DNA Barcode as an effective tool in the identification of billfishes (Scombroidei, Teleostei) from exported specimens

DNA Barcoding of Commercially Important Snappers (Genus Lutjanus (Pisces: Lutjanidae)) from Aceh Waters, Indonesia

“…The thermocycling conditions consisted of initial denaturation at 95℃ (2 min) followed by denaturation at 94℃ (30 cycles, 45 s), annealing at 49.7 -60℃ (45 s); elongation at 72℃ (1 min), and final extension at 72℃ (10 min) before termination of the reaction at 4℃ [9,15]. The FishF1 primer region is typically short enough to be easily amplified using this PCR method, and its sequencing is technically feasible and facilitates efficient DNA barcoding workflow [25].…”