development, Maintenance, and Disease

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