Distinctive expression profiles of Caveolin-1 and Notch-1 protein in patients with nasal polyps or sinonasal inverted papillomas

Karagianni, Fani; Kataki, Agapi; Koniaris, Eythymios; Karamagkiolas, Sotirios; Derventzi, Anastasia; Giotakis, Evangelos; Konstandoulakis, Manousos M.; Zografos, George; Giotakis, Ioannis

doi:10.1016/j.prp.2018.09.029

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Meanwhile, Cao et al demonstrated that the increased level of IL‐17 + cells is associated with neutrophil infiltration in NIP tissues 14 . On the other hand, Yuan et al and Karagianni et al described dysregulated epithelial cell morphology in NIP, including ciliated and goblet cell differentiation were inhibited, basal cell hyperplasia, squamous metaplasia, and hyalinization of the basement membrane 15,16 . It implied that abnormal proliferation and differentiation were performed in the nasal epithelium of NIP.…”

Section: Introductionmentioning

confidence: 99%

Epithelial Tight Junction Anomalies in Nasal Inverted Papilloma

et al. 2023

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ObjectivesAs a critical component of the epithelial barrier, tight junctions (TJs) are essential in nasal mucosa against pathogen invasion. However, the function of TJs has rarely been reported in nasal inverted papilloma (NIP). This study aims to investigate the potential factors of TJs' abnormality in NIP.MethodsWe assessed the expression of ZO‐1, occludin, claudin‐1, claudin‐3, and claudin‐7 in healthy controls and NIP by real‐time quantitative polymerase chain reaction and immunofluorescent staining. The correlation between TJs expression and neutrophil count, TH1/TH2/TH17 and regulatory T cell biomarkers, and the proportion of nasal epithelial cells was investigated.ResultsUpregulation of ZO‐1, occludin, claudin‐1, and claudin‐7, along with downregulation of claudin‐3, was found in NIP compared to control (all p < 0.05). An abnormal proportion with a lower number of ciliated cells (control vs. NIP: 37.60 vs. 8.67) and goblet cells (12.52 vs. 0.33) together with a higher number of basal cells (45.58 vs. 124.00) in NIP. Meanwhile, claudin‐3 was positively correlated with ciliated and goblet cells (all p < 0.01). Additionally, neutrophils were excessively infiltrated in NIP, negatively correlated with ZO‐1, but positively with claudin‐3 (all p < 0.05). Furthermore, FOXP3, IL‐10, TGF‐β1, IL‐5, IL‐13, and IL‐22 levels were induced in NIP (all p < 0.01). Occludin level was negatively correlated with IL‐10, IL‐5, IL‐13, and IL‐22, whereas ZO‐1 was positively with TGF‐β1 (all p < 0.05).ConclusionNasal epithelial barrier dysfunction with TJs anomalies is commonly associated with abnormal proliferation and differentiation of epithelial cells and imbalance of immune and inflammatory patterns in NIP.Level of EvidenceNA Laryngoscope, 2023

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Section: Introductionmentioning

confidence: 99%

Epithelial Tight Junction Anomalies in Nasal Inverted Papilloma

et al. 2023

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“…In a mouse model of chronic allergic rhinitis, treatment with DAPT (N‐[N‐(3,5‐Difluorophenacetyl)‐L‐alanyl]‐S‐phenylglycine t‐butyl ester), a γ‐secretase inhibitor, alleviated upper airway inflammation by suppressing Th2 cytokine levels, reducing eosinophils infiltration and goblet cells metaplasia (Shi et al, 2017 ). Furthermore, differential expression of NOTCH1 has been observed between NPs and sinonasal inverted papillomas (Karagianni et al, 2018 ). Notch regulates the expression of endothelial nitric oxide synthase (eNOS) (Patenaude et al, 2014 ), clara (Club) cell 10 kDa protein (CC10) (Motooka et al, 2017 ), and transforming growth factor‐β (TGF‐β) (Kluppel & Wrana, 2005 ), all known to be differently regulated in NPs compared to normal mucosa (Coste et al, 1998 ; Fritz et al, 2003 ; Koennecke et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Notch pathway in nasal polyps of patients with chronic rhinosinusitis: A pilot study

Aquila

Alaimo

Marracino

et al. 2022

Physiological Reports

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Chronic rhinosinusitis with nasal polyps is a widespread pathology characterized by persistent inflammation of nasal and paranasal mucosa. Although it represents one of the most frequent diseases of the nasal cavities, its etiology is still not completely elucidated. There is evidence suggesting that the Notch signaling, a highly conserved intercellular pathway known to regulate many cellular processes, including inflammation, is implicated in nasal polyps formation. The purpose of this study was to investigate the expression of genes of the Notch pathway in nasal polyps from patients with chronic rhinosinusitis. Nasal polyps and adjacent mucosa tissue were obtained from 10 patients. RNA was analyzed by quantitative reverse transcriptase‐polymerase chain reaction for the expression level of (1) Notch pathway components such as receptors (NOTCH1‐4), ligands (DLL4, JAGGED‐1), and target genes (HEY1, 2, and HES1) and (2) genes providing information on the pathogenesis of polyposis (C‐MYC and SCGB1A1) and on eosinophils content (CCL26, IL5, and SAA2). We report a Notch‐driven gene expression pattern in nasal polyps which correlates with the expression of genes highly expressed in eosinophils, whose presence is an important parameter to define the pathophysiologic diversity characterizing nasal polyps. Taken together, our results suggest a role for Notch signaling in the pathophysiology of polyposis. Further studies are needed to elucidate the role of Notch in nasal polyps formation and to establish whether it could represent a novel therapeutic target for this pathology.

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Multimodal management of PD‐L1 positive sinonasal inverted papilloma with malignant transformation

Velazquez,

Hakim,

Brockhoff

et al. 2024

World j. otorhinolaryngol.-head neck surg.

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Inverted papillomas (IP) are rare sinonasal tumors (Schneiderian papillomas) characterized by local invasion, with high recurrence rates and potential for malignant transformation into squamous cell carcinoma (SCC ex‐IP). Traditionally, the mainstay of treatment has been surgical resection, often challenging due to invasion of the skull base or vital neurovascular structures. In advanced cases, prognosis is poorer and radiation therapy is the current standard of care with limited role of systemic therapy. We present a case of unresectable SCC ex‐IP treated with sequential chemoradiation and PD‐1 immunotherapy (pembrolizumab) and achieving complete response at 1 year. This case suggests that immunotherapy can effectively treat SCC ex‐IP and we propose that programmed death ligand‐1 (PD‐L1) testing should be performed in all advanced cases. Prospective studies are needed to validate immunotherapy in adjuvant or frontline treatments for advanced SCC ex‐IP, and the role of PD‐L1 as a predictive biomarker.

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Distinctive expression profiles of Caveolin-1 and Notch-1 protein in patients with nasal polyps or sinonasal inverted papillomas

Cited by 3 publications

References 29 publications

Epithelial Tight Junction Anomalies in Nasal Inverted Papilloma

Epithelial Tight Junction Anomalies in Nasal Inverted Papilloma

Characterization of the Notch pathway in nasal polyps of patients with chronic rhinosinusitis: A pilot study

Multimodal management of PD‐L1 positive sinonasal inverted papilloma with malignant transformation

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