Distinct nodule and leaf functions of two different sucrose phosphate synthases in alfalfa

Padhi, Shanta; Grimes, Martha M.; Muro-Villanueva, Fabiola; Ortega, J. L. Araus; Sengupta‐Gopalan, Champa

doi:10.1007/s00425-019-03261-9

Cited by 8 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The interesting finding was that members of family A ( GmSPS13 / 17 ) were significantly expressed at branching stage, flowering stage, and fruiting stage, the critical period of symbiotic nitrogen fixation. As the ortholog gene of GmSPS13 / 17 in alfalfa, SPSA also showed nodule-enhanced expression [ 40 ] and was involved in the synthesis of sucrose in nodules [ 51 ]. These results indicated that legume SPS members of family A may have the conserved function in carbon metabolism in nodules.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of GmSPS Gene Family in Soybean and Expression Analysis in Response to Cold Stress

Shen,

Xu,

Yuan

et al. 2023

IJMS

View full text Add to dashboard Cite

Sucrose metabolism plays a critical role in development, stress response, and yield formation of plants. Sucrose phosphate synthase (SPS) is the key rate-limiting enzyme in the sucrose synthesis pathway. To date, genome-wide survey and comprehensive analysis of the SPS gene family in soybean (Glycine max) have yet to be performed. In this study, seven genes encoding SPS were identified in soybean genome. The structural characteristics, phylogenetics, tissue expression patterns, and cold stress response of these GmSPSs were investigated. A comparative phylogenetic analysis of SPS proteins in soybean, Medicago truncatula, Medicago sativa, Lotus japonicus, Arabidopsis, and rice revealed four families. GmSPSs were clustered into three families from A to C, and have undergone five segmental duplication events under purifying selection. All GmSPS genes had various expression patterns in different tissues, and family A members GmSPS13/17 were highly expressed in nodules. Remarkably, all GmSPS promoters contain multiple low-temperature-responsive elements such as potential binding sites of inducer of CBF expression 1 (ICE1), the central regulator in cold response. qRT-PCR proved that these GmSPS genes, especially GmSPS8/18, were induced by cold treatment in soybean leaves, and the expression pattern of GmICE1 under cold treatment was similar to that of GmSPS8/18. Further transient expression analysis in Nicotiana benthamiana and electrophoretic mobility shift assay (EMSA) indicated that GmSPS8 and GmSPS18 transcriptions were directly activated by GmICE1. Taken together, our findings may aid in future efforts to clarify the potential roles of GmSPS genes in response to cold stress in soybean.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of GmSPS Gene Family in Soybean and Expression Analysis in Response to Cold Stress

Shen,

Xu,

Yuan

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…The role of SPS and Suc biosynthesis in mutualistic interactions has been substantially studied in the rhizobium-legume symbiosis between Medicago sativa and Sinorhizobium meliloti [77,78]. Three genes encode the different SPS isoforms in M. sativa MsSPSA, MsSPSB, and MsSPSB3.…”

Section: Plant Sps Isoforms and Their Role In Sucrose Biosynthesis Ti...mentioning

confidence: 99%

“…The study of SPS, SuSy, and SPP in WT and Fix − mutant nodules suggested that SPS activity plays an important role in the physiology of the nodule and in the mobilization of carbon towards the symbiotic microorganism [77]. Further studies in M. sativa defined that SPSB controls sucrose biosynthesis in leaves, while SPSA is crucial for the maintenance of the regulatory cycles of sucrose biosynthesis/breakdown from sucrose and starch catabolic products [78]. These results suggest that SPS could be involved in the modulation of the C amount provided to the symbiont through the Suc synthesis in the nodules, and that the nodule-induced SPS gene expression from a particular phylogenetic family is specifically regulated in response to the interaction of the plant with mutualistic microorganisms [77,78].…”

Section: Plant Sps Isoforms and Their Role In Sucrose Biosynthesis Ti...mentioning

confidence: 99%

“…Further studies in M. sativa defined that SPSB controls sucrose biosynthesis in leaves, while SPSA is crucial for the maintenance of the regulatory cycles of sucrose biosynthesis/breakdown from sucrose and starch catabolic products [78]. These results suggest that SPS could be involved in the modulation of the C amount provided to the symbiont through the Suc synthesis in the nodules, and that the nodule-induced SPS gene expression from a particular phylogenetic family is specifically regulated in response to the interaction of the plant with mutualistic microorganisms [77,78]. Suc transported to the nodules is primarily catabolized in the vascular tissues, and the hexoses obtained are then transported to the central part of the nodules, where they are routed to starch biosynthesis.…”

Section: Plant Sps Isoforms and Their Role In Sucrose Biosynthesis Ti...mentioning

confidence: 99%

See 1 more Smart Citation

An Updated Review on the Modulation of Carbon Partitioning and Allocation in Arbuscular Mycorrhizal Plants

et al. 2021

View full text Add to dashboard Cite

Arbuscular mycorrhizal fungi (AMF) are obligate biotrophs that supply mineral nutrients to the host plant in exchange for carbon derived from photosynthesis. Sucrose is the end-product of photosynthesis and the main compound used by plants to translocate photosynthates to non-photosynthetic tissues. AMF alter carbon distribution in plants by modifying the expression and activity of key enzymes of sucrose biosynthesis, transport, and/or catabolism. Since sucrose is essential for the maintenance of all metabolic and physiological processes, the modifications addressed by AMF can significantly affect plant development and stress responses. AMF also modulate plant lipid biosynthesis to acquire storage reserves, generate biomass, and fulfill its life cycle. In this review we address the most relevant aspects of the influence of AMF on sucrose and lipid metabolism in plants, including its effects on sucrose biosynthesis both in photosynthetic and heterotrophic tissues, and the influence of sucrose on lipid biosynthesis in the context of the symbiosis. We present a hypothetical model of carbon partitioning between plants and AMF in which the coordinated action of sucrose biosynthesis, transport, and catabolism plays a role in the generation of hexose gradients to supply carbon to AMF, and to control the amount of carbon assigned to the fungus.

show abstract

Underlying mechanism on source-sink carbon balance of grazed perennial grass during regrowth: Insights into optimal grazing regimes of restoration of degraded grasslands in a temperate steppe

Zhang

Gong²,

Li³

et al. 2021

Journal of Environmental Management

View full text Add to dashboard Cite

Distinct nodule and leaf functions of two different sucrose phosphate synthases in alfalfa

Cited by 8 publications

References 51 publications

Genome-Wide Identification of GmSPS Gene Family in Soybean and Expression Analysis in Response to Cold Stress

Genome-Wide Identification of GmSPS Gene Family in Soybean and Expression Analysis in Response to Cold Stress

An Updated Review on the Modulation of Carbon Partitioning and Allocation in Arbuscular Mycorrhizal Plants

Underlying mechanism on source-sink carbon balance of grazed perennial grass during regrowth: Insights into optimal grazing regimes of restoration of degraded grasslands in a temperate steppe

Contact Info

Product

Resources

About