DNA barcoding in Dorcadionini (Coleoptera, Cerambycidae) uncovers mitochondrial-morphological discordance and the hybridogenic origin of several subspecies

Dascălu, Maria-Magdalena; Caba, Florina-Georgiana; Fusu, Lucian

doi:10.1007/s13127-021-00531-x

Cited by 10 publications

(39 citation statements)

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“…forticornis (Figures S7 and S8). This is similar to the study of Dascălu et al (2021), where the 28S sequence was identical for two closely related Dorcadion species (see more below) making this marker too conservative for inferring possible hybridisation—though it was suggested that this gene can be used in combination with COI to clarify cases of mitochondrial capture or even to detect hybrids (Sonnenberg et al, 2007). In another study, the 28S gene showed no differentiation between two closely related Cerambyx species— Cerambyx cerdo Linnaeus, 1758 and Cerambyx welensii (Küster, 1845; Torres‐Vila & Bonal, 2019).…”

Section: Discussionsupporting

confidence: 83%

“…Consequently, introgression, which results in a complex and highly variable mixture of genes, differs from simple hybridisation that ends in a relatively even mixture of gene and allele frequencies of two parental species in the first generation. Dascălu et al (2021) found that a presumably first‐generation cerambycid hybrid of Dorcadion lugubre Kraatz, 1873 and Dorcadion lineatocolle Kraatz, 1873 clustered with one parent ( D . lineatocolle ) on the mitochondrial COI tree and an allele with each parent on the nuclear ITS2 tree.…”

Section: Discussionmentioning

confidence: 99%

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Integrative taxonomy tests possible hybridisation between Central Asian cerambycids (Coleoptera)

et al. 2022

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Hybridisation can lead to the formation of new evolutionary lineages and some described insect species may in fact be first‐generation hybrids of their parental species. Anoplistes forticornis and A. galusoi are two closely related cerambycid taxa endemic to Central Asia that exhibit a peculiar geographical distribution. Although the common diagnostic elytral pattern makes these beetles superficially easily distinguishable, there are marked resemblances in their morphology. By applying an integrative taxonomy approach, we verify the taxonomic status of A. forticornis and A. galusoi and test the possibility of hybridisation. The results of our comprehensive morphological examination and molecular analyses targeting mitochondrial (COI, 16S rRNA) and nuclear (arginine kinase, CAD, 28S rRNA) genes support the specific status of these taxa. In mtDNA, the putative hybrid specimen is more closely related to A. forticornis, which indicates that a female of this species bred with a male of A. galusoi. The supposed ecotypic variability (manifested in elytral pattern) is apparently not related to the topography or spatial structure of habitat. The phylogenetic hypothesis supports a peripatric scenario in which populations of the common ancestor had been separated for a significant period of time, first by the mountain ranges of Dzungarian Alatau (~11–10 Ma) and ultimately by the Ili River. The diverged taxa came into recent secondary contact following these isolation events. The methodology presented herein can be widely applied to identify first‐generation hybridisation in Coleoptera.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Integrative taxonomy tests possible hybridisation between Central Asian cerambycids (Coleoptera)

et al. 2022

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“…According to the chronogram of Ashman et al (2022), intrageneric diversification occurred during Neogene, similar to our results. According to the chronogram of Dascălu et al (2022), the beginning of speciation within the genera Dorcadion was around 9.8 mya. Considering all recent studies and the present study, the emergence time of the subfamily Lamiinae is Jurassic to early Cretaceous, and the current species primarily emerged during the Neogene period.…”

Section: Discussionmentioning

confidence: 99%

Time-scaled phylogenetic analysis of extant Lamiinae (Coleoptera, Cerambycidae) species of East of Marmara Basin, Türkiye, and their evolutionary similarity with the Eurasian congeners

Soydabaş-Ayoub

Uçkan

2022

Preprint

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Lamiinae is a tremendous subfamily of Cerambycidae, with around 20,000 members dispersed across continents. The knowledge of the evolutionary history of the subfamily is scarcely, and there are growing doubts about the phylogenetic relationships due to the recognised illusion caused by the convergence of the morphological characters. The present study contributes to the evolutionary history and phylogenetic relationships of the tribes Acanthocinini, Acanthoderini, Agapanthiini, Batocerini, Dorcadionini, Lamiini, Mesosini, Monochamini, Phytoeciini, Phrynetini, Pogonocherini (including Exocentrini) and Saperdini with Neighbor-Joining (NJ), Maximum Likelihood (ML) and time-scaled Bayesian analyses based on partial mitochondrial COI and 16S rRNA and nuclear 28S rRNA gene regions (2257 base pair alignment length). The most recent common ancestor (MRCA) of the taxa included in the analyses appeared during the Middle or Late Cretaceous, and the MRCAs of the closely related tribes emerged in Paleogene. The MRCA of Dorcadionini, Lamiini and Monochamini was younger than the common ancestors of the other close tribes. The hypothetical ML phylogram was consistent with the Bayesian chronogram in the proximity of Batocerini to Lamiini, Acanthocinini to Acanthoderini, Phyretrini to Pogonocherini, and Phytoeciini to Saperdini, in addition to the affiliation of Lamiini, Dorcadionini and Monochamini. At the COI-based NJ and ML gene trees, Paraleprodera and Lamia (Lamiini) were sisters to Imantocera (Gnomini), Oberea (Obereini) to Phytoecia (Phytoeciini), and Hippopsis (Agapanthiini) to Omosarotes (Acanthomerosternoplini). The present results support Dorcadionini Gnomini and Monochamini as synonyms of Lamiini; and Obereini and Phytoeciini of Saperdini. We suggest that the emergence of the living tribes included in this study was during Paleogene, and the intrageneric diversifications occurred in Cenozoic, mostly during Neogene.

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“…The CO1 gene was amplified using the standard primer pair LCO1480 and HCO2198 [ 39 ], which amplifies the DNA barcode region for animals [ 40 , 41 ]. In case of failed reactions, we used a pair of internal primers from Fusu and Polaszek [ 42 ] that amplify the standard barcode region from two overlapping fragments (mini-barcodes, [ 43 ]): a modified LCO1490, named LCO1490M (5′-CAACAAATCATAAAGATATTGG-3′), pairs with MChaR1 (5′-CCYGTTCCAAYAAATATTCT-3′), and MChaF1 (5′-CCTCGAATAAATAATATAAGATT-3′) pairs with HCO2198.…”

Section: Methodsmentioning

confidence: 99%

Revision of the World Species of Megaphragma Timberlake (Hymenoptera: Trichogrammatidae)

Polaszek

Fusu

Viggiani

et al. 2022

Insects

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Megaphragma species are important models for basic organismal research, and many are potential biological control agents. We present the first extensive revision of species of the genus Megaphragma based on morphological and molecular data. Our revision includes all previously described species, 6 of which are synonymized, and 22 of which are described here as new. We also provide the first key to all species of the genus and reconstruct their phylogeny based on 28S and CO1 molecular markers. The following species are synonymized with M. longiciliatum Subba Rao: M. aligarhensis Yousuf and Shafee syn. nov.; M. amalphitanum Viggiani syn. nov.; M. decochaetum Lin syn. nov.; M. magniclava Yousuf and Shafee syn. nov.; M. shimalianum Hayat syn. nov. M. anomalifuniculi Yuan and Lou syn. nov. is synonymized with M. polychaetum Lin. The following species are described as new: M. antecessor Polaszek and Fusu sp. nov.; M. breviclavum Polaszek and Fusu sp. nov.; M. chienleei Polaszek and Fusu sp. nov.; M. cockerilli Polaszek and Fusu sp. nov.; M. digitatum Polaszek and Fusu sp. nov.; M. fanenitrakely Polaszek and Fusu sp. nov.; M. funiculatum Fusu, Polaszek, and Viggiani sp. nov.; M. giraulti Viggiani, Fusu, and Polaszek sp. nov.; M. hansoni Polaszek, Fusu, and Viggiani sp. nov.; M. kinuthiae Polaszek, Fusu, and Viggiani sp. nov.; M. liui Polaszek and Fusu sp. nov.; M. momookherjeeae Polaszek and Fusu sp. nov.; M. nowickii Polaszek, Fusu, and Viggiani sp. nov.; M. noyesi Polaszek and Fusu sp. nov.; M. pintoi Viggiani sp. nov.; M. polilovi Polaszek, Fusu, and Viggiani sp. nov.; M. rivelloi Viggiani sp. nov.; M. tamoi Polaszek, Fusu, and Viggiani sp. nov.; M. tridens Fusu, and Polaszek sp. nov.; M. uniclavum Polaszek and Fusu sp. nov.; M. vanlentereni Polaszek and Fusu sp. nov.; M. viggianii Fusu, Polaszek, and Polilov sp. nov.

show abstract

DNA barcoding in Dorcadionini (Coleoptera, Cerambycidae) uncovers mitochondrial-morphological discordance and the hybridogenic origin of several subspecies

Cited by 10 publications

References 121 publications

Integrative taxonomy tests possible hybridisation between Central Asian cerambycids (Coleoptera)

Integrative taxonomy tests possible hybridisation between Central Asian cerambycids (Coleoptera)

Time-scaled phylogenetic analysis of extant Lamiinae (Coleoptera, Cerambycidae) species of East of Marmara Basin, Türkiye, and their evolutionary similarity with the Eurasian congeners

Revision of the World Species of Megaphragma Timberlake (Hymenoptera: Trichogrammatidae)

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