DNA Associated with Circulating Exosomes as a Biomarker for Glioma

Vaidya, Manjusha; Sugaya, Kiminobu

doi:10.3390/genes11111276

Cited by 25 publications

(19 citation statements)

References 73 publications

(89 reference statements)

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“…KRAS mutation is also confirmed in exosomes of pancreatic ductal adenocarcinoma in the early stage and the late phase ( Allenson et al, 2017 ). The serum exosomes of glioma patients also carry the gDNA sequence with the same biological mutation as glioblastoma ( Wang et al, 2018 ; Vaidya and Sugaya, 2020 ). In addition, the corresponding driving mutation was also found in the exosomes of prostate cancer ( Lazaro-Ibanez et al, 2014 ).…”

Section: Exosome Genomic Dna As a Tool For Colorectal Cancer Liquid B...mentioning

confidence: 99%

Roles of Exosome Genomic DNA in Colorectal Cancer

Wang

2022

Front. Pharmacol.

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Exosomes are extracellular vesicles that mediate cell-to-cell communication. Bioactive substances such as DNA, RNA, lipids, and proteins are present in it, and they play an essential role in the pathogenesis of colorectal cancer (CRC). The role of RNA and protein in exosomes has been extensively studied. Exosome DNA has recently attracted the attention of a great deal of scientists. According to studies, exosome DNA mainly contains genomic DNA (gDNA) and mitochondrial DNA (mtDNA), of which exosome gDNA is widely used in liquid biopsy of CRC. It includes a variety of clinically relevant tumor-specific mutation genes. In addition to liquid biopsy, researchers find that exosome gDNA regulates immune and metabolic functions in CRC, making it an important research object. However, the primary research on exosome gDNA is still limited. Here, we describe the occurrence and composition of exosomes. Summarize the essential characteristics and mode of action of exosome gDNA. Remarkably, this paper constitutes a comprehensive summary on the role of exosome gDNA on CRC with the intent of providing a theoretical basis and reference for early diagnosis and clinical treatment of cancer.

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Section: Exosome Genomic Dna As a Tool For Colorectal Cancer Liquid B...mentioning

confidence: 99%

Roles of Exosome Genomic DNA in Colorectal Cancer

Wang

2022

Front. Pharmacol.

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“…Exosomes also contain DNA molecules representing introns, non-coding genomic DNA, and other chromosomal sequences, including transposable elements. Several mutated genes specific to brain glioma have been found in exosomes, such as MGMT [22][23][24], PTEN [22], EGFR [25,26], and many others [27]. We aimed to verify whether the exoDNA concentration in the plasma of patients affected by glioma was related to the volume and histological grading of their tumors.…”

Section: Discussionmentioning

confidence: 99%

Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary Study

et al. 2022

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Glial neoplasms are a group of diseases with poor prognoses. Not all risk factors are known, and no screening tests are available. Only histology provides certain diagnosis. As already reported, DNA transported by exosomes can be an excellent source of information shared by cells locally or systemically. These vesicles seem to be one of the main mechanisms of tumor remote intercellular signaling used to induce immune deregulation, apoptosis, and both phenotypic and genotypic modifications. In this study, we evaluated the exosomal DNA (exoDNA) concentration in blood samples of patients affected by cerebral glioma and correlated it with histological and radiological characteristics of tumors. From 14 patients with diagnosed primary or recurrent glioma, we obtained MRI imaging data, histological data, and preoperative blood samples that were used to extract circulating exosomal DNA, which we then quantified. Our results demonstrate a relationship between the amount of circulating exosomal DNA and tumor volume, and mitotic activity. In particular, a high concentration of exoDNA was noted in low-grade gliomas. Our results suggest a possible role of exoDNAs in the diagnosis of brain glioma. They could be particularly useful in detecting early recurrent high-grade gliomas and asymptomatic low-grade gliomas.

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“…Tumor biomarker is a term used to describe a potential indicator of cancer development and progression specific to a particular cancer type, like proteins, nucleic acids (DNA, mRNA, miRNA), and EVs [ 25 , 34 ]. Ideally, a new biomarker should fulfill an unmet need in cancer detection; alternatively, it should provide advantages over preexisting biomarker(s), such as being more accurate and simpler to measure, faster, or cheaper [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs and Extracellular Vesicles as Distinctive Biomarkers of Precocious and Advanced Stages of Breast Cancer Brain Metastases Development

Figueira

Godinho-Pereira

Galego

et al. 2021

IJMS

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Triple negative breast cancer presents higher mortality and poorer survival rates than other breast cancer (BC) types, due to the proneness to brain metastases formation, which are usually diagnosed at advanced stages. Therefore, the discovery of BC brain metastases (BCBM) biomarkers appears pivotal for a timely intervention. With this work, we aimed to disclose microRNAs (miRNAs) and extracellular vesicles (EVs) in the circulation as biomarkers of BCBM formation. Using a BCBM animal model, we analyzed EVs in plasma by nanoparticle tracking analysis and ascertained their blood-brain barrier (BBB) origin by flow cytometry. We further evaluated circulating miRNAs by RT-qPCR and their brain expression by in situ hybridization. In parallel, a cellular model of BCBM formation, combining triple negative BC cells and BBB endothelial cells, was used to differentiate the origin of biomarkers. Established metastases were associated with an increased content of circulating EVs, particularly of BBB origin. Interestingly, deregulated miRNAs in the circulation were observed prior to BCBM detection, and their brain origin was suggested by matching alterations in brain parenchyma. In vitro studies indicated that miR-194-5p and miR-205-5p are expressed and released by BC cells, endothelial cells and during their interaction. These results highlight miRNAs and EVs as biomarkers of BCBM in early and advanced stages, respectively.

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DNA Associated with Circulating Exosomes as a Biomarker for Glioma

Cited by 25 publications

References 73 publications

Roles of Exosome Genomic DNA in Colorectal Cancer

Roles of Exosome Genomic DNA in Colorectal Cancer

Circulating Exosomal-DNA in Glioma Patients: A Quantitative Study and Histopathological Correlations—A Preliminary Study

MicroRNAs and Extracellular Vesicles as Distinctive Biomarkers of Precocious and Advanced Stages of Breast Cancer Brain Metastases Development

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