DNA aptamers targeting Leishmania infantum H3 protein as potential diagnostic tools

Frezza, Valerio; Pinto-Díez, Celia; Fernández, Gerónimo; Soto, Manuel; Martín, M. Elena; García‐Sacristán, Albino; González, Víctor

doi:10.1016/j.aca.2020.02.012

Cited by 22 publications

(15 citation statements)

References 36 publications

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“…ELONA assays were performed to study the enrichment of the populations obtained after each round of selection and the affinity for the target of the individual aptamers as described by Frezza et al [ 52 ]. Aptamers were labeled by PCR using 5′ digoxigenin-labeled F5 or R5 primers or digoxigenin-labeled aptamers purchased by IBA, respectively.…”

Section: Methodsmentioning

confidence: 99%

DNA Aptamers against Vaccinia-Related Kinase (VRK) 1 Block Proliferation in MCF7 Breast Cancer Cells

et al. 2021

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Vaccinia-related kinase (VRK) 1 is a serin/threonine kinase that plays an important role in DNA damage response (DDR), phosphorylating some proteins involved in this process such as 53BP1, NBS1 or H2AX, and in the cell cycle progression. In addition, VRK1 is overexpressed in many cancer types and its correlation with poor prognosis has been determined, showing VRK1 as a new therapeutic target in oncology. Using in vitro selection, high-affinity DNA aptamers to VRK1 were selected from a library of ssDNA. Selection was monitored using the enzyme-linked oligonucleotide assay (ELONA), and the selected aptamer population was cloned and sequenced. Three aptamers were selected and characterized. These aptamers recognized the protein kinase VRK1 with an affinity in the nanomolar range and showed a high sensibility. Moreover, the treatment of the MCF7 breast cell line with these aptamers resulted in a decrease in cyclin D1 levels, and an inhibition of cell cycle progression by G1 phase arrest, which induced apoptosis in cells. These results suggest that these aptamers are specific inhibitors of VRK1 that might be developed as potential drugs for the treatment of cancer.

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Section: Methodsmentioning

confidence: 99%

DNA Aptamers against Vaccinia-Related Kinase (VRK) 1 Block Proliferation in MCF7 Breast Cancer Cells

et al. 2021

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“…Thus, the application of detections that require expensive equipment or complex laboratory sites is significantly limited in these areas. In this case, a deal of aptamer-based biosensors has been developed for the identification of parasites due to their low cost, simplicity, portability ( Lee et al, 2012 ; Singh et al, 2019a ; Singh et al, 2019b ; Frezza et al, 2020 ; Minopoli et al, 2020 ). Take the diagnosis of malaria as an example, Singh et al established instrument-based and instrument-free approaches for pan malaria and P. falciparum species based on aptamers specific to Plasmodium lactate dehydrogenase (PLDH) and Plasmodium falciparum glutamate dehydrogenase (P f GDH) respectively ( Singh et al, 2019a ).…”

Section: Application Of Aptamers Biosensor In Infectious Diseases Diagnosismentioning

confidence: 99%

Systematic Evolution of Ligands by Exponential Enrichment Technologies and Aptamer-Based Applications: Recent Progress and Challenges in Precision Medicine of Infectious Diseases

Xin

Zhou

et al. 2021

Front. Bioeng. Biotechnol.

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Infectious diseases are considered as a pressing challenge to global public health. Accurate and rapid diagnostics tools for early recognition of the pathogen, as well as individualized precision therapy are essential for controlling the spread of infectious diseases. Aptamers, which were screened by systematic evolution of ligands by exponential enrichment (SELEX), can bind to targets with high affinity and specificity so that have exciting potential in both diagnosis and treatment of infectious diseases. In this review, we provide a comprehensive overview of the latest development of SELEX technology and focus on the applications of aptamer-based technologies in infectious diseases, such as targeted drug-delivery, treatments and biosensors for diagnosing. The challenges and the future development in this field of clinical application will also be discussed.

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“…They labeled either of the two aptamers with digoxigenin for Li H3 binding and used an HRP-labeled anti-digoxigenin antibody for detection. Their results indicated that these two DNA aptamers are promising biosensing molecules for leishmaniasis diagnosis 227 .…”

Section: Potential Utility Of Aptamer Technology For Pathogen Detectionmentioning

confidence: 99%

Oligonucleotide aptamers for pathogen detection and infectious disease control

Wan

Liu

2021

Theranostics

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During an epidemic or pandemic, the primary task is to rapidly develop precise diagnostic approaches and effective therapeutics. Oligonucleotide aptamer-based pathogen detection assays and control therapeutics are promising, as aptamers that specifically recognize and block pathogens can be quickly developed and produced through simple chemical synthesis. This work reviews common aptamer-based diagnostic techniques for communicable diseases and summarizes currently available aptamers that target various pathogens, including the SARS-CoV-2 virus. Moreover, this review discusses how oligonucleotide aptamers might be leveraged to control pathogen propagation and improve host immune system responses. This review offers a comprehensive data source to the further develop aptamer-based diagnostics and therapeutics specific for infectious diseases.

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DNA aptamers targeting Leishmania infantum H3 protein as potential diagnostic tools

Cited by 22 publications

References 36 publications

DNA Aptamers against Vaccinia-Related Kinase (VRK) 1 Block Proliferation in MCF7 Breast Cancer Cells

DNA Aptamers against Vaccinia-Related Kinase (VRK) 1 Block Proliferation in MCF7 Breast Cancer Cells

Systematic Evolution of Ligands by Exponential Enrichment Technologies and Aptamer-Based Applications: Recent Progress and Challenges in Precision Medicine of Infectious Diseases

Oligonucleotide aptamers for pathogen detection and infectious disease control

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