Distinct replicative and cytopathic characteristics of human immunodeficiency virus isolates

Fenyõ, Éva Mária; Morfeldt-Månson, Linda; Chiodi, Francesca; Lind, Birgitta; Gegerfelt, Agneta von; Albert, Jan; Olausson, Eric; Åsjö, Birgitta

doi:10.1128/jvi.62.11.4414-4419.1988

Cited by 410 publications

(151 citation statements)

References 22 publications

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“…Virus was isolated from PBMC as described [14]. It was characterized as being of the slow/low, non syncytia-inducing (NSI) or rapid/high, syncytia-inducing (SI) phenotype by cocultivating virus-positive lymphocytes with cells of the MT-2, U-937 and Jurkat-tat lines [15].…”

Section: Hiv Isolation and Quantificationmentioning

confidence: 99%

Dynamics of HIV-1 replication following influenza vaccination of HIV+ individuals

Røsok¹,

Voltersvik²,

Bjerknes³

et al. 1996

Clinical and Experimental Immunology

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Levels of HIV‐1 have been reported to increase in peripheral blood after influenza vaccination of HIV+ individuals. In this study we have evaluated the dynamics of these changes. Ten HIV‐1+ individuals classified in revised CDC clinical categories B and C as well as five seronegative healthy controls were vaccinated with the recommended influenza strains. HIV viral RNA and proviral DNA were sequentially quantified in serum and blood lymphocytes, respectively. Nine of the 10 HIV+ individuals had an increase in the frequency of infected CD4 cells 2 weeks after influenza vaccination. Individuals with low viral load had a rapid increase in viraemia and a small increase in frequency of infected cells in peripheral blood. In contrast, individuals with high viral load had a small drop in viraemia followed by a significant rise in the rate of infected cells. The observed changes may resemble those taking place during intercurrent infections in HIV+ individuals. The effects of the relative increases in infectious virus after the transient viraemic phase should be further investigated to evaluate potential risks of vaccination.

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Section: Hiv Isolation and Quantificationmentioning

confidence: 99%

Dynamics of HIV-1 replication following influenza vaccination of HIV+ individuals

Røsok¹,

Voltersvik²,

Bjerknes³

et al. 1996

Clinical and Experimental Immunology

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“…syncytium formation has been shown to be associated with an increase in cellular oleic acid (Apostolov et al, 1989) indicating an increase in membrane fluidity. Mounting evidence suggests that virulent HIV isolates from immunodeficient individuals expressing AIDS possess a greater syncytium-forming CPE than those from earlier stages of the ARC and AIDS (Cheng-Mayer et al, 1988;Fenyo et al, 1988, Fiore et al, 1990Tersmette et al, 1988Tersmette et al, , 1989. The rapid evolution of HIV in vivo appears to continually generate subpopulations of virions exhibiting an increasing cytopathic potential throughout the progression of AIDS (Habeshaw et al, 1990).…”

Section: B the Possible Significance Of T-lymphocyte Depletion In Aimentioning

confidence: 99%

The ultrastructure of multinucleate giant cells

Harris

1993

Micron

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A survey of the available ultrastructural data on physiologically and pathologically occurring and virally-induced multinucleate giant cells (MNGCs) is presented. Emphasis is initially placed upon the bone osteoclast, the skeletal muscle myotube and the placental syncytiotrophoblast. The widespread occurrence of MNGCs in a range of pathological situations is discussed, with emphasis upon the broad involvement of the macrophage in inflammatory responses. Many viruses produce cell fusion in vivo and in vitro when cell cultures are infected. Several examples are given. A clear distinction is drawn between viral fusion from "without" and viral fusion from "within" the cell. The cytopathic effect (CPE) of the animal and human retroviruses is discussed in considerable detail. The in vivo and in vitro formation of lymphocytic and macrophage MNGCs by HIV-1 is given extensive coverage. The possible significance of the presence of brain MNGCs of macrophage/ microglial origin as a cellular feature of AIDS dementia is discussed. A new hypothesis is advanced relating to the possible role of endogenous C-type retrovirus in the physiological fusion of the invasive placental cytotrophoblasts to create the syncytiotrophoblast. The evolutionary and developmental significance of such an event in relation to the evolution of the placental mammals is discussed. The possible importance of MNGC formation in the depletion of the CD4 ÷ population of T-lymphocytes in vivo in the clinical progression of the AIDS-related complex and AIDS is related to the potent fusogenic effect of HIV-I when cell cultures are infected.

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“…Fouchier et al, manuscript in preparation). Although reports that describe syncytia in cells from HIV-infected individuals are scarce, SI variants may induce cell death in syncytium-forming cells in vivo [14,24,25]. Secondly, the high fusion capacity of SI HIV-1 isolates was found to be associated with a broad T-cell host range and high replication rate in vitro (R.A.M.…”

Section: Si Capacitymentioning

confidence: 99%

Molecular determinants of human immunodeficiency virus type I phenotype variability

Fouchier

Schuitemaker

1996

Eur J Clin Investigation

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Abstract.The clinical course of HIV-1-infected individuals can be highly variable. Progression to AIDS can occur within 1 year after infection but symptom-free infection for more than 15 years has also been reported. This variation can either be determined by host factors, the biological variability of the virus or both. Here the molecular determinants and clinical relevance of HIV-1 biological phenotype variability are reviewed.

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Distinct replicative and cytopathic characteristics of human immunodeficiency virus isolates

Cited by 410 publications

References 22 publications

Dynamics of HIV-1 replication following influenza vaccination of HIV+ individuals

Dynamics of HIV-1 replication following influenza vaccination of HIV+ individuals

The ultrastructure of multinucleate giant cells

Molecular determinants of human immunodeficiency virus type I phenotype variability

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