DNA barcoding Chinese medicinal Bupleurum

Chao, Zhi; Zeng, Wenbin; Liao, Jing; Liu, Li; Liang, Zhenbiao; Li, X

doi:10.1016/j.phymed.2014.09.001

Cited by 26 publications

(22 citation statements)

References 30 publications

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“…Planch [ 42 ]. Rbc L and mat K have been proposed as plant barcodes by the CBOL Plant Working Group [ 25 ] and have also been used to discriminate species of diverse plant taxa and to clarify taxonomic origins [ 43 , 44 ] in many extensive taxonomic investigations. In our study, we found that ITS was to be the best candidate barcode among the five single loci ( Fig 2 ).…”

Section: Discussionmentioning

confidence: 99%

Potential DNA barcodes for Melilotus species based on five single loci and their combinations

Meng³

et al. 2017

PLoS ONE

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Melilotus, an annual or biennial herb, belongs to the tribe Trifolieae (Leguminosae) and consists of 19 species. As an important green manure crop, diverse Melilotus species have different values as feed and medicine. To identify different Melilotus species, we examined the efficiency of five candidate regions as barcodes, including the internal transcribed spacer (ITS) and two chloroplast loci, rbcL and matK, and two non-coding loci, trnH-psbA and trnL-F. In total, 198 individuals from 98 accessions representing 18 Melilotus species were sequenced for these five potential barcodes. Based on inter-specific divergence, we analysed sequences and confirmed that each candidate barcode was able to identify some of the 18 species. The resolution of a single barcode and its combinations ranged from 33.33% to 88.89%. Analysis of pairwise distances showed that matK+rbcL+trnL-F+trnH-psbA+ITS (MRTPI) had the greatest value and rbcL the least. Barcode gap values and similarity value analyses confirmed these trends. The results indicated that an ITS region, successfully identifying 13 of 18 species, was the most appropriate single barcode and that the combination of all five potential barcodes identified 16 of the 18 species. We conclude that MRTPI is the most effective tool for Melilotus species identification. Taking full advantage of the barcode system, a clear taxonomic relationship can be applied to identify Melilotus species and enhance their practical production.

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Section: Discussionmentioning

confidence: 99%

Potential DNA barcodes for Melilotus species based on five single loci and their combinations

Meng³

et al. 2017

PLoS ONE

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“…These candidates are widely used as genetic markers to identify plant species and to analyze phylogenetic relationships (Kato et al, 1998;Kress and Erickson, 2007;Saarela et al, 2013). Chloroplast matK and rbcL DNA barcode regions have been used to identify species of diverse plant taxa, including herbal materials, and to clarify taxonomic origins (Cabrera et al, 2008;Sucher and Carles, 2008;Guo et al, 2011;Chao et al, 2014). In particular, DNA barcoding can provide objective tools that permit the authentication and identification of herbal medicines, especially for medicines that are often sold as dried slices or powders.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences

Moon

Kim

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Methods to identify Pinelliae Tuber and Arisaematis Rhizoma are required because of frequent reciprocal substitution between these two herbal medicines and the existence of several closely related plant materials. As a result of the morphological similarity of dried tubers, correct discrimination of authentic herbal medicines is difficult by conventional methods. Therefore, we analyzed DNA barcode sequences to identify each herbal medicine and the common adulterants at a species level. To verify the identity of these herbal medicines, we collected five authentic species (Pinellia ternata for Pinelliae Tuber, and Arisaema amurense, A. amurense var. serratum, A. erubescens, and A. heterophyllum for Arisaematis Rhizoma) and six common adulterant plant species. Maturase K (matK) and ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) genes were then amplified using universal primers. In comparative analyses of two DNA barcode sequences, we obtained 45 species-specific nucleotides sufficient to identify each species (except A. erubescens with matK) and 28 marker nucleotides for each species (except P. pedatisecta with rbcL). Sequence differences at corresponding positions of the two combined DNA barcodes provided genetic marker nucleotides that could be used to identify specimens of the correct species among the analyzed medicinal plants. Furthermore, we generated a phylogenetic tree showing nine distinct groups depending on the species. These results can be used to authenticate Pinelliae Tuber and Arisaematis Rhizoma from their adulterants and to identify each species. Thus, comparative analyses of plant DNA barcode sequences identified useful genetic markers for the authentication of Pinelliae Tuber and Arisaematis Rhizoma from several adulterant herbal materials.

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“…Since its conception [12], DNA barcoding, as a reliable, cost-effective, and accessible solution for species identification, has been widely used [15,45–47]. The CBOL Plant Working Group proposed the combination of rbcL + matK as a plant barcode [48].…”

Section: Discussionmentioning

confidence: 99%

Re-establishment of species from synonymies based on DNA barcoding and phylogenetic analysis using Diplopterygium simulans (Gleicheniaceae) as an example

et al. 2017

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Because synonymy treatment traditionally relies on morphological judgments, it usually causes many problems in species delimitation and in the biodiversity catalogue. For example, Diplopterygium simulans, which belongs to the Gleicheniaceae family, has been considered to be synonymous with D. glaucum or D. giganteum based mainly on the morphology of its pinna rachis and blade. In the absence of molecular evidence, these revisions remain doubtful. DNA barcoding, which is considered to be a powerful method for species-level identification, was employed to assess the genetic distance among 9 members of the Diplopterygium genus. The results indicate that D. simulans is an independent species rather than a synonymy of D. glaucum or D. giganteum. Moreover, phylogenetic analysis uncovered the sisterhood of D. simulans and D. cantonense, which is supported by their geographical distributions and morphological traits. Incorrect synonymy treatment is prevalent in the characterization of biological diversity, and our study proposes a convenient and effective method for validating synonym treatments and discovering cryptic species.

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DNA barcoding Chinese medicinal Bupleurum

Cited by 26 publications

References 30 publications

Potential DNA barcodes for Melilotus species based on five single loci and their combinations

Potential DNA barcodes for Melilotus species based on five single loci and their combinations

Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences

Re-establishment of species from synonymies based on DNA barcoding and phylogenetic analysis using Diplopterygium simulans (Gleicheniaceae) as an example

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