Distribution and in situ proliferation patterns of intravenously injected immortalized human neural stem-like cells in rats with focal cerebral ischemia

Noninvasive method of immortalized neural stem-like cell transplantation in an experimental model of Huntington's disease

“…In the case of genetically manipulated human NSC clones (v-myc+), however, the propagating gene product v-myc is undetectable in donor human cells after engraftment (Flax et al, 1998), despite the fact that the brains of transplant recipients contain numerous stably engrafted donor-derived cells. In addition, previous observations showed the invariant absence of brain tumors derived from implanted v-myc-propagated NSCs, even after several years in rodents (Snyder et al, 1992;Jeong et al, 2003;Chu et al, 2003Chu et al, , 2004a or in primates (Ourednik et al, 2001), which is consistent with the loss of v-myc expression from stably engrafted NSCs following transplantation. We also detected no tumorigenesis in the present study.…”

Human neural stem cells overexpressing glial cell line-derived neurotrophic factor in experimental cerebral hemorrhage

“…3,4 Neural stem cells (NSCs) have recently received a great deal of attention and interest for their potential in neurological disorders, [5][6][7][8][9] and several recent studies have indicated that NSCs engrafted in animal models of stroke survive and ameliorate neurological deficits in the animals. [10][11][12][13][14][15][16][17] We have shown earlier that intravenously or intracerebrally transplanted human NSCs (HB1.F3) migrated selectively to the damaged brain sites caused by ischemia [11][12][13] or ICH, [14][15][16][17][18] differentiated into neurons and astrocytes, and promoted functional recovery in these animals. However, low survival rate of grafted F3 NSCs in ischemia and ICH rats in the earlier studies is a serious concern; less than 50% of grafted NSCs survived in ICH mice at 2 weeks post-transplantation.…”

“…GDNF, a member of the transforming growth factor-b superfamily, is a potent neurotrophic factor that promotes the survival of central nervous system neurons by activating several survival signal pathways and inhibits death signals. [21][22][23] Considering the evidence of functional recovery in stroke animal models after brain transplantation of human NSCs [10][11][12][13][14][15][16][17][18] or GDNF treatment, [24][25][26][27][28][29] we wished to investigate in this study whether the human NSCs overexpressing GDNF, by pairing clonal human NSCs with GDNF gene, can lead to the increased cell survival and functional improvement in mouse ICH stroke model.…”

Transduction of Neural Precursor Cells with TAT-Heat Shock Protein 70 Chaperone: Therapeutic Potential Against Ischemic Stroke after Intrastriatal and Systemic Transplantation

“…Among different transplantation routes, intravenous cell delivery has been used due to its simplicity and clinical practicability [8,25,26], although achieving low intracerebral cell numbers [15,27]. Therefore, local intracerebral injections are widely used, which can enhance the number of transplanted intracerebral cells but lack clinical utility due to invasive delivery procedures [28][29][30][31][32].…”