DNA barcoding of mayflies (Insecta: Ephemeroptera) from South India

Abstract

In this study, DNA barcodes were generated for 40 species belonging to 32 genera under 10 families of Ephemeroptera from South India. Nucleotide sequence divergences were calculated using the Kimura two-parameter distance model and a neighbour-joining analysis was performed to provide a graphic display of the patterns of divergence among the species. This study demonstrates that COI barcoding is effective in discriminating among the mayfly species of South India, and provides a reference library for their future molecular identification.

Integrative taxonomy of the genus Dudgeodes Sartori, 2008 (Insecta, Ephemeroptera, Teloganodidae) from the Philippines with description of new species and supplementary descriptions of Southeast Asian species

“…Second species delimitation method is Poisson Tree Processes (PTP) (Zhang et al 2013) using Maximum Likelihood (ML) tree inferred from MEGA X following best model Tamura-3 parameter+GI from AICc. Teloganodes kodai was chosen as the outgroup (Selvakumar et al 2016). Single PTP method (p value 0.001) and multiple PTP (mPTP) (Kapli et al 2017) with default settings available from the webserver was used.…”

Sparsorythus sescarorum, new species from Mindoro, Philippines (Ephemeroptera, Tricorythidae)

“…Ends of each partition were trimmed to receive a complete matrix of all sequences used. The corresponding fragment of a COI sequence of Sparsorythus gracilis and Sparsorythus buntawensis available from GenBank (Table 1 ; Batucan et al 2016 ; Selvakumar et al 2016 ) were included in the statistical parsimony analysis conducted with TCS 1.21 ( Clement et al 2000 ). The network connection limit was set manually to 1000 steps in order to keep sub-networks of different species connected and show their inter-specific genetic distance.…”

Contribution to the knowledge of Teloganodidae (Ephemeroptera, Ephemerelloidea) of India

“…GenBank accession numbers for newly derived sequences are given in Table 1, with the nomenclature of gene sequences following Chakrabarty et al (2013). Other used sequences of Teloganodidae were taken from Selvakumar et al (2016), Garces et al (2020), and GenBank (unpublished data). Indoganodes jobini Selvakumar, Sivaramakrishnan & Jacobus, 2014, Teloganodes sartorii Selvakumar, Sivaramakrishnan & Jacobus, 2014, and T. kodai Sartori, 2008 were chosen as the outgroup (Selvakumar et al 2016).…”