Distinction between breast cancer cell subtypes using third harmonic generation microscopy

Gavgiotaki, Evangelia; Filippidis, George; Markomanolaki, Haris; Kenanakis, George; Agelaki, Sofia; Georgoulias, Vassilis; Athanassakis, Irene

doi:10.1002/jbio.201600173

Cited by 28 publications

(39 citation statements)

References 54 publications

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“…Non-linear microscopy (NLM) diagnostic modalities are also included in Table 1. Non-linear microscopy techniques (NLM), such as MPEF, second harmonic generation (SHG), and third harmonic generation (THG), are well-established techniques for biological research [109][110][111][112][113][114]. These techniques under different modalities provide new insights for the assessment of appropriate conservation methods that have to be applied for various CH objects of high artistic or cultural significance [79,115].…”

Section: Non-linear Microscopymentioning

confidence: 99%

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Non-Linear Microscopy: A Well-Established Technique for Biological Applications towards Serving as a Diagnostic Tool for in situ Cultural Heritage Studies

Mari

Filippidis

2020

Sustainability

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A range of sophisticated imaging techniques have been developed in recent years that can reveal the surface structure of cultural heritage objects with varying precision. In combination with various spectroscopic methods, they allow the study of the chemical composition of the object; thus, conclusions can be drawn about the origin of the object or its initial components, method, or time of creation, authenticity, mechanisms of degradation, and ways of further conservation. At present, different techniques can be applied to a wide range of cultural heritage objects, such as varnishes, paintings, archaeological objects, binding media, paper-based documents, parchments, marbles, frescoes, as well as various objects made of leather, fabric, stone, ceramics and glass, wood, or metal. One of the main needs in the study of cultural heritage (CH) is the transportability/portability of the research equipment, since many pieces under investigation cannot be moved to the laboratory, either because of their size, inseparability (for example, frescoes on walls, mural paintings in caves), or the threat of damage. In this work, we briefly overview the main optical- and laser-based methods used for the study of cultural heritage objects indicating the scope of their application, and we focus on the applications of non-linear microscopic methods for the investigation of a series of artifacts. We also discuss all the requirements for the construction of a prototype transportable non-linear optical system that will be used as a novel diagnostic tool for in situ studies of CH assets. The availability of such a transportable workstation will significantly improve the study and characterization of various types of CH objects and will constitute an extremely useful diagnostic tool for heritage scientists dealing with a variety of investigations.

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Section: Non-linear Microscopymentioning

confidence: 99%

“…SHG enables the detection of high degree-organized structures without inversion symmetry, such as stacked membranes and arranged proteins (e.g., collagen) [80,111]. THG allows the resolution of transparent interfaces, on the basis of local differences in refractive index and dispersion [79,110,112].…”

Section: Second and Third Harmonic Generation (Shg Thg)mentioning

confidence: 99%

Non-Linear Microscopy: A Well-Established Technique for Biological Applications towards Serving as a Diagnostic Tool for in situ Cultural Heritage Studies

Mari

Filippidis

2020

Sustainability

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“…More important, THG microscopy has shown great potential for clinical applications. Excellent agreement with the standard histopathology of skin cancers has been demonstrated for THG [10,11] and THG also shows great potential for breast tumor diagnosis [12,13].…”

Section: Introductionmentioning

confidence: 98%

Tensor regularized total variation for denoising of third harmonic generation images of brain tumors

Zhang

Groot

Munck

2018

Journal of Biophotonics

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Third harmonic generation (THG) microscopy shows great potential for instant pathology of brain tissue during surgery. However, the rich morphologies contained and the noise associated makes image restoration, necessary for quantification of the THG images, challenging. Anisotropic diffusion filtering (ADF) has been recently applied to restore THG images of normal brain, but ADF is hard-to-code, time-consuming and only reconstructs salient edges. This work overcomes these drawbacks by expressing ADF as a tensor regularized total variation model, which uses the Huber penalty and the L norm for tensor regularization and fidelity measurement, respectively. The diffusion tensor is constructed from the structure tensor of ADF yet the tensor decomposition is performed only in the non-flat areas. The resulting model is solved by an efficient and easy-to-code primal-dual algorithm. Tests on THG brain tumor images show that the proposed model has comparable denoising performance as ADF while it much better restores weak edges and it is up to 60% more time efficient.

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“…More studies are needed to improve the therapeutic interventions for the treatment of human breast cancers [12][13][14]. Different subtypes of breast-cancer cells vary with respect to prognosis, aggressiveness, and response to therapies.…”

Section: Introductionmentioning

confidence: 99%

Resveratrol Modifies Lipid Composition of Two Cancer Cell Lines

Gomes

Viana

Silva

et al. 2020

BioMed Research International

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Resveratrol (Resv) offers health benefits in cancer and has been reported to modulate important enzymes of lipid metabolism. Studies of its effects on lipid composition in different subtypes of breast-cancer cells are scarce. Thus, we investigated the alterations in phospholipids (PL), fatty acids (FA), and lipid metabolism enzymes in two breast-cancer cell lines after Resv treatment. MCF-7 and MDA-MB-231 cells were treated with 80 and 200 μM of Resv, respectively, for 24 hours. We analyzed PL with radiolabeled inorganic phosphate (32Pi) by thin-layer chromatography, FA by gas chromatography-mass spectrometry, and lipid metabolism enzymes (DGAT2, FAS, ρACCβ, pAMPKα, and AMPK) by Western blot. Resv treated MDA-MB-231 phospholipids showed a reduction in phosphatidylcholine (63%) and phosphatidylethanolamine (35%). We observed an increase in eicosapentaenoic acid (EPA) (73%) and docosahexaenoic acid (DHA) (65%) in MCF-7 cells after Resv treatment. Interestingly, the same treatment caused 50% and 90% increases in EPA and DHA, respectively, in MDA-MB-231 cells. In MCF-7 cells, Resv increased the expression of ρACCβ (3.3-fold) and AMPKα/ρAMPKα (1.5-fold) and in MDA-MB-231 cells it inhibited the expression of ρACCβ (111.8-fold) and AMPKα/ρAMPKα (1.2 fold). Our results show that Resv modified PL and saturated and unsaturated FA especially in MDA-MB-231 cells, and open new perspectives to the understanding of the reported anticancer effect of Resv on these cells.

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Distinction between breast cancer cell subtypes using third harmonic generation microscopy

Cited by 28 publications

References 54 publications

Non-Linear Microscopy: A Well-Established Technique for Biological Applications towards Serving as a Diagnostic Tool for in situ Cultural Heritage Studies

Non-Linear Microscopy: A Well-Established Technique for Biological Applications towards Serving as a Diagnostic Tool for in situ Cultural Heritage Studies

Tensor regularized total variation for denoising of third harmonic generation images of brain tumors

Resveratrol Modifies Lipid Composition of Two Cancer Cell Lines

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