Diversity of bats trypanosomes in hydroeletric area of Belo Monte in Brazilian Amazonia

Costa, Andréa Pereira da; Nunes, Pablo Henrique; Leite, Beatriz Helena Santos; Ferreira, Juliana Isabel Giuli da Silva; Tonhosolo, Renata; Rosa, Adriana Rückert da; Rocha, Patrício Adriano da; Aires, Caroline C.; Gennari, Solange Maria; Marcili, Arlei

doi:10.1016/j.actatropica.2016.08.033

Cited by 19 publications

(21 citation statements)

References 37 publications

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“…However, in the present study, all the positive samples were isolated and cryopreserved (100% isolation success), while in other studies 20 a 35% of positive cultures did not generated isolates in several Brazilian biomes (CAVAZZANA et al, 2010;MARCILI et al, 2013;ACOSTA et al, 2014;COSTA et al, 2015COSTA et al, , 2016.…”

Section: Discussioncontrasting

confidence: 58%

“…Trypanosoma dionisii has been detected in the bat families Phyllostomidae, Molossidae, Vespertilionidae and Noctilionidae in all biomes analyzed, from northern to southern Brazil, while T. cruzi marinkellei has been found in phyllostomid and vespertilionid bats in northern, central and southeastern areas and T. cruzi in the bat families Phyllostomidae, Vespertilionidae, Noctilionidae and Thyropteridae in the Amazon, Pantanal and Atlantic rainforest biomes (COSTA et al, 2016(COSTA et al, , 2015ACOSTA et al, 2014;MARCILI et al, 2009a;CAVAZZANA et al, 2010). Additionally, Trypanosoma erneyi has been described in bats in Africa (LIMA et al, 2012).…”

Section: Introductionmentioning

confidence: 95%

“…The blood samples were collected by means of heart puncture and were inoculated into vacutainer tubes containing a biphasic medium (15% sheep red blood cells with 4% blood agar base) and liquid LIT medium supplemented with 20% FBS (COSTA et al, 2016). The culture was incubated at 28 °C, and the isolates were cryopreserved in liquid nitrogen in the Brazilian Trypanosomatid Collection (Coleção Brasileira de Tripanossomatídeos, CBT), in the Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Brazil.…”

Section: Isolation Of Bat Trypanosomesmentioning

confidence: 99%

“…New species of Trypanosoma were described recently in the bat genus Pteronotus in the state of Rondônia and in blood from bats in Pará (LIMA et al, 2015). In Altamira, T. cruzi, T. cruzi marinkellei and T. wauwau were isolated (COSTA et al, 2016). The Tapajós-Arapiuns Resex is a protected area designated for sustainable use of natural resources with difficult access with humans inhabit small villages inside the Amazon rainforest, with close contact with bats and limited information regarding trypanosome infection.…”

Section: Introductionmentioning

confidence: 99%

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Bat trypanosomes from Tapajós-Arapiuns Extractive Reserve in Brazilian Amazon

Szpeiter

Ferreira

Assis

et al. 2017

Rev. Bras. Parasitol. Vet.

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Trypanosoma comprises flagellates able to infect several mammalian species and is transmitted by several groups of invertebrates. The order Chiroptera can be infected by the subgenera Herpetosoma, Schizotrypanum, Megatrypanum and Trypanozoon. In this study, we described the diversity of bat trypanosomes and inferred phylogenetic relationships among the trypanosomes from bats caught in Tapajós-Arapiuns Extractive Reserve (Resex) in Pará state. Trypanosomes from bats were isolated by means of hemoculture, and the molecular phylogeny was based on the trypanosome barcode (SSUrDNA V7V8 variable region). A total of 111 bats were caught in the area, belonging to three families (Emballonuridae, Molossidae and Phyllostomidae) and 12 species. The bat trypanosome prevalence, as evaluated through hemoculture, was 9% all positive cultures were cryopreserve (100% of isolation success). Phylogenetic trees grouped nine isolates in T. cruzi marinkellei branch and only one in T. dionisii branch. Studies on bat trypanosome diversity are important for identifying pathogenic species and for generating support for control measures, especially in such areas where humans inhabit the forest with close contact with bat species. In addition, this is the first study in Resex Tapajós-Arapiuns extractive reserve and further studies should be conducted to elucidate the role of these parasites as environmental degradation biomarkers.Keywords: Trypanosoma, Chiroptera, Amazon, phylogeny, taxonomy. ResumoTrypanosoma compreende flagelados capazes de infectar diversas espécies de mamíferos e são transmitidos por diferentes grupos de invertebrados. A ordem Chiroptera pode ser parasitada pelos subgêneros Herpetosoma, Schizotrypanum, Megatrypanum e Trypanozoon. Neste estudo é descrita a diversidade de tripanossomas de morcegos capturados na Reserva Extrativista Tapajós-Arapiuns, no Estado do Pará. Os tripanossomas de morcegos foram isolados através de hemocultura e os estudos filogenéticos baseados na região de barcode de tripanossomas (SSUrDNA V7V8 região variável). Foram capturados 111 morcegos pertencentes a três famílias (Emballonuridae, Molossidae e Phyllostomidae) e 12 espécies. A prevalência dos tripanossomas de morcegos, avaliada por hemocultura, foi de 9% para as culturas positivas e todas foram criopreservadas (100% de eficiência no isolamento). As árvores filogenéticas agruparam nove isolados no ramo de T. cruzi marinkellei e um único isolado de T. dionisii. Estudos sobre a diversidade de tripanossomas de morcegos são importantes para identificar espécies patogênicas e gerar suporte para medidas de controle, principalmente em áreas silvestres com contato entre as populações humanas e de morcegos. Além disso, este foi o primeiro estudo realizado na Resex Tapajós-Arapiuns e novos estudos devem ser conduzidos para elucidar o papel destes parasitas como marcadores de degradação ambiental.Palavras-chave: Trypanosoma,

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Section: Discussioncontrasting

confidence: 58%

Section: Introductionmentioning

confidence: 95%

Section: Isolation Of Bat Trypanosomesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bat trypanosomes from Tapajós-Arapiuns Extractive Reserve in Brazilian Amazon

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Ferreira

Assis

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…are known to infect bats (da Costa et al, 2016). The significance of bats in the evolution of (human) parasitic trypanosomatids attracted great attention in the past years, because T. cruzi and related species of various mammalian orders were shown to evolve from bat trypanosomes, as a result of host switching (Hamilton et al, 2012).…”

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confidence: 99%

DNA of free-living bodonids (Euglenozoa: Kinetoplastea) in bat ectoparasites: potential relevance to the evolution of parasitic trypanosomatids

Szőke

Sándor

Boldogh

et al. 2017

Acta Veterinaria Hungarica

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Kinetoplastids are flagellated protozoa, including principally free-living bodonids and exclusively parasitic trypanosomatids. In the most species-rich genus, Trypanosoma, more than thirty species were found to infect bats worldwide. Bat trypanosomes are also known to have played a significant role in the evolution of T. cruzi, a species with high veterinary medical significance. Although preliminary data attested the occurrence of bat trypanosomes in Hungary, these were never sought for with molecular methods. Therefore, amplification of an approx. 900-bp fragment of the 18S rRNA gene of kinetoplastids was attempted from 307 ixodid and 299 argasid ticks collected from bats, and from 207 cimicid bugs collected from or near bats in Hungary and Romania. Three samples, one per each bat ectoparasite group, were PCR positive. Sequencing revealed the presence of DNA from free-living bodonids (Bodo saltans and neobodonids), but no trypanosomes were detected. The most likely source of bodonid DNA detected here in engorged bat ectoparasites is the blood of their bat hosts. However, how bodonids were acquired by bats, can only be speculated. Bats are known to drink from

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Kinetoplastids and Bats

2017

Bats and Human Health

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Diversity of bats trypanosomes in hydroeletric area of Belo Monte in Brazilian Amazonia

Cited by 19 publications

References 37 publications

Bat trypanosomes from Tapajós-Arapiuns Extractive Reserve in Brazilian Amazon

Bat trypanosomes from Tapajós-Arapiuns Extractive Reserve in Brazilian Amazon

DNA of free-living bodonids (Euglenozoa: Kinetoplastea) in bat ectoparasites: potential relevance to the evolution of parasitic trypanosomatids

Kinetoplastids and Bats

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