DNA analysis in charred grains of naked wheat from several archaeological sites in Spain

Phylogenetic and population structural inference from genomic ancestry maintained in present‐day common wheat Chinese landraces

“…DNA extraction from archeological, especially paleobotanical, plant specimens and further high‐throughput sequencing have been accomplished using different protocols (Kistler and Shapiro, ; Li et al ., ; Kistler, ; Fernández et al ., ). In our case, we developed a pipeline integrating the optimized PTB ( N ‐phenacylthiazolium bromide) and guanidine hydrochloride DNA extraction from single wheat seeds and subsequent compatible whole‐genome shotgun sequencing with Illumina HiSeq X10.…”

“…Notably, the M29‐12‐2 and M29‐12‐3 samples generated endogenous rates similar to the mixed sample in both total mapping rate and unique mapping rate. In comparison with the respective mapping rates in published ancient plant samples, the high total mapping and unique mapping rates of our single‐seed samples indicated the sufficient endogenous content of DNA reads generated by current single‐seed DNA extraction and sequencing methods (Kistler and Shapiro, ; Kistler, ; Fernández et al ., ).…”

Exploring Fennoscandian agricultural history through genetic analysis of aged crop materials

“…Nuclear regions have also been the target for traditional PCR-based analyses. The wheat high molecular weight (HMW) glutenin alleles have been used to evaluate sample quality and discriminate between tetraploid and hexaploid wheat species (Brown, et al, 1994;Oliveira, et al, 2012b;Fernández, et al, 2013). In maize, multiple nuclear regions have been targeted in order to study the early history of maize domestication and its subsequent dispersal (Goloubinoff, et al, 1993;Freitas, et al, 2003;Jaenicke-Després, et al, 2003;Lia, et al, 2007).…”

Population genetic structure in Fennoscandian landrace rye (Secale cereale L.) spanning 350 years

“…Mascher et al 2016;Hagenblad et al 2017). In contrast, cereals preserved in a charred or waterlogged state has turned out to be notoriously difficult for DNA-studies (Fernández et al 2013;Nistelberger et al 2016;Lundström et al 2018). In Fennoscandia, the climate makes desiccated materials rare in typical archaeological contexts.…”