Diverse sapovirus genotypes identified in children hospitalised with gastroenteritis in selected regions of South Africa

Murray, Tanya Y.; Nadan, Sandrama; Page, Nicola; Taylor, Maureen B.

doi:10.1016/j.jcv.2016.01.003

Cited by 23 publications

(34 citation statements)

References 38 publications

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“…SaV often showed high genetic diversity during its surveillance, and three of the four human SaV genogroups were detected in this study. During a SaV surveillance in South Africa, a total of 14 genotypes were discovered, including all four human genogroups . Although no SaV genotype has always been prevalent as a predominant genotype in the human population like GII.4 noroviruses, GI.1 and GI.2 are the most reported SaV genotypes .…”

Section: Discussionmentioning

confidence: 99%

“…GIV has only a single genotype GIV.1, and GV could be subdivided into two genotypes; GV.1 and GV.2 . A series of studies revealed high genetic diversity of human SaVs in patients suffering from acute gastroenteritis …”

Section: Introductionmentioning

confidence: 99%

“…17 A series of studies revealed high genetic diversity of human SaVs in patients suffering from acute gastroenteritis. 9,[21][22][23] The importance of SaVs as the cause of acute gastroenteritis has drawn more attention in recent years. SaV infection is also one of the major public health concerns that cause acute diarrhea in China.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence and genetic diversity of human sapovirus associated with sporadic acute gastroenteritis in South China from 2013 to 2017

Xue¹,

Cai²,

Zhang³

et al. 2019

Journal of Medical Virology

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Human sapovirus (SaV) is an important viral agent for acute diarrhea worldwide, but timely prevalence data of human SaV in South China are still lacking. In this study, a 4‐year surveillance was conducted to characterize the prevalence and genetic characteristics of the circulating SaV associated with sporadic diarrhea in South China. From November 2013 to October 2017, 569 fecal samples from patients with acute diarrhea were collected. SaV was detected in 11 samples with a positive rate of 1.93%. Three human genogroups of GI, GII, and GIV were identified, including five GI.1 strains, three GI.2 strains, one GI.3 strain, one GII.8 strain, and one GIV strain. Furthermore, multiple alignments of complete capsid protein VP1 genes of five local GI.1 strains and other available GI.1 strains in GenBank were performed. Average pairwise identities were calculated at 95.33% and 99.36% at nucleotide and amino acid levels, and only six variable amino acid sites were found during its 36‐years’ evolution process. GI.1 strains could be further phylogenetically divided into four clusters with an approximate temporal evolution pattern, and local strains belonged to Cluster‐d with other four strains from China and Japan. In summary, SaV was identified as an etiological agent responsible for sporadic gastroenteritis in Guangzhou with a low prevalence rate as in other Chinese cities, but its high genetic diversity suggested the necessity of continuous SaV surveillance in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and genetic diversity of human sapovirus associated with sporadic acute gastroenteritis in South China from 2013 to 2017

Xue¹,

Cai²,

Zhang³

et al. 2019

Journal of Medical Virology

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“…The role of NoVs and SaVs as causative agents of gastroenteritis and their diversity in Africa is not well studied, except in some reports from a few African countries [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. There has been only one published study from Ethiopia regarding the presence and genetic diversity of NoVs [37], and none for SaVs to date.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular characterization of human noroviruses and sapoviruses in Ethiopia

et al. 2016

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Viral gastroenteritis is a major public health problem worldwide. In Ethiopia, very limited studies have been done on the epidemiology of enteropathogenic viruses. The aim of this study was to detect and characterize noroviruses (NoVs) and sapoviruses (SaVs) from acute gastroenteritis patients of all ages. Fecal samples were collected from diarrheic patients (n = 213) in five different health centers in Addis Ababa during June-September 2013. The samples were screened for caliciviruses by reverse transcription polymerase chain reaction (RT-PCR) using universal and genogroup-specific primer pairs. Phylogenetic analyses were conducted using the sequences of the PCR products. Of the clinical samples, 25.3 % and 4.2 % were positive for NoV and SaV RNA, respectively. Among the norovirus positives, 22 were sequenced further, and diverse norovirus strains were identified: GI (n = 4), GII (n = 17) and GIV (n = 1). Most strains were GII (n = 17/22: 77.2 %), which were further divided into three different genotypes (GII.4, GII.12/GII.g recombinant-like and GII.17), with GII.17 being the dominant (7/17) strain detected. GI noroviruses, in particular GI.4 (n = 1), GI.5 (n = 2) and GI.8 (n = 1), were also detected and characterized. The GIV strain detected is the first from East Africa. The sapoviruses sequenced were also the first reported from Ethiopia. Collectively, this study showed the high burden and diversity of noroviruses and circulation of sapoviruses in diarrheic patients in Ethiopia. Continued surveillance to assess their association with diarrhea is needed to define their epidemiology, disease burden, and impact on public health.

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“…In addition, genotype GII.1 presents a high prevalence compared to other SaV genotypes. 34,35 . The first description of genotype GI.7 was made by analyzing all capsid proteins using universal primers for specific genogroups detected in samples from three prefectures (Chiba, Ehime, and Ishikawa) in Japan between 1999 and 2005 17 .…”

Section: Discussionmentioning

confidence: 99%

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

Costa

Siqueira

Portal

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Acute gastroenteritis (AGE) is one of the most common causes of morbidity and mortality, especially among children from developing countries. Human adenovirus (HAdV) and sapovirus (SaV) are among the agents that cause AGE. The present study aimed to detect and genotype HAdV and SaV in 172 fecal samples from children with AGE, collected during a surveillance study carried out in a low-income community in Belém, Pará, between 1990 and 1992. Methods: HAdV was detected by nested PCR, using primers Hex1deg/Hex2deg and NeHex3deg/NeHex4deg. SaV was assayed by reverse transcription PCR (RT-PCR), nested PCR, and quantitative PCR. The nucleotide sequence was determined by direct cycle sequencing. Results: Overall, 43% (74/172) of samples were positive for HAdV, of which 70.3% (52/74) were sequenced and classified as belonging to five different species, mostly A and F. For SaV, positivity was 5.2% (9/172) and genotypes GI.1, GI.7, GII.1, and GV.2 were detected. Conclusions: The present results reinforce the need for further studies to obtain epidemiological data about the circulation of these viruses in Brazil, especially in the Amazon Region, where data from the early 1990's are scarce. Furthermore, the study describes for the first time the detection of SaV genotypes GI.7 and GV.2 in Brazil, showing that these types circulated in the region more than 25 years ago.

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Diverse sapovirus genotypes identified in children hospitalised with gastroenteritis in selected regions of South Africa

Abstract: Background: Sapoviruses (SaVs) are recognised as causative agents of gastroenteritis worldwide.

Cited by 23 publications

References 38 publications

Prevalence and genetic diversity of human sapovirus associated with sporadic acute gastroenteritis in South China from 2013 to 2017

Prevalence and genetic diversity of human sapovirus associated with sporadic acute gastroenteritis in South China from 2013 to 2017

Prevalence and molecular characterization of human noroviruses and sapoviruses in Ethiopia

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

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