Distinctive Clinical and Pathologic Features of Immature Teratomas Arising from Induced Pluripotent Stem Cell-Derived Beta Cell Injection in a Diabetes Patient

Han, Lei; Hao, He; Yang, Yihao; Meng, Qingyin; Ye, Fan; Chen, Gong; Zhang, Jing

doi:10.1089/scd.2021.0255

Cited by 30 publications

(14 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another advantage for the approach detailed in our study is that the PSCs were differentiated in vivo, thus mitigating any potential future risk for residual PSCs to form teratomas in patients as recently reported for in vitro PSC-derived cells (65). Last, as iPSCs differentiate into satellite cells in postnatal chimeras, this approach ensures the generation of adult muscle stem cells, in comparison to myogenic cells generated from PSCs via directed differentiation in vitro, which may carry embryonic attributes (66).…”

Section: Discussionmentioning

confidence: 82%

Interspecies generation of functional muscle stem cells

Domenig

Lenardič

Zvick

et al. 2023

Preprint

View full text Add to dashboard Cite

Satellite cells, the stem cells of skeletal muscle tissue, hold a prodigious regeneration capacity. However, low satellite cell yield from autologous or donor-derived muscles precludes adoption of satellite cell transplantation for the treatment of muscle diseases including Duchenne muscular dystrophy (DMD). To address this limitation, here we investigated whether sufficient quantity of satellite cells can be produced in allogeneic or xenogeneic animal hosts. First, we report on exclusive satellite cell production in intraspecies mouse chimeras by injection of CRISPR/Cas9-corrected DMD-induced pluripotent stem cells (iPSCs) into blastocysts carrying an ablation system of host Pax7+ satellite cells. Additionally, injection of genetically-corrected DMD-iPSCs into rat blastocysts produced interspecies rat-mouse chimeras harboring mouse muscle stem cells that efficiently restored dystrophin expression in DMD mice. This study thus provides a proof-of-principle for the generation of therapeutically-competent stem cells between divergent species, raising the possibility of procuring human stem cells in large animals for regenerative medicine purposes.

show abstract

Section: Discussionmentioning

confidence: 82%

Interspecies generation of functional muscle stem cells

Domenig

Lenardič

Zvick

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Improving the safety of stem-cell-derived beta cell transplantation with an inducible safety switch Simon N Chu 1,4,5 , N Shabrina Amirruddin 2,3,4,5 , Devesh K Sharma 1,5 , Isabel C Sierra 2,3,4,5 , Peter G Stock 1 , Julie B Sneddon 2,3,4,5 , M Kyle Cromer 1,5,6 .…”

Section: 8mentioning

confidence: 99%

410.8: Improving the safety of stem-cell-derived beta cell transplantation with an inducible safety switch

Chu,

Amirruddin,

Sharma

et al. 2023

Transplantation

View full text Add to dashboard Cite

“… 16 However, SC-islets also demonstrated potential for tumorigenicity in the form of teratoma and other malignant tumors. 17 , 18 Therefore, from a safety perspective, the currently favored intrahepatic approach may not be a safe way to transplant SC-islets since they are not retrievable. A major objective in moving stem cell-derived β cell technology to clinical application is to identify an optimal site for implantation.…”

Section: Introductionmentioning

confidence: 99%