Distinguishing between archaeological sheep and goat bones using a single collagen peptide

Buckley, Michael; Kansa, Sarah Whitcher; Howard, Sarah; Campbell, Stuart; Thomas‐Oates, Jane; Collins, Matthew J.

doi:10.1016/j.jas.2009.08.020

Cited by 282 publications

(218 citation statements)

References 25 publications

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“…Finally, one bone specimen was identified as Capra sp. on the basis of diagnostic peptide marker G (3093.4; Buckley et al, 2010;Campana et al, 2013) in combination with 1180.2 for marker A.…”

Section: Zooms Sample Resultsmentioning

confidence: 99%

“…), different species in the genus Equus (identified as Equidae), differentiation in the genus Capra (identified as Capra sp. ; Buckley et al, 2010;Campana et al, 2013) and separation between Elephantidae . Identifications as "Cervid/Saiga" cannot be improved proteomically, despite marker A being absent in three out of four of such identifications (marker A would be 1180.6 þ 1196.6 for both Saiga tatarica and relevant Cervid species).…”

Section: Taxa Identificationmentioning

confidence: 99%

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Using ZooMS to identify fragmentary bone from the Late Middle/Early Upper Palaeolithic sequence of Les Cottés, France

Welker

Soressi

Rendu

et al. 2015

Journal of Archaeological Science

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103

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a b s t r a c tWe report the application of a molecular barcode method (ZooMS) to identify fragmentary bone remains (>2.5 cm) from a Middle to Upper Palaeolithic sequence at Les Cott es, France. ZooMS uses peptide mass fingerprinting of collagen (the most abundant protein in bone) to discriminate fauna (typically to genus level). Using previously reported peptide markers we initially conducted a blind test using 34 morphologically identified bones, followed by the application of ZooMS on 145 morphologically unidentified bone specimens. For the blind test, ZooMS was in agreement with morphological identifications in all cases, but in some instances taxonomic resolution is lower than morphological identifications. Further, 93.8% (136/145) of spectra obtained for morphologically unidentified bone specimens result in identifications that cannot be taxonomically improved by ZooMS. These include ten bone specimens showing signs of carnivore digestion. Focussing on the unidentified bone specimens of the Châtelper-ronian unit at Les Cott es (US06), ZooMS identified an additional z30% of the total number of bones discovered, increasing the total number of identified bone specimens to 61.8%. Further, ZooMS revealed higher taxonomic richness compared to morphological identifications for US06, thereby providing a more informed interpretation of the faunal community present at Les Cott es during the Châtelperronian.

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Section: Zooms Sample Resultsmentioning

confidence: 99%

Section: Taxa Identificationmentioning

confidence: 99%

Using ZooMS to identify fragmentary bone from the Late Middle/Early Upper Palaeolithic sequence of Les Cottés, France

Welker

Soressi

Rendu

et al. 2015

Journal of Archaeological Science

Self Cite

103

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“…Samples were analyzed using a calibrated Ultraflex III (NLD1; Bruker Daltonics) MALDI-TOF instrument in reflector mode. Spectral analysis was performed using the open-source cross-platform software mMass (www.mmass.org) (42), and individual peptides were identified manually according to Buckley et al (43,44 …”

Section: Methodsmentioning

confidence: 99%

Animal origin of 13th-century uterine vellum revealed using noninvasive peptide fingerprinting

Fiddyment

Holsinger

Ruzzier

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

158

134

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Tissue-thin parchment made it possible to produce the first pocket Bibles: Thousands were made in the 13th century. The source of this parchment, often called "uterine vellum," has been a long-standing controversy in codicology. Use of the Latin term abortivum in many sources has led some scholars to suggest that the skin of fetal calves or sheep was used. Others have argued that it would not be possible to sustain herds if so many pocket Bibles were produced from fetal skins, arguing instead for unexpected alternatives, such as rabbit. Here, we report a simple and objective technique using standard conservation treatments to identify the animal origin of parchment. The noninvasive method is a variant on zooarchaeology by mass spectrometry (ZooMS) peptide mass fingerprinting but extracts protein from the parchment surface by using an electrostatic charge generated by gentle rubbing of a PVC eraser on the membrane surface. Using this method, we analyzed 72 pocket Bibles originating in France, England, and Italy and 293 additional parchment samples that bracket this period. We found no evidence for the use of unexpected animals; however, we did identify the use of more than one mammal species in a single manuscript, consistent with the local availability of hides. These results suggest that ultrafine vellum does not necessarily derive from the use of abortive or newborn animals with ultrathin hides, but could equally well reflect a production process that allowed the skins of maturing animals of several species to be rendered into vellum of equal quality and fineness. Hamlet, V.i

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“…It has been applied more recently to the analysis of proteins in ancient bones (102,103) and eggshells (104), where it allows the identification of species, and has been used to analyse proteinaceous and lipid binders in works of art and wall plasters (105)(106)(107)(108). It has been used to analyze proteinaceous glue from a wooden building (109), to study the photo-degradation of terpenoid varnishes (110), and to assess the condition and penetration of polyethylene glycol in preserved ships (111).…”

Section: New Mass Spectrometric Techniquesmentioning

confidence: 99%

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Steele

2013

ACS Symposium Series

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The analysis of organic residues from archaeological materials has become increasingly important to our understanding of ancient diet, trade and technology. Residues from diverse contexts have been retrieved and analysed from the remains of food, medicine and cosmetics to hafting material on stone arrowheads, pitch and tar from shipwrecks, and ancient manure from soils. Research has brought many advances in our understanding of archaeological, organic residues over the past two decades. Some have enabled very specific and detailed interpretations of materials preserved in the archaeological record. However there are still areas where we know very little, like the mechanisms at work during the formation and preservation of residues, and areas where each advance produces more questions rather than answers, as in the identification of degraded fats. This chapter will discuss some of the significant achievements in the field over the past decade and the ongoing challenges for research in this area.1341280_File000001_20903114.docPrinted 7/19/2013 2

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Distinguishing between archaeological sheep and goat bones using a single collagen peptide

Cited by 282 publications

References 25 publications

Using ZooMS to identify fragmentary bone from the Late Middle/Early Upper Palaeolithic sequence of Les Cottés, France

Using ZooMS to identify fragmentary bone from the Late Middle/Early Upper Palaeolithic sequence of Les Cottés, France

Animal origin of 13th-century uterine vellum revealed using noninvasive peptide fingerprinting

Organic Residues in Archaeology: The Highs and Lows of Recent Research

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