Diversity and Effectivity of Indigenous Mesorhizobium Strains for Chickpea (Cicer arietinum L.) in Myanmar

Abstract

Chickpea (Cicer arietinum L.) is one of the world's main leguminous crops that provide chief source of food for humans. In the present study, we characterized thirty isolates of indigenous chickpea rhizobia from Myanmar based on the sequence analysis of the bacterial 16S rRNA gene. The sequence analysis confirmed that all isolates were categorized and identified as the genus Mesorhizobium and they were conspecific with M. plurifarium, M. muliense, M. tianshanense, and M. sp. This is the first report describing M. muliense, M. tianshanense, and M. plurifurium from different geographical distribution of indigenous mesorhizobia of chickpea in Myanmar. In order to substitute the use of chemical fertilizers in legume production, there is a need for the production of Biofertilizers with rhizobial inoculants. The effectiveness of Myanmar Mesorhizobim strains isolated from soil samples of major chickpea growing areas of Myanmar for plant growth and nitrogen fixation were studied in pot experiments. The nodule dry weight and acetylene reduction activity of the plant inoculated with Mesorhizobium tianshanense SalCP19 was significantly higher than the other tested isolates in Yezin-4 chickpea variety. But, Mesorhizobium sp. SalCP17 was showed high level of acetylene reduction activity per plant in Yezin-6 chickpea variety.

Mitigation of Salinity Stress on Chickpea Germination by Salicylic Acid Priming

“…Due to its highly nutritious composition which includes carbohydrate (54-71%), protein (13-31%), vitamins, phosphorus and physiologically active ingredients, chickpea constitutes a substantial step of essential nutrients in many regions (Xing et al, 2020). Also, chickpea, similar to other grain legumes, is an important part of rotation systems (Francis et al, 1986;Ceritoglu and Erman, 2019) due to its high adaptability (Gan et al, 2009;Soysal et al, 2020) and nitrogen fixation that enhances N content and productivity of soil (Soe et al, 2020). Some abiotic and biotic stresses reduced chickpea productivity up to 1 ton ha -1 although it has the potential to achieve 3.5-4 ton ha -1 under optimum growth conditions (Roorkiwal et al, 2018).…”

Exploring the Impact of Tea (Camellia sinensis (L.) O. Ktze.)/Trachelospermum jasminoides (Lindl.) Lem. Intercropping on Soil Health and Microbial Communities

“…Notably, Burkholderia is recognized as a plant growth bacterium that typically harbors the nifH gene, crucial to plant nitrogen fixation [56,57]. Mesorhizobium, well known for its nitrogen-fixing abilities, is commonly found in root nodules, forming a highly synergistic relationship with plant roots [58]. Additionally, Gemmatimonas has been shown in numerous studies to possess significant capabilities in nitrogen transformation and fixation [59,60].…”

Conservation tillage regulates the assembly, network structure and ecological function of the soil bacterial community in black soils

“…In brief, the representative sequences of OTU3445 and OTU2114 had 97.12% and 99.53% similarity to Burkholderia singularis and Mesorhizobium gobiense, respectively. Burkholderia and Mesorhizobium have been widely reported to have nitrogen xation capabilities (Fan et al, 2019;Soe et al, 2020), and their abundances were signi cantly higher in the rhizosphere soils with NT and RT than in those with MP. OTU2806 (Nitrosospira multiformis) and OTU3942 (Nitrosospira tenuis), which play a pivotal role in the nitri cation process (Lin et al, 2018), were enriched in the bulk soils with RT and rhizosphere soils with NT.…”