Distribution of Progesterone to the Uterus and Associated Vasculature of Cattle.

Abstract

Multiparous dairy cows were sampled to study the concentrations of progesterone in tissue of the uterus and associated vasculature and to determine whether progesterone was delivered to the uterus locally. In study 1, progesterone was greater (p<0.05) in the first venous branch draining the cranial portion of the uterine cornu adjacent to the ovary with a corpus luteum than in jugular blood or in the same vein draining the opposite uterine cornu on day 11 postestrus.Concentrations of progesterone were also greater (p<0.05) in the cranial than in the caudal half of the uterine cornu adjacent to the luteal-bearing ovary or in the cranial and caudal halves of the opposite uterine cornu. Concentrations of progesterone were also greater (p<0.05) in the uterine or ovarian arterial tissue adjacent to the ovary with the corpus luteum than in those same vessels on the contralateral side. In a second study, progesterone at 0 h on day 11 postestrus was greater (p 0.05) in the first venous branch draining the cranial portion of the uterine horn adjacent to the luteal-bearing ovary than in jugular blood, the same vein in the contralateral uterine cornu or in the same uterine vein 48 h after ligation and resection of the oviductal vein adjacent to the ovary with the corpus luteum. It is concluded that progresterone is delivered locally to the uterus and associated vasculature and the route of local delivery appears to be via the oviductal vein.

Effect of progesterone on embryo survival

“…However, concentrations were 6-to 13-fold higher in uterine fluid on day 15 compared to all the other days and concentrations in the uterine horn ipsilateral to the corpus luteum were over 2-fold higher than those in the contralateral side. The concentration of progesterone is known to be higher in the tissue (Pope et al, 1982) and uterine horn ipsilateral to the corpus luteum in cattle while Weems et al (1988) have shown that the concentration of progesterone in the ipsilateral uterine vein is almost 2-fold higher than that in the contralateral vein, which was in turn similar to the concentration in jugular venous blood. Based on these data, it is tempting to conclude that the concentration of uterine RBP is sensitive to the concentrations of systemic progesterone.…”

Distribuição espacial de transcritos no trato reprodutivo e sua relação com o ambiente endócrino periovulatório no início do diestro de bovinos de corte

“…Specifically, abundance of PGR and OXTR was downregulated in the ipsilateral horn. Downregulation was possibly in response to the reported greater concentration of P4 in the uterine vein, uterine artery and ovarian artery adjacent to the ovary bearing the CL (WEEMS et al, 1988). Regarding the effect of region of the ipsilateral horn, the expression of PGR, ESR2, LPL, SERPINA14 and SLC2A1 was upregulated on the anterior region compared to the posterior.…”

“…Indeed, P4 concentration on tissues (mesosalpinx, uterine artery, ovarian artery, anterior and posterior region of uterine horn) in the ipsilateral horn was greater than in the opposite side in beef and dairy cows and in ewes MAURER;STORMSCHAK, 1982;WEEMS et al, 1988WEEMS et al, , 1989. Also the uterine and ovarian artery adjacent to the ovary bearing the CL had higher P4 concentration (WEEMS et al, 1988(WEEMS et al, , 1989.…”

“…Vários estudos mostram que há diferença nos cornos uterinos durante o ciclo estral, provavelmente regulados pelas diferenças hormonais e sua relação com o lado do CL WEEMS et al, 1988; (GINTHER, 1974;GINTHER, 1976), sendo que no lado ipsolateral ao CL as anastomoses entre ramificações da artéria uterina e ovariana são significativamente mais proeminentes (LAMOND; DROST, 1974;GINTHER, 1976). O ramo uterino da artéria ovariana é significativamente maior em diâmetro no lado ipsolateral ao ovário contendo o CL (média de 1.5mm) do que no lado oposto (média de 1.1mm).…”

“…As concentrações de P4 plasmática também foram mais altas na primeira ramificação venosa da região anterior do corno uterino ipsolateral ao CL do que na última ramificação da região posterior do mesmo corno, no corno contralateral e ainda da jugular. Também foi realizada a ligadura e secção da veia tubária nas ovelhas e resultando nos mesmos encontrados em vacas de leite, e induzindo a acreditar que há transferência de P4 pela veia tubária em ruminantes (WEEMS et al, 1988(WEEMS et al, , 1989 Transferência por contracorrente no pedículo vascular do ovário eleva a concentração de hormônios esteroides no sangue durante o ciclo estral de porcas (STEFAFICZYK-KRZYMOWSKA et al, 1994 (LIN et al, 2012;YANG et al, 2013), jacarés , entre outros, principalmente ao longo do ciclo estral mas não relacionados a protocolos hormonais desenvolvidos para aumentar a fertilidade.…”