Diurnal changes in C  N metabolism and response of rice seedlings to UV-B radiation

Yun, Hye-Jin; Lim, Sunhyung; Kim, Yang Min; Lee, Yejin; Lee, Seulbi; Lee, Deog-Bae; Park, Kee Woong; Sung, Jwakyung

doi:10.1016/j.jplph.2018.02.007

Cited by 10 publications

(9 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate developmental changes in the transcriptome and metabolites in the blades, we harvested the blades of the fifth leaves, as follows: sink (Day 1, emergence (<0.5 cm) from the fourth leaf ligule), sink-to-source (Day 3) and source (Day 5) ( Figure 1 ). The emergence of a new leaf usually takes place every five to six days [ 11 ], and an average time required for sink-to-source transition is 2.4 d [ 5 ]. From the RNA-seq analysis, we detected 26,750, 28,963 and 30,274 expressed genes from the sink, sink-to-source and source stages, respectively.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional Changes of Cell Wall Organization Genes and Soluble Carbohydrate Alteration during Leaf Blade Development of Rice Seedlings

Joo

Kim

Sung

2021

Plants

Self Cite

View full text Add to dashboard Cite

Plant cell walls have two constituent parts with different components and developmental stages. Much of the mystery concerning the mechanisms of synthesis, decomposition, modification, and so forth, has been resolved using omics and microscopic techniques. However, it still remains to be determined how cell wall development progresses over time after leaf emergence. Our focus in the present study was to expand our knowledge of the molecular mechanisms associated with cell wall synthesis in rice leaf blade during three distinct stages (sink, sink-to-source transition, and source). The RNA-seq, quantitative reverse transcription PCR (qRT-PCR) and carbohydrate concentrations were evaluated using developing fifth leaf blades harvested at different time points. The results revealed that some of the essential genes for the primary cell wall (PCW) were highly upregulated in the sink-to-source transition compared to the sink stage, whereas those essential to the secondary cell wall (SCW) displayed relatively higher levels (p < 0.05) during the source stage. The concentrations of soluble carbohydrates differed via type rather than stage; we observed higher monosaccharides during the sink stage and higher di- and oligo-saccharides during the sink-to-source transition and source stages. In conclusion, our findings suggest that the transcriptional regulation of plant cell wall biosynthesis genes are both synchronistic with and independent of, and directly and indirectly governed by, the abundance of soluble carbohydrates in the developing leaf blade, and, finally, raffinose is likely to play a transport role comparable to sucrose.

show abstract

Section: Resultsmentioning

confidence: 99%

Transcriptional Changes of Cell Wall Organization Genes and Soluble Carbohydrate Alteration during Leaf Blade Development of Rice Seedlings

Joo

Kim

Sung

2021

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…UV-B fluorescent lamps were erected at a certain height above the rice seedlings for constant exposure to UV light. Rice seedlings (about 35 days old) were subjected to UV-B radiation each day for 6 h between 10:00 a.m. and 4:00 p.m. continuously for 5 days following a previous study by others [ 23 ]. For non-stress conditions, we grow Nβ and Sβ plants under normal light conditions.…”

Section: Methodsmentioning

confidence: 99%

β-Sitosterol differentially regulates key metabolites for growth improvement and stress tolerance in rice plants during prolonged UV-B stress

Shahzad

Ewas

Harlina

et al. 2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background Elevated ultraviolet-B (UV-B) radiation is potentially deleterious to many organisms specifically crop plants and has become a global challenge. Rice is an exceptionally important staple food which is grown worldwide, and many efforts have been done recently to improve rice varieties against UV-B stress. This current study aims to investigate the effects of exogenous application of β-sitosterol (βSito) on growth improvement and tolerance level of rice plants against prolonged UV-B stress. The physiological and metabolic responses were evaluated in rice plants not supplemented with βSito (Nβ) and those supplemented with βSito (Sβ). Results The Nβ and Sβ plants were grown under non-stress (ns) and under prolonged UV-B stress (uvs) conditions and termed as Nβns, Sβns and Nβuvs, Sβuvs, respectively. The application of βSito contributes positively under non-stress and specifically to UV-B stress in terms of improving numerous physiological parameters associated with growth and development such as shoot and root length, RWC, whole plant biomass, chlorophyll pigments, and photosynthetic-related parameters (Pn, Gs, Tr, WUEi, Fv/Fm, and NPQ) in Sβ compared with Nβ plants. Moreover, enhanced oxidative stress tolerance of Sβuvs vs. Nβuvs plants under stress was attributed to low levels of ROS and substantial trigger in activities of antioxidant enzymes (SOD, POD, CAT, and APX). Metabolic analysis was performed using GC-TOFMS, which revealed higher accumulation of several key metabolites including organic acids, sugars, amino acids, and others in Sβuvs vs. Nβuvs plants, which were mainly reduced in Nβ plants under stress vs. non-stress conditions. Conclusion These results provide useful data regarding the important role of βSito on growth maintenance and modulation of several metabolites associated with osmotic and redox adjustments during UV-B stress tolerance in rice plants. Importantly, βSito-regulated plasticity could further be explored specifically in relation to different environmental stresses in other economically useful crop plants.

show abstract

“…In response to long-term exposure to gamma irradiation, more fractions of protein, lipid, amino acid, and polysaccharides were detected in black gram seeds than control, which correlated with increasing biochemical metabolites [12]. Sucrose content sharply declined in rice leaves exposed to short-term UV-B [6]; however, soluble sugar continuously accumulated in wheat leaves irradiated with gamma rays during the first 4-week treatment [5]. Although, UV-B irradiation resulted in generation of free radicals, which induced conversion of amino acids such as gamma-aminobutyric acid and glutathione into antioxidants [13].…”

Section: Introductionmentioning

confidence: 94%

“…In the agricultural area, electromagnetic-wave irradiation has been used to develop crop varieties, manage insect pests, monitor fertilizer efficiency, and preserve agricultural produce [4]. Effects of electromagnetic-wave irradiation on seed germination, seedling establishment, and crop productivity have been investigated in wheat [5], rice [6], maize [7], soybean [8], and sugar beet [9]. In rice, UV-B irradiation suppressed seedling growth during leaf development [6]; however, microwave irradiation of soils increased yields by 34% compared to the control [10].…”

Section: Introductionmentioning

confidence: 99%

“…Effects of electromagnetic-wave irradiation on seed germination, seedling establishment, and crop productivity have been investigated in wheat [5], rice [6], maize [7], soybean [8], and sugar beet [9]. In rice, UV-B irradiation suppressed seedling growth during leaf development [6]; however, microwave irradiation of soils increased yields by 34% compared to the control [10]. Influences of electromagnetic-wave irradiation on crops are dose dependent, where low doses induced fewer side effects compared with high doses that affected plant phenotype.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphophysiological and Proteomic Responses on Plants of Irradiation with Electromagnetic Waves

Zhong

Wang

et al. 2021

IJMS

View full text Add to dashboard Cite

Electromagnetic energy is the backbone of wireless communication systems, and its progressive use has resulted in impacts on a wide range of biological systems. The consequences of electromagnetic energy absorption on plants are insufficiently addressed. In the agricultural area, electromagnetic-wave irradiation has been used to develop crop varieties, manage insect pests, monitor fertilizer efficiency, and preserve agricultural produce. According to different frequencies and wavelengths, electromagnetic waves are typically divided into eight spectral bands, including audio waves, radio waves, microwaves, infrared, visible light, ultraviolet, X-rays, and gamma rays. In this review, among these electromagnetic waves, effects of millimeter waves, ultraviolet, and gamma rays on plants are outlined, and their response mechanisms in plants through proteomic approaches are summarized. Furthermore, remarkable advancements of irradiating plants with electromagnetic waves, especially ultraviolet, are addressed, which shed light on future research in the electromagnetic field.

show abstract

Diurnal changes in C N metabolism and response of rice seedlings to UV-B radiation

Cited by 10 publications

References 60 publications

Transcriptional Changes of Cell Wall Organization Genes and Soluble Carbohydrate Alteration during Leaf Blade Development of Rice Seedlings

Transcriptional Changes of Cell Wall Organization Genes and Soluble Carbohydrate Alteration during Leaf Blade Development of Rice Seedlings

β-Sitosterol differentially regulates key metabolites for growth improvement and stress tolerance in rice plants during prolonged UV-B stress

Morphophysiological and Proteomic Responses on Plants of Irradiation with Electromagnetic Waves

Contact Info

Product

Resources

About