Distinction between dysplasia-associated lesion or mass (DALM) and adenoma in patients with ulcerative colitis

Fogt, Franz; Urbanski, S. J.; Sanders, ME; Furth, Emma E.; Zimmerman, R. L.; Deren, Julius J.; Noffsinger, Amy; Vortmeyer, Alexander O.; Hartmann, Christopher J.; Odze, Robert D.

doi:10.1016/s0046-8177(00)80240-3

Cited by 58 publications

(40 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[24][25][26][27] With the crypt isolation technique, one can avoid contamination from many stromal and inflammatory cells, providing an adequate amount of high-quality DNA. In addition, the use of automated high-resolution fluorescence-based quantification and size determination by GeneScan software could easily improve the band definition.…”

Section: Discussionmentioning

confidence: 99%

“…Because DNA isolated from nonfixed, immediately digested cells is less damaged than that isolated from fixed cells, we analyzed genetic changes of nondysplastic epithelium using a crypt isolation technique and analysis with GeneScan software (Perkin-Elmer Cetus, Norwalk, CT, USA). [24][25][26][27] This method can prevent contamination by many inflammatory cells, stromal cells, and degenerating cells, and may enable a better understanding of the earlier steps of UC carcinogenesis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microsatellite instability and loss of heterozygosity in the nondysplastic colonic epithelium of ulcerative colitis

Takahashi

Kojima

Kinouchi

et al. 2003

Journal of Gastroenterology

View full text Add to dashboard Cite

Background. A major longterm risk for patients with ulcerative colitis (UC) is the development of colorectal dysplasia and cancer. Microsatellite instability (MI) has now been reported not only in colitic cancers but also in dysplasias, and even in nondysplastic inflamed mucosa. With the conventional microdissection technique, however, contamination by nonepithelial cells cannot be prevented and this could produce less reliable results than other methods. Therefore, we examined the condition of MI and loss of heterozygosity (LOH) of UC epithelium using a crypt isolation technique. Methods. One hundred and twenty-nine biopsy samples from 21 patients with UC were investigated for histology and microsatellite status, using nine microsatellite markers. A total of 1031 polymenase chain reaction (PCR) products were evaluated. Results. We found that no microsatellite markers displayed instability, but LOH at the 3p locus was detected in the nondysplastic epithelium of one patient with longstanding UC. Conclusions. Our study strongly suggests that MI does not contribute to the progression of the colitis-dysplasia sequence.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microsatellite instability and loss of heterozygosity in the nondysplastic colonic epithelium of ulcerative colitis

Takahashi

Kojima

Kinouchi

et al. 2003

Journal of Gastroenterology

View full text Add to dashboard Cite

show abstract

“…The 'flatness' of the former adenomas is attributed to the genetic background of the patients concerned. Similarly, in ulcerative colitis (UC)-associated patients, UC-adenomas are reported to be genetically and biologically similar to sporadic adenomas, suggesting that UC associated carcinomas initiate via adenoma-like dysplasia-associated lesions [134].…”

Section: Linking the Main Tumorigenesis Pathwaysmentioning

confidence: 99%

Recent advances in colorectal cancer genetics and diagnostics

Cheah

2009

Critical Reviews in Oncology/Hematology

View full text Add to dashboard Cite

“…31 The term dysplasia-associated lesion or mass (DALM) has been adopted to describe the group of ''endoscopically visible'' lesions, but in fact refers to a heterogeneous population of lesions which demonstrate plaque-like, mass, stricture, sessile or pedunculated morphology. 32 Indeed, sessile or pedunculated DALMs with conventional endoscopy can resemble sporadic adenomas. 32 33 Accurate diagnosis of such lesions is of importance, as an adenoma-like DALM is highly associated with the presence of invasive CRC.…”

Section: Current Evidence Base For Colorectal Cancer Screening In Chrmentioning

confidence: 99%

Techniques for targeting screening in ulcerative colitis

Hurlstone

Brown

2007

Postgraduate Medical Journal

View full text Add to dashboard Cite

Patients with longstanding chronic ulcerative colitis are “at risk” of developing colorectal cancer. Approximately 1 in 6 patients will die as a result of colorectal malignancy, which can often be difficult to detect using conventional “white light” colonoscopy. New endoscopic techniques and technologies including the use of dye sprays, “chromoendoscopy”, high magnification chromoscopic colonoscopy and recently chromoscopic assisted confocal laser scanning in vivo endomicroscopy have now been introduced to improve the diagnostic yield of intraepithelial neoplasia at screening colonoscopy. This review details the true “risk” of colorectal cancer complicating ulcerative colitis, discusses the objective evidence to support current endoscopic screening guidelines, and describes the imminent technological paradigm shift about to occur in the endoscopic management and detection of intraepithelial neoplasia.

show abstract

Distinction between dysplasia-associated lesion or mass (DALM) and adenoma in patients with ulcerative colitis

Cited by 58 publications

References 7 publications

Microsatellite instability and loss of heterozygosity in the nondysplastic colonic epithelium of ulcerative colitis

Microsatellite instability and loss of heterozygosity in the nondysplastic colonic epithelium of ulcerative colitis

Recent advances in colorectal cancer genetics and diagnostics

Techniques for targeting screening in ulcerative colitis

Contact Info

Product

Resources

About