Distinct hemogenic potential of endothelial cells and CD41⁺ cells in mouse embryos

Abstract

Definitive hematopoietic progenitor cells have been thought to develop from the vascular endothelium located in the aorta-gonad-mesonephros region of the mouse embryo. However, several recent findings have suggested that most hematopoietic progenitors are derived from non-endothelial precursor cells expressing CD41. We characterized two distinct precursor populations of definitive hematopoietic cell lineages, vascular endothelial (VE)-cadherin + CD41 -CD45 -endothelial cells and CD41 + CD45 -non-endothelial progenitors, both of which are derived from lateral mesoderm. VE-cadherin + endothelial cells obtained from cultures of differentiating embryonic stem cells possessed hematopoietic potential encompassing erythroid, myeloid and B lymphoid lineages, whereas CD41 + progenitors lacked the B lymphopoietic potential. VE-cadherin + endothelial cells in the lower trunk of the embryo proper showed a significant potential for initiating B lymphopoiesis in cultures, while endothelial cells in the yolk sac appeared to have a bias for myeloerythropoietic differentiation. CD41 + progenitors isolated from yolk sac and embryo proper were capable of generating multiple hematopoietic lineages, although mast cell precursors were exclusively enriched in CD41 + progenitors in the yolk sac. These results suggest that hemogenic endothelial cells and CD41 + progenitors possess distinct hematopoietic potential depending on the tissues in which they reside.

Role of Central Leptin Signaling in the Starvation-Induced Alteration of B-Cell Development

“…Flow cytometry analysis was performed to evaluate the B lineage cells in the bone marrow, mononuclear cells in the circulating blood, and splenocytes as described (Hashimoto et al, 2007;Kim-Saijo et al, 2008) using a FACSCalibur HG (BD Biosciences). The data were analyzed with BD CellQuest Pro (BD Biosciences).…”

Embryonic origin of the adult hematopoietic system: advances and questions

“…Since CD41 marks megakaryocytes and all hematopoietic progenitors in the early embryo (Bertrand et al, 2005;Corbel and Salaun, 2002;Ferkowicz et al, 2003;Mikkola et al, 2003), these cells cannot be considered to be endothelial either. Although some CD41 cells express VE-cadherin, they lack endothelial potential (Hashimoto et al, 2007;Li et al, 2005). The role of VE-cadherin in pre-dHSCs might be in facilitating interactions with endothelial cells and mediating essential signaling (Carmeliet et al, 1999).…”

Identification of a yolk sac cell population with hematopoietic activity in view of CD45/c-Kit expression

“…The YS cells were filtered through a 30-lm mesh (Cell Trics; Perteck, Mü nster, Germany), and stained with phycoerythrin (PE)-conjugated anti-mouse CD45 (30-F11; eBioscience, San Diego, CA, USA) and allophycocyanin (APC)-conjugated anti-mouse c-Kit (2B8; eBioscience) antibodies. The YS cells were also stained with fluorescein isothiocyanate-conjugated anti-mouse CD34 (RAM34; eBioscience), anti-mouse Mac-1 (M1 ⁄ 70; eBioscience), anti-mouse vascular endothelial-cadherin (VECD1) (Matsuyoshi et al 1997;Hashimoto et al 2007) …”