Distinct growth and extractive methods of Acmellaoleracea (L.) R.K. Jansen rising different concentrations of spilanthol: An important bioactive compound in human dietary

França, Júlia V.; Queiroz, Maria Sandra Ramos; Amaral, Bruna Paes do; Simas, Naomi Kato; Silva, Nina Claudia Barboza da; Leal, Ivana Corrêa Ramos

doi:10.1016/j.foodres.2016.09.018

Cited by 17 publications

(8 citation statements)

References 42 publications

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“…The microtiter plates were incubated for additional 4 h at 37 • C in order to observe pH alterations in the medium, such as the color changing from blue to pink in the presence of bacterial growth. The experiment was followed by optical density determination by a microplate reader spectrophotometer at a wavelength of 570 nm [29] in triplicate.…”

Section: Identification Of Np-protein Conjugation By Immunogoldmentioning

confidence: 99%

Vancomycin-loaded nanoparticles against vancomycin intermediate and methicillin resistant Staphylococcus aureus strains

et al. 2020

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Bacterial infections represent one of the leading causes of mortality in the world. Among causative pathogens, S. aureus is prominently known as the underlying cause of many multidrug resistant infections that are often treated with the first-line choice antibiotic vancomycin (VCM). Loading antibiotics into polymeric nanoparticles (Np) displays promise as an alternative method to deliver therapy due to the greater access and accumulation of the antibiotic at the site of the infection as well as reducing toxicity, irritation and degradation. The aim of this work was to prepare, characterize and evaluate VCM-loaded nanoparticles (VNp) for use against S. aureus strains. Moreover, conjugation of Nps with holo-transferrin (h-Tf) was investigated as an approach for improving targeted drug delivery. VNp were prepared by double emulsion solvent evaporation method using PLGA and PVA or DMAB as surfactants. The particles were characterized for size distribution, Zeta Potential, morphology by transmission electron microscopy, encapsulation yield and protein conjugation efficiency. Process yield and drug loading were also investigated along with an in vitro evaluation of VNp antimicrobial effects against S. aureus strains. Results showed that Np were spontaneously formed with a mean diameter lower than 300 nm in a narrow size distribution that presented a spherical shape. The bioconjugation with h-Tf did not appear to increase the antimicrobial effect of VNp. However, non-bioconjugated Np presented a minimal inhibitory concentration lower than free VCM against a MRSA (Methicillin-resistant S. aureus) strain, and slightly higher against a 6 Author to whom any correspondence should be addressed.

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Section: Identification Of Np-protein Conjugation By Immunogoldmentioning

confidence: 99%

Vancomycin-loaded nanoparticles against vancomycin intermediate and methicillin resistant Staphylococcus aureus strains

et al. 2020

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Section: Discussionmentioning

confidence: 99%

“…On the other hand, regarding the screening of extraction techniques, earlier research reported the use of microwave-assisted extraction (Franca et al 2016 ), supercritical CO 2 extraction (Dias et al 2012 ), and Soxhlet (Bakondi et al 2019 ). For instance, Franca et al ( 2016 ) compared microwave-assisted extraction with normal maceration, demonstrating that the use of microwave allowed obtaining the highest amount of spilanthol. Bellumori et al ( 2022 ) recently screened three different A. oleracea extraction procedures using ethanol 80% v/v as extractive solvent:sonication at 60 °C for 10 min, magnetic stirring for 50 min followed by sonication for 10 min, and sonication for 10 min at room temperature.…”

Section: Discussionmentioning

confidence: 99%

Acmella oleracea extracts as green pesticides against eight arthropods attacking stored products

Kavallieratos,

Spinozzi,

Filintas

et al. 2023

Environ Sci Pollut Res

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Developing sustainable control tools for managing noxious pests attacking stored foodstuffs is a timely research challenge. Acmella oleracea (L.) R. K. Jansen is a crop widely cultivated for its multiple usages on an industrial level. In this study, the extracts prepared with A. oleracea aerial parts were applied on wheat kernels for the management of eight important arthropod pests attacking stored products, i.e., Cryptolestes ferrugineus, Tenebrio molitor, Oryzaephilus surinamensis, Trogoderma granarium, Tribolium castaneum, Tribolium confusum, Alphitobius diaperinus (adults/larvae), and Acarus siro (adults/nymphs). Extraction of A. oleracea was optimized on the base of the yield and content of spilanthol and other N-alkylamides which were analysed by HPLC–DAD-MS. Two concentrations of n-hexane or methanol extracts (500 ppm and 1000 ppm), obtained through Soxhlet extraction, were tested to acquire mortality data on the above-mentioned pests after 4, 8, and 16 h and 1 to 7 days of exposure. Both extracts achieved complete mortality (100.0%) of C. ferrugineus adults. In the case of A. diaperinus adults, mortalities were very low at any concentrations of both extracts. In general, the n-hexane extract was more efficient than methanol extract against almost all species and stages. Considering both extracts, the susceptibility order, from most to least susceptible species/stage, was C. ferrugineus adults > A. diaperinus larvae > C. ferrugineus larvae > T. granarium adults > T. molitor larvae > O. surinamensis adults > O. surinamensis larvae > T. confusum larvae > T. castaneum larvae > A. siro adults > T. molitor adults > A. siro nymphs > T. granarium larvae > T. castaneum adults > T. confusum adults > A. diaperinus adults. Our research provides useful knowledge on the efficacy of N-alkylamides-rich A. oleracea extracts as grain protectants, pointing out the importance of targeting the most susceptible species/ developmental stages.

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“…A few years later, Franca et al evaluated spilanthol content in A. oleracea plants obtained from three different cultivation conditions: in vitro, acclimatized and in-field and compared two different extraction techniques, such as maceration and microwave-assisted extraction (MAE). Results showed that the acclimatized plants were the richest in spilanthol, followed by the in vitro culture and, finally, the field material [ 48 ]. The aim of another study was to determine the best concentration of growth regulators for the in vitro cultivation of jambù and to elaborate an efficient micropropagation protocol.…”

Section: Cultivation and Micropropagationmentioning

confidence: 99%

A Review of the Chemistry and Biological Activities of Acmella oleracea (“jambù”, Asteraceae), with a View to the Development of Bioinsecticides and Acaricides

Ferrati

Baldassarri

Cappellacci

et al. 2022

Plants

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Human pathologies, environmental pollution, and resistance phenomena caused by the intensive use of chemical pesticides have shifted the attention of the agrochemical industries towards eco-friendly insecticides and acaricides. Acmella oleracea (L.) R. K. Jansen (jambù) is a plant native to South America, widely distributed and cultivated in many countries due to its numerous pharmacological properties. This review analyzes literature about the plant, its uses, and current knowledge regarding insecticidal and acaricidal activity. Acmella oleracea has proven to be a potential pesticide candidate against several key arthropod pest and vector species. This property is inherent to its essential oil and plant extract, which contain spilanthol, the main representative of N-alkylamides. As a result, there is a scientific basis for the industrial exploitation of jambù in the preparation of green insecticides. However, studies related to its toxicity towards non-target species and those aimed at formulating and developing marketable products are lacking.

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Distinct growth and extractive methods of Acmellaoleracea (L.) R.K. Jansen rising different concentrations of spilanthol: An important bioactive compound in human dietary

Cited by 17 publications

References 42 publications

Vancomycin-loaded nanoparticles against vancomycin intermediate and methicillin resistant Staphylococcus aureus strains

Vancomycin-loaded nanoparticles against vancomycin intermediate and methicillin resistant Staphylococcus aureus strains

Acmella oleracea extracts as green pesticides against eight arthropods attacking stored products

A Review of the Chemistry and Biological Activities of Acmella oleracea (“jambù”, Asteraceae), with a View to the Development of Bioinsecticides and Acaricides

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