Distinct Peripheral Blood RNA Responses to Salmonella in Pigs Differing in Salmonella Shedding Levels: Intersection of IFNG, TLR and miRNA Pathways

Huang, Ting Hua; Uthe, Jolita J.; Bearson, Shawn M.D.; Demirkale, Cumhur Yusuf; Nettleton, Dan; Knetter, Susan M.; Christian, Curtis; Ramer‐Tait, Amanda E.; Wannemuehler, Michael J.; Tuggle, Christopher K.

doi:10.1371/journal.pone.0028768

Cited by 51 publications

(88 citation statements)

References 96 publications

(127 reference statements)

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“…[96,97]), while studies in the pig or cow disproportionately revolve around resistance to infection, in particular Mycobacterium bovis in cattle and Salmonella in pigs (e.g. [98][99][100]). Reproductive biology studies are also well represented (e.g.…”

Section: Blood Transcriptome Profiling Studies In Health and Diseasementioning

confidence: 99%

Assessment of immune status using blood transcriptomics and potential implications for global health

Chaussabel¹

2015

Seminars in Immunology

103

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The immune system plays a key role in health maintenance and pathogenesis of a wide range of diseases. Leukocytes that are present in the blood convey valuable information about the status of the immune system. Blood transcriptomics, which consists in profiling blood transcript abundance on genome-wide scales, has gained in popularity over the past several years. Indeed, practicality and simplicity largely makes up for what this approach may lack in terms of cell population-level resolution. An extensive survey of the literature reveals increasingly widespread use across virtually all fields of medicine as well as across a number of different animal species, including model organisms but also animals of economical importance. Dissemination across such a wide range of disciplines holds the promise of adding a new perspective, breadth or context, to the considerable depth afforded by whole genome profiling of blood transcript abundance. Indeed, it is only through such contextualization that a truly global perspective will be gained from the use of systems approaches. Also discussed are opportunities that may arise for the fields of immunology and medicine from using blood transcriptomics as a common denominator for developing interactions and cooperation across fields of research that have traditionally been and largely remain compartmentalized. Finally, an argument is made for building immunology research capacity using blood transcriptomics platforms in low-resource and high-disease burden settings.

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Section: Blood Transcriptome Profiling Studies In Health and Diseasementioning

confidence: 99%

Assessment of immune status using blood transcriptomics and potential implications for global health

Chaussabel¹

2015

Seminars in Immunology

103

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“…Six piglets (one from each sow) were housed separately from ST-inoculated piglets, and were inoculated with PBS alone, as described previously. 15 Data were collected at 0, 1, 2, 7, 14 and 21 DPI. Bacteriology for qualitative and quantitative analysis of Salmonella from rectal swabs was performed as described previously.…”

Section: Animalsmentioning

confidence: 99%

“…Further, we have previously determined that there is little difference in gene expression in peripheral blood sampled at 20 DPI for LS and PS pigs. 15 RNA was purified by DNase I digestion and the RNeasy Mini Elute Cleanup Kit as recommended by the manufacturer (Qiagen). RNA samples were confirmed to be DNA-free by lack of genomic DNA on PCR amplification.…”

Section: Rna Preparation Microarray Hybridization and Quantitative Pcrmentioning

confidence: 99%

Salmonella entericaserovar Typhimurium-infected pigs with different shedding levels exhibit distinct clinical, peripheral cytokine and transcriptomic immune response phenotypes

et al. 2014

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Christopher, "Salmonella enterica serovar Typhimurium-infected pigs with different shedding levels exhibit distinct clinical, peripheral cytokine and transcriptomic immune response phenotypes" (2014 AbstractFoodborne salmonellosis costs the US $2.7 billion/year, including $100.0 million in annual losses to pork producers. Pigs colonized with Salmonella are usually asymptomatic with varied severity and duration of fecal shedding. Thus, understanding the responses that result in less shedding may provide a mechanism for control. Fifty-four pigs were inoculated with Salmonella enterica serovar Typhimurium (ST) and clinical signs, fecal ST shedding, growth performance, peripheral cytokines and whole blood gene expression were measured. Persistently shedding (PS) pigs had longer pyrexia and elevated serum IL-1b, TNF-a and IFN-g compared with low shedding (LS) pigs, while LS pigs had brief pyrexia, less shedding that decreased more rapidly and greater serum CXCL8 than PS pigs. The PS pigs up-regulated genes involved with the STAT1, IFNB1 and IFN-g networks on d 2, while up-regulation of genes involved in immune response regulation were only detected in LS pigs. This is the first study to examine host responses to ST infection at a clinical, performance, cytokine and transcriptomic level. The results indicated that pigs with different shedding outcomes developed distinct immune responses within the first 2 d of ST infection, and elucidated alternative mechanisms that could be targeted to reduce Salmonella shedding and spread.

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“…2 of 18 In addition, TLRs also react to damage associated molecular patterns (DAMPs) [2,3] released after cellular damage, and thus play a crucial role in initiating the innate immune response [4]. Genetic variations found in the genes encoding TLRs have been associated with disease susceptibility and resistance in a variety of animal species [1,5,6]. In the bovine genome, 10 members of the TLR family (TLR1-10) have been identified and mapped to specific chromosomes [7].…”

Section: Introductionmentioning

confidence: 99%

Determination of Genetic Variations of Toll-like Receptor (TLR) 2, 4 and 6 with Next Generation Sequencing in Native Cattle Breeds of Anatolia and Holstein Friesian

Bilgen¹,

Kul²,

Offord³

et al. 2016

Preprint

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Abstract:In recent years, the focus of disease resistance and susceptibility studies in cattle have been on determining patterns in the innate immune response of key proteins, such as Toll-like receptors (TLR). In the bovine genome, there are 10 TLR family members and, of these, TLR2, TLR4 and TLR6 are specialized in recognition of bacterial ligands. Indigenous cattle breeds of Anatolia have been reported to show fewer signs of clinical bacterial infections, such as bovine tuberculosis and mastitis, and it is hypothesized that this might be due to a less stringent genetic selection during breeding.In contrast, Holstein-Friesian cattle have been under strong selection for milk production, which may have resulted in greater susceptibility to diseases. To test this hypothesis, we have compared the TLR2, TLR4 and TLR6 genes of Anatolian Black (AB), East Anatolian Red (EAR), South Anatolian Red (SAR), Turkish Grey (TG), and Holstein (HOL) cattle using Next Generation Sequencing. The SAR breed had the most variations overall, followed by EAR, AB, TG and HOL. TG had the most variations for TLR2 whereas SAR had the most variations in TLR4 and TLR6. We compared these variants with those associated with disease and susceptibility traits. We used exon variants to construct haplotypes, investigated shared haplotypes within breeds and determined candidate haplotypes for disease resistance phenotype in Anatolian cattle breeds.

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Distinct Peripheral Blood RNA Responses to Salmonella in Pigs Differing in Salmonella Shedding Levels: Intersection of IFNG, TLR and miRNA Pathways

Cited by 51 publications

References 96 publications

Assessment of immune status using blood transcriptomics and potential implications for global health

Assessment of immune status using blood transcriptomics and potential implications for global health

Salmonella entericaserovar Typhimurium-infected pigs with different shedding levels exhibit distinct clinical, peripheral cytokine and transcriptomic immune response phenotypes

Determination of Genetic Variations of Toll-like Receptor (TLR) 2, 4 and 6 with Next Generation Sequencing in Native Cattle Breeds of Anatolia and Holstein Friesian

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