Diversity of Salmonella isolates and their distribution in a pig slaughterhouse in Huaian, China

Zhou, Zihao; Li, Jingwen; Zheng, Huaiping; Jin, Xuanchen; Shen, Yang; Lei, Tianyao; Sun, Xinyu; Pan, Zhiming; Jiao, Xinan

doi:10.1016/j.foodcont.2017.02.064

Cited by 37 publications

(25 citation statements)

References 31 publications

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“…Among the isolates, the prevalence of Salmonella was 27.83% (64/230) in ileum samples, 8.43% (14/166) in liver samples and 3.17% (2/63) in feces samples, respectively. The prevalence of Salmonella from ileum was slightly higher than the 23.8% observed in Brazil (da Silva et al 2012) and lower than the 36.5% reported in Huaian in China (Zhou et al 2017).The serotyping results indicated that S. Derby (43.75%) in B group was the predominant serovar in the slaughterhouse and processing chain, which is consistent with previous studies (Bonardi et al 2016;Piras et al 2011). In fact, S. Derby has been shown to be the most common serovar across the world.…”

Section: Discussionsupporting

confidence: 87%

“…For example, S. Derby has been shown to represent the most significant proportion of serovars in both pork and slaughterhouse in China (Cai et al 2016;Li et al 2014b). In general consistency with the reports by Zhou et al (Zhou et al 2017), we observed that S. Rissen was the second most common serovar in the slaughterhouse. A total of 14 Salmonella strains were isolated from liver samples consisting of S. Derby (n = 11), S. Chester (n = 2) and S. Typhimurium (n = 1).…”

Section: Discussionsupporting

confidence: 86%

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Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Yin

Zheng

et al. 2019

AMB Expr

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Salmonella is an important food-borne pathogen associated with public health and high economic losses. To investigate the prevalence and the characteristics of Salmonella in a pig slaughterhouse in Yangzhou, a total of 80 Salmonella isolates were isolated from 459 (17.43%) samples in 2016-2017. S. Derby (35/80, 43.75%) was the most prevalent, followed by S. Rissen (16/80, 20.00%) and S. Newlands (11/80, 13.75%). The highest rates of susceptibility were observed to cefoxitin (80/80, 100.0%) and amikacin (80/80, 100.0%), followed by aztreonam (79/80, 98.75%) and nitrofurantoin (79/80, 98.75%). The highest resistance rate was detected for tetracycline (65/80, 81.25%), followed by ampicillin (60/80, 75.00%), bactrim (55/80, 68.75%), and sulfisoxazole (54/80, 67.50%). Overall, 91.25% (73/80) of the isolates were resistant to at least one antibiotic, while 71.25% (57/80) of the isolate strains were multidrug resistant in the antimicrobial susceptibility tested. In addition, 86.36% (19/22) of the 22 antimicrobial resistance genes in the isolates were identified. Our data indicated that the resistance to certain antimicrobials was significantly associated, in part, with antimicrobial resistance genes. Furthermore, 81.25% (65/80) isolates harbored the virulence gene of mogA, of which 2 Salmonella Typhimurium isolates carried the mogA, spvB and spvC virulence genes at the same time. The results showed that swine products in the slaughterhouse were contaminated with multidrug resistant Salmonella commonly, especially some isolates carry the spv virulence genes. The virulence genes might facilitate the dissemination of the resistance genes to consumers along the production chain, suggesting the importance of controlling Salmonella during slaughter for public health.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 86%

Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Yin

Zheng

et al. 2019

AMB Expr

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“…The isolation of Salmonella was performed as previously described (9). In brief, 100 ml of buffered peptone water (BPW) was added to cotton swab samples and incubated at 37 • C overnight.…”

Section: Sample Collection and Salmonella Isolatedmentioning

confidence: 99%

“…Slaughter is considered as an important step causing Salmonella contamination in meat products (6,7). A study demonstrated that the total isolation rate of Salmonella was 34.0% in a pig slaughterhouse in Hainan, China, and cross-contamination was also observed during the slaughtering process (9). In northern Italy, Salmonella was found in 12.3 and 11.2% of carcass samples from two pig slaughterhouses, respectively, indicating the potential transmission of Salmonella from slaughterhouse to retail meat (10).…”

mentioning

confidence: 99%

Prevalence of Salmonella Isolates and Their Distribution Based on Whole-Genome Sequence in a Chicken Slaughterhouse in Jiangsu, China

Wang

Tian

et al. 2020

Front. Vet. Sci.

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“…Naturally contaminated samples were collected from feces and floors of a commercial chicken farm in Yangzhou, China according to the method ISO 6579-1:2017. The indigenous Salmonella strains were isolated as described ( Cai et al, 2016 ; Zhou et al, 2017 ). In brief, all samples were cultured in buffered peptone water (50 mL; Difco, BD, Sparks, MD, United States) for pre-enrichment at 37°C for 24 h. The bacterial culture (0.1 mL) was subsequently inoculated into Rappaport-Vassiliadis R10 broth (10 mL; Difco, BD) and cultured at 42°C for 24 h. Then the bacteria broth was streaked on xylose lysine tergitol 4 agar plates (Difco, BD) and incubated continuously at 37°C for 20–24 h. The presumptive Salmonella colonies on all plates were individually collected and the biochemical tests were performed based on an API 20E assay system (BioMerieux, Marcy l’Etoile, France).…”

Section: Methodsmentioning

confidence: 99%

Identification and Discrimination of Salmonella enterica Serovar Gallinarum Biovars Pullorum and Gallinarum Based on a One-Step Multiplex PCR Assay

2018

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Salmonella enterica serovar Gallinarum biovars Pullorum (S. Pullorum) and Gallinarum (S. Gallinarum) can result in pullorum disease and fowl typhoid in avian species, respectively, and cause considerable economic losses in poultry in many developing countries. Conventional Salmonella serotyping is a time-consuming, labor-intensive and expensive process, and the two biovars cannot be distinguished using the traditional serological method. In this study, we developed a rapid and reliable one-step multiplex polymerase chain reaction (PCR) assay to simultaneously identify and discriminate the biovars Pullorum and Gallinarum. The multiplex PCR method focused on three specific genes, stn, I137_08605 and ratA. Based on bioinformatics analysis, we found that gene I137_08605 was present only in S. Pullorum and S. Gallinarum, and a region of difference in ratA was deleted only in S. Pullorum after comparison with that of S. Gallinarum and other Salmonella serovars. Three pairs of primers specific for the three genes were designed for the multiplex PCR system and their selectivity and sensitivity were determined. The multiplex PCR results showed that S. Pullorum and S. Gallinarum could be identified and discriminated accurately from all tested strains including 124 strains of various Salmonella serovars and 42 strains of different non-Salmonella pathogens. In addition, this multiplex PCR assay could detect a minimum genomic DNA concentration of 67.4 pg/μL, and 100 colony forming units. The efficiency of the multiplex PCR was evaluated by detecting natural-occurring Salmonella isolates from a chicken farm. The results demonstrated that the established multiplex PCR was able to identify S. Gallinarum and S. Pullorum individually, with results being consistent with traditional serotyping and biochemical testing. These results demonstrated that a highly accurate and simple biovar-specific multiplex PCR assay could be performed for the rapid identification and discrimination of Salmonella biovars Gallinarum and Pullorum, which will be useful, particularly under massive screening situations.

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Diversity of Salmonella isolates and their distribution in a pig slaughterhouse in Huaian, China

Cited by 37 publications

References 31 publications

Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Prevalence of Salmonella Isolates and Their Distribution Based on Whole-Genome Sequence in a Chicken Slaughterhouse in Jiangsu, China

Identification and Discrimination of Salmonella enterica Serovar Gallinarum Biovars Pullorum and Gallinarum Based on a One-Step Multiplex PCR Assay

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