DNA barcoding identification of IUCN Red listed threatened species in the genus Aquilaria (Thymelaeaceae) using machine learning approaches

Lin, Yanhong; Feng, Tingting; Dai, Jiangpeng; Liu, Qiaozhen; Cai, Yao; Kuang, Jiarong; Wang, Zhaoyu; Gao, Xiaoxia; Liu, Shaofeng; Zhu, Shuang

doi:10.1016/j.phytol.2023.04.007

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…Until now, molecular identification methods for agarwood have mainly focused on DNA barcode screening and its combinations, such as ITS2+trnL-trnF , ITS2+matK (Kang et al, 2018), ITS+matK, ITS+rbcL, ITS+trnL-trnF (Li et al, 2018;Lin et al, 2023), and psbJ-petA+trnT-trnL (Hishamuddin et al, 2023).…”

Section: Importance Of Traditional Chinese Medicinal Agarwood Species...mentioning

confidence: 99%

“…Therefore, it is of great importance to identify medicinal agarwood species from large amounts of agarwood. Some scholars have suggested ITS2+trnL-trnF , ITS2+matK (Kang et al, 2019), ITS+matK, ITS+rbcL, ITS+trnL-trnF (Li et al, 2019;Lin et al, 2023), and psbJ-petA+trnT-trnL (Hishamuddin et al, 2023) for the molecular identification of agarwood. However, the actual situation is not working well.…”

Section: Introductionmentioning

confidence: 99%

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Successful development of molecular diagnostic technology combining mini-barcoding and high-resolution melting for traditional Chinese medicine agarwood species based on single-nucleotide polymorphism in the chloroplast genome

Feng,

Liu,

Xie

et al. 2024

Front. Plant Sci.

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Agarwood is a valuable traditional medicine and fragrance. The production process is a typical injury-induced defense response. Currently, there are approximately 22 known species in the genus Aquilaria Lam., all of which can produce agarwood, whereas there are only two legal species of traditional Chinese medicinal agarwood, Aquilaria sinensis (Lour.) Spreng. and Aquilaria agallocha (Lour.) Roxb. The Taiwan herbal Pharmacopoeia of China stipulates that the medicinal agarwood species are A. sinensis and its relatives in the same genus. Moreover, there are five species of agarwood available for clinical medicinal use in Japan, including A. agallocha and A. sinensis, which are often confused with each other or used in a mixed way in the trade process. Therefore, accurate identification of traditional Chinese medicinal agarwood species is important to ensure the authenticity of traditional medicines and to guide the safety of clinical medication. In this study, 59 specific single-nucleotide polymorphism loci were screened and obtained from the chloroplast genomes of 12 species of the genus Aquilaria Lam. We established an identification method for traditional Chinese medicinal agarwood using mini-barcoding combined with high-resolution melting (HRM) and designed and validated 10 pairs of primers from the psbM-trnD, psbA, rps16, petN, ndhE-psaC, rps4, atpE, ycf1, rps15-trnN, and matK regions. The amplification products were all less than 200 bp, with a high success rate of amplification. The method was applied to successfully identify traditional Chinese medicinal agarwood species from commercial agarwood samples. Overall, the sensitivity of this method was sufficient to detect 1% of adulterants in medicinal agarwood products, proving that mini-barcoding HRM is a powerful and flexible tool. This method can be used as a fast and effective high-throughput method for authenticity testing of traditional Chinese medicinal agarwood and its raw materials containing agarwood-containing proprietary Chinese medicines and is recommended for industrial applications.

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Section: Importance Of Traditional Chinese Medicinal Agarwood Species...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Successful development of molecular diagnostic technology combining mini-barcoding and high-resolution melting for traditional Chinese medicine agarwood species based on single-nucleotide polymorphism in the chloroplast genome

Feng,

Liu,

Xie

et al. 2024

Front. Plant Sci.

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“…However, natural disasters and excessive exploitation have led to a considerable decrease in the natural populations of theAquilaria genus, as well as the number of A. crassna trees . Therefore, cultivation of agarwood trees is encouraged to reduce the harvest from wild populations.…”

Section: Introductionmentioning

confidence: 99%

Phytochemistry and Cytotoxic Activity of Aquilaria crassna Pericarp on MDA-MB-468 Cell Lines

Nguyen,

Pham,

Nguyen

et al. 2023

ACS Omega

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The extracts of Aquilaria crassna pericarp were investigated on the MDA-MB-468, a breast cancer cell line, at desired concentration (1–50 μg/mL). The results showed that the dichloromethane (DCM) extract exhibited the strongest toxicity and was carried out subsequently. A total of nine compounds were isolated from the DCM extract using column chromatography and recrystallization, of which their structures were determined. Intriguingly, in addition to the previously reported compounds, neocucurbitacin A, a cucurbitacin triterpenoid aglycone with a lactone in ring A, was reported for the first time in the Aquilaria genus. Among the isolated compounds, cucurbitacin E highly inhibited MDA-MB-468 cell growth in a dose-dependent manner. Owing to binding abilities with the SH2 domain in the molecular docking study, cucurbitacin E, neocucurbitan A, neocucurbitan B, and cucurbitacin E 2-O-β-d-glucopyranoside act as STAT3 inhibitors and are suitable for further research. This study suggests thatAquilaria crassnafruits could serve as a promising source of natural compounds with potential anticancer effects, particularly against breast cancer.

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“…Anti-in ammatory properties: Sesquiterpenes like β-agarofuran and aromadendrene have been found to exhibit anti-in ammatory effects, which may be bene cial in treating in ammation and related conditions (M. A. Eissa et al, 2021).2. Analgesic properties: Sesquiterpenes in agarwood oil, such as β-agarofuran and agarospirol, have been suggested to exhibit analgesic effects, potentially making them useful in traditional medicine for pain management (T Li et al, 2023;Lin et al, 2023)…”

mentioning

confidence: 99%

Examining the Trend in Agarwood Oil Studies for the 5 Recent Years (2019-2023): A Bibliometric Analysis

YUSOFF,

NURLAILA,

Huzir

et al. 2024

Preprint

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This bibliometric article investigates the burgeoning research landscape surrounding Agarwood oil, derived from the Aquilaria tree species. The historical and cultural significance of Agarwood, coupled with its diverse applications in perfumery, traditional medicine, and religious practices, has fueled a surge in scientific interest. However, a comprehensive understanding of the current state of Agarwood oil research is lacking. This study addresses this gap through a systematic review and bibliometric analysis of scholarly databases. By employing techniques such as co-citation analysis and keyword co-occurrence, the research aims to quantify patterns, relationships, and collaborations within the literature. The methodology involves a focused exploration of a specified timeframe to capture recent developments, with the anticipation that the outcomes will include the identification of key contributors, thematic clusters, and emerging trends in Agarwood oil research. The study is poised to contribute valuable insights for researchers, policymakers, and industry stakeholders, offering a holistic perspective on the interconnectedness of Agarwood oil research and guiding future exploration in this dynamic field.

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DNA barcoding identification of IUCN Red listed threatened species in the genus Aquilaria (Thymelaeaceae) using machine learning approaches

Cited by 6 publications

References 23 publications

Successful development of molecular diagnostic technology combining mini-barcoding and high-resolution melting for traditional Chinese medicine agarwood species based on single-nucleotide polymorphism in the chloroplast genome

Successful development of molecular diagnostic technology combining mini-barcoding and high-resolution melting for traditional Chinese medicine agarwood species based on single-nucleotide polymorphism in the chloroplast genome

Phytochemistry and Cytotoxic Activity of Aquilaria crassna Pericarp on MDA-MB-468 Cell Lines

Examining the Trend in Agarwood Oil Studies for the 5 Recent Years (2019-2023): A Bibliometric Analysis

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