Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq

ALYOUSIF, NASSIR ABDULLAH

doi:10.13057/biodiv/d230209

Cited by 9 publications

(8 citation statements)

References 32 publications

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“…As a spore-forming and Gram-positive organism, it can survive under many conditions in the environment and colonize a range of environments including saline soils (Song et al, 2017). The other organism identified as Cytobacillus species which is related to the Bacillus genus are commonly found in the soil as well (Wei et al, 2016;Alyousif, 2022). Previously known as Bacillus Firmus, several pieces of evidence have shown that this organism is distinct from members of the Bacillus genus and can survive in the soil for long periods, and has been reported from many types of soils (Patel and Gupta, 2020).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Local Soil Bacteria with Larvicidal Activities Against Aedes aegypti Larvae

Alotaibi¹,

Saidi²,

Roushdy³

et al. 2022

OnLine Journal of Biological Sciences

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Mosquito-borne diseases caused by infected mosquitoes like dengue fever, Zika fever, Chikungunya and malaria are spread to humans through biting causing disease epidemics all over the world. This indicates the necessity for new mosquito-borne disease control strategies in Saudi Arabia and internationally. This study evaluated the potential of local bacteria isolated from the soil of the Rahat region of Makkah, Saudi Arabia for the biocontrol of Aedes aegypti larvae, a major agent for the transmission of dengue fever. Soil samples were collected from the Rahat region, Makkah for the isolation of bacteria. The bacteria were evaluated for larvicidal activity against the larvae of A. aegypti. The bacteria that show toxicity to the larvae were identified using morphological and molecular characteristics. Four different strains of the bacteria with a toxicity towards A. aegypti larvae were isolated and identified. Two of the isolates (Brevibacillus centrosporus N8 and Cytobacillus species N7) caused 100% mortality in 24 h while the other two isolates (Escherichia coli N3 and Escherichia coli N4) caused at least 70% mortality. The findings of this study revealed the larvicidal activity of soil bacteria from the Rahat region, Makkah and could be a potential candidate for the biocontrol of mosquito vectors.

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Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Local Soil Bacteria with Larvicidal Activities Against Aedes aegypti Larvae

Alotaibi¹,

Saidi²,

Roushdy³

et al. 2022

OnLine Journal of Biological Sciences

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“…Microbial diversity particularly in genetic diversity still is an undergoing aspect of current research from bacteria, archaea fungi, and the microbial origin from the rhizosphere, phyllosphere, soil, and plant pathogens. For example, the total Bacillus related bacterial species were investigated from different soil environments (Alyousif 2022). Among them, the BOX fingerprint technique was applied to soybean phyllosphere bacteria diversity (Nurcahyanti et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Short Communication: Genetic diversity of walnut blight-associated bacteria from China using rep-PCR

et al. 2022

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Abstract. Zheng C, Zhu J, Yang X, Fu B. 2022. Short Communication: Genetic diversity of walnut blight-associated bacteria from China using rep-PCR. Biodiversitas 23: 5681-5686. Walnut blight is caused by the bacterium Xanthomonas arboricola pv. juglandis (Xaj), is one of the most serious diseases in walnut production around the world. Understanding the genetic diversity of walnut blight pathogen is of great significance to developing a scientific and reasonable prevention and control strategy. The rep-PCR (Repetitive Sequence Polymerase Chain Reaction) technique is a powerful tool for analyzing plant pathogens. In this paper, using three pairs of rep-PCR universal primers (ERIC, BOX, and REP), a genetic diversity analysis was carried out on walnut blight-associated bacteria mainly isolated from different counties of Hubei Province, China. A study of 60 walnut blight-associated bacteria isolated in 2016 found that they had a high genetic similarity among them. There is no clear correlation between the genetic diversity of these isolates and their geographical or tissue origins. Interestingly, these clusters can also distinguish copper-resistant isolates from sensitive ones. Therefore, rep-PCR is a powerful tool to study the population of walnut pathogenic bacteria.

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“…Bacillus is a big and diversified genus of rod-shaped, Gram-positive, endospore-forming bacteria that are aerobic and facultatively anaerobic, and they are a genus of the Bacillaceae family that belongs to the Firmicutes phylum (Errington and van der Aa 2020). In fact, it appears that the physiological aspects of Bacillus genus members have enabled them to inhabit almost all natural environment, such as water, air, soil, lake sediments, and fodder, and also harsh environments including thermal acid water, salt marshes, and hot springs (Gopal et al 2015;Alyousif 2022). The survival of Bacillus genus members for a long time in various extreme conditions is due to the formation of protective endospores that withstand unfavorable environmental conditions (Zammuto et al 2020).…”

Section: Bacterial Identification By 16s Rdnamentioning

confidence: 99%

Screening for extracellular synthesis of silver nanoparticles by bacteria isolated from Al-Halfaya oil field reservoirs in Missan province, Iraq

2022

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Abstract. Alshami HGA, Al-Tamimi WH, Hateet RR. 2022. Screening for extracellular synthesis of silver nanoparticles by bacteria isolated from Al-Halfaya oil field reservoirs in Missan province, Iraq. Biodiversitas 23: 3462-3470. Microorganisms that live in deep environments are thought to be adapted to the conditions of these environments and possess enzymatic systems that can be useful in scientific and commercial applications. The study aimed to isolate, identify, and screening of silver nanoparticles (AgNPs)-producing bacteria from five samples of produced water of the Al-Halfaya oil field reservoirs in Missan governorate, Iraq. The results revealed a total of 22 bacterial isolates were isolated and identified by morphological features and amplification of the 16S rDNA gene. The isolates are belonging to nine species, including Bacillus cereus strain DBA1.1, Bacillus thuringiensis strain MSP51, Neobacillus drentensis strain ROA042, Enterococcus faecalis strain 2674, Exiguobacterium mexicanum strain AB201, Klebsiella quasipneumoniae strain KP18-31, Klebsiella quasipneumoniae subsp. similipneumoniae strain 2437, Klebsiella pneumoniae strain IOB-L, and Acinetobacter lwoffii strain K34. The Gram-positive bacteria were the most dominant (55.56%), and the most frequent isolate was Klebsiella quasipneumoniae strain KP18-31 (63.64%). Six species out of nine species were recognized as AgNPs producing bacteria and B. cereus strain DBA1 was the best and most potent isolate in the synthesis of AgNPs. The phylogenetic tree was constructed depending on 16S rDNA gene sequences to determine the evolutionary relationship among the isolated bacteria.

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Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq

Cited by 9 publications

References 32 publications

Isolation and Identification of Local Soil Bacteria with Larvicidal Activities Against Aedes aegypti Larvae

Isolation and Identification of Local Soil Bacteria with Larvicidal Activities Against Aedes aegypti Larvae

Short Communication: Genetic diversity of walnut blight-associated bacteria from China using rep-PCR

Screening for extracellular synthesis of silver nanoparticles by bacteria isolated from Al-Halfaya oil field reservoirs in Missan province, Iraq

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