Distribution of PDIA3 transcript and protein in rat testis and sperm cells

XJ, Zhao; Tang, RZ; Ml, Wang; Guo, WL; Liu, J; Li, L; Xing, WJ

doi:10.1111/j.1439-0531.2012.02024.x

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…The mice injected with these proteins in CFA had induced EAO at a rate of 25%. PH-20, disulphide isomerase endoplasmic reticulum 60, heat-shock 70 kDa protein 5, and sperm outer dense fiber major protein 2 were expressed in the testis and epididymis in humans and mice 40 41 42 43 . Heterogeneous nuclear ribonucleoprotein H1 was expressed at testicular somatic cells, including Sertoli and Leydig cells, at 0 and 1 week old in mice 36 .…”

Section: Discussionmentioning

confidence: 98%

Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone

Terayama

Hirai

Naito

et al. 2016

Sci Rep

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There are various autoimmunogenic antigens (AIs) in testicular germ cells (TGCs) recognized as foreign by the body’s immune system. However, there is little information of TGC-specific AIs being available. The aim of this study is to identify TGC-specific AIs. We have previously established that immunization using viable syngeneic TGC can also induce murine experimental autoimmune orchitis (EAO) without using any adjuvant. This study is to identify TGC-specific AIs by TGC liquid chromatography–tandem mass spectrometry analysis, followed by two-dimensional gel electrophoresis that reacted with serum IgG from EAO mice. In this study, we identified 11 TGC-specific AIs that reacted with serum from EAO mice. Real-time RT-PCR analysis showed that the mRNA expressions of seven TGC-specific AIs were significantly higher in only mature testis compared to other organs. Moreover, the recombinant proteins of identified 10 (except unnamed protein) TGC-specific AIs were created by using human embryonic kidney 293 (HEK293) cells and these antigencities were reconfirmed by Western blot using EAO serum reaction. These results indicated Atp6v1a, Hsc70t, Fbp1 and Dazap1 were candidates for TGC-specific AIs. Identification of these AIs will facilitate new approaches for understanding infertility and cancer pathogenesis and may provide a basis for the development of novel therapies.

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Section: Discussionmentioning

confidence: 98%

Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone

Terayama

Hirai

Naito

et al. 2016

Sci Rep

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“…Therefore, sperm development process should be error free. Biological process of high abundant proteins in HF were enriched with the Protein disulphide isomerase like PDIA1 and PDIA3 that regulate protein quality and redox regulation ( Ellerman et al, 2006 ; Zhao et al, 2013 ; Wong et al, 2017 ; Chichiarelli et al, 2022 ) Sp17 regulates acrosomal reaction and interactions with the zona pellucida during the fertilization process ( Chiriva-Internati et al, 2009 ). Motility is a unique property of spermatozoa because of which, it travels through the FRT to fertilise an egg.…”

Section: Discussionmentioning

confidence: 99%

Identification of protein candidates in spermatozoa of water buffalo (Bubalus bubalis) bulls helps in predicting their fertility status

Karanwal

Pal²,

Chera³

et al. 2023

Front. Cell Dev. Biol.

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The water buffalo (Bubalus bubalis) is an indispensable part of the Indian dairy sector and in several instances, the farmers incur economic losses due to failed pregnancy after artificial insemination (AI). One of the key factors for the failure of conception is the use of semen from the bulls of low fertilizing potential and hence, it becomes important to predict the fertility status before performing AI. In this study, the global proteomic profile of high fertile (HF) and low fertile (LF) buffalo bull spermatozoa was established using a high-throughput LC-MS/MS technique. A total of 1,385 proteins (≥1 high-quality PSM/s, ≥1 unique peptides, p < 0.05, FDR < 0.01) were identified out of which, 1,002 were common between both the HF and LF groups while 288 and 95 proteins were unique to HF and LF groups respectively. We observed 211 and 342 proteins were significantly high (log Fc ≥ 2) and low abundant (log Fc ≤ 0.5) in HF spermatozoa (p < 0.05). Gene ontology analysis revealed that the fertility associated high abundant proteins in HF were involved in spermatogenesis, sperm motility, acrosome integrity, zona pellucida binding and other associated sperm functions. Besides this, the low abundant proteins in HF were involved in glycolysis, fatty acid degradation and inflammation. Furthermore, fertility related differentially abundant proteins (DAPs) on sperm viz., AKAP3, Sp17, and DLD were validated through Western blotting and immunocytochemistry which was in coherence with the LC-MS/MS data. The DAPs identified in this study may be used as potential protein candidates for predicting fertility in buffaloes. Our findings provide an opportunity in mitigating the economic losses that farmers incur due to male infertility.

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“…PDIA3 has recently been found in human and mouse sperm cells. It may affect the sperm–egg fusion [ 34 ]. Although PDIA3 was initially described as a resident protein of the endoplasmic reticulum (ER), it was also shown on the cell surface [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of the Season and Region Factor on Phosphoproteome of Stallion Epididymal Sperm

Dyrda

Orzołek

Ner‐Kluza

et al. 2021

Animals

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Epididymal maturation can be defined as a scope of changes occurring during epididymal transit that prepare spermatozoa to undergo capacitation. One of the most common post-translational modifications involved in the sperm maturation process and their ability to fertilise an oocyte is the phosphorylation of sperm proteins. The aim of this study was to compare tyrosine, serine, and threonine phosphorylation patterns of sperm proteins isolated from three subsequent segments of the stallion epididymis, during and out of the breeding season. Intensities of phosphorylation signals and phosphoproteins profiles varied in consecutive regions of the epididymis. However, significant differences in the phosphorylation status were demonstrated in case of endoplasmic reticulum chaperone BiP (75 and 32 kDa), protein disulfide-isomerase A3 (50 kDa), nesprin-1 (23 kDa), peroxiredoxin-5 (17 kDa), and protein bicaudal D homolog (15 kDa) for season x type of phosphorylated residues variables. Significant differences in the phosphorylation status were also demonstrated in case of endoplasmic reticulum chaperone BiP and albumin (61 kDa), protein disulfide-isomerase A3 (50 kDa), and protein bicaudal D homolog (15 kDa) for region x type of phosphorylated residues variables.

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Distribution of PDIA3 transcript and protein in rat testis and sperm cells

Cited by 8 publications

References 29 publications

Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone

Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone

Identification of protein candidates in spermatozoa of water buffalo (Bubalus bubalis) bulls helps in predicting their fertility status

Influence of the Season and Region Factor on Phosphoproteome of Stallion Epididymal Sperm

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