DNA barcoding of mosquitoes collected through a nationwide survey in 2011 and 2012 in Malawi, Southeast Africa

Maekawa, Yoshihide; Pemba, Dylo; Kumala, Justin; Gowelo, Steven; Higa, Yukiko; Futami, Kyoko; Sawabe, Kyoko; Tsuda, Yuichi

doi:10.1016/j.actatropica.2020.105742

Cited by 9 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This molecular technique is based on the nucleotide sequences of a standard short DNA fragment (~ 400–800 bp) with low intragroup genetic divergence but high intergroup genetic divergence 22 , 23 . The cytochrome c oxidase subunit I ( COI ) gene is widely used as the universal barcode locus for the identification of mosquito species 24 , 25 . Several studies have reported the success of DNA barcoding in solving the taxonomic problem of mosquito species identification in many countries 26 – 28 .…”

Section: Introductionmentioning

confidence: 99%

Geometric morphometrics versus DNA barcoding for the identification of malaria vectors Anopheles dirus and An. baimaii in the Thai-Cambodia border

Chaiphongpachara

Changbunjong

Sumruayphol

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Anopheles (Cellia) dirus Peyton & Harrison and Anopheles baimaii Sallum & Peyton are sibling species within the Dirus complex belonging to the Leucosphyrus group, and have been incriminated as primary vectors of malaria in Thailand. In the present study, DNA barcoding and geometric morphometrics were used to distinguish between An. dirus and An. baimaii in the international border areas, Trat Province, eastern Thailand. Our results revealed that DNA barcoding based on the cytochrome c oxidase subunit I gene could not be used to distinguish An. dirus from An. baimaii. The overlapping values between intra- and interspecific genetic divergence indicated no barcoding gap present for An. dirus and An. baimaii (ranging from 0 to 0.99%). However, the results of the geometric morphometric analysis based on the wing shape clearly distinguished An. dirus and An. baimaii, with 92.42% of specimens assigned to the correct species. We concluded that geometric morphometrics is an effective tool for the correct species identification of these two malaria vectors. Our findings could be used to make entomological surveillance information more accurate, leading to further effective mosquito control planning in Thailand and other countries in Southeast Asia.

show abstract

Section: Introductionmentioning

confidence: 99%

Geometric morphometrics versus DNA barcoding for the identification of malaria vectors Anopheles dirus and An. baimaii in the Thai-Cambodia border

Chaiphongpachara

Changbunjong

Sumruayphol

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…perexiguus and Cx. univittatus seem to coexist at least in the southwestern Iberian Peninsula (Bueno‐Marí et al., 2012; Mixão et al., 2016; Bravo‐Barriga et al., 2021; Rodríguez‐Alarcón et al., 2021) and in Malawi in Southeast Africa (Maekawa et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

West Nile virus in Spain: Forecasting the geographical distribution of risky areas with an ecological niche modelling approach

Cuervo

Artigas

Mas‐Coma

et al. 2021

Transbounding Emerging Dis

View full text Add to dashboard Cite

West Nile virus (WNV), a well-known emerging vector-borne arbovirus with a zoonotic life cycle, represents a threat to both public and animal health. Transmitted by ornithophilic mosquitoes, its transmission is difficult to predict and even more difficult to prevent. The massive and unprecedented number of human cases and equid outbreaks in Spain during 2020 interpellates for new approaches. For the first time, we present an integrate analysis from a niche perspective to provide an insight to the situation of West Nile disease (WND) in Spain. Our modelling approach benefits from the combined use of global occurrence records of outbreaks of WND in equids and of its two alleged main vectors in Spain, Culex pipiens and Cx. perexiguus. Maps of the climatic suitability for the presence of the two vectors species and for the circulation of WNV are provided. The main outcome of our study is a map delineating the areas under certain climatic risk of transmission. Our analyses indicate that the climatic risk of transmission of WND is medium in areas nearby the south Atlantic coastal area of the Cadiz Gulf and the Mediterranean coast, and high in southwestern Spain. The higher risk of transmission in the basins of the rivers Guadiana and Guadalquivir cannot be attributed exclusively to the local abundance of Cx. pipiens, but could be ascribed to the presence and abundance of Cx. perexiguus. Furthermore, this integrated analysis suggests that the WNV presents an ecological niche of its own, not fully overlapping the ones of its hosts or vector, and thus requiring particular environmental conditions to succeed in its infection cycle.

show abstract

“…CO1-barcoding, based on PCR with LCO1490 and HCO2198 primers according to Hébert et al [ 36 ] and Folmer et al [ 37 ], has been shown successful in identifying many mosquito species worldwide (e.g. [ 47 – 49 ]). Thus, this PCR approach has been used for routine identification of mosquitoes collected in Germany for many years [ 50 ], including attempts to genetically verify morphologically pre-identified Ae.…”

Section: Discussionmentioning

confidence: 99%

The invasive Korean bush mosquito Aedes koreicus (Diptera: Culicidae) in Germany as of 2020

2021

View full text Add to dashboard Cite

Background The Korean bush mosquito Aedes koreicus was recently reported to have established a population in western Germany (Wiesbaden) in 2016. The species is difficult to distinguish morphologically from its close relative, the invasive Japanese bush mosquito Ae. japonicus, which is already widely distributed in many parts of Germany, including the area colonised by Ae. koreicus. Genetic confirmation of morphologically identified “Ae. japonicus” collection material, however, had only been done exceptionally before the German Ae. koreicus population became known. Methods Dried archived “Ae. japonicus” specimens both from the municipality of Wiesbaden and from deliberately and randomly selected distribution sites all over Germany were re-examined morphologically and genetically for admixture by Ae. koreicus. Moreover, cemeteries in the greater Wiesbaden area were sampled in 2019 and 2020 to check for Ae. koreicus spread. Korean and Japanese bush mosquitoes submitted to the German citizen science mosquito monitoring scheme “Mueckenatlas” in 2019 and 2020 were also subjected to particularly thorough species identification. The ND4 DNA sequences generated in this study in the context of species identification were phylogenetically compared to respective GenBank entries of Ae. koreicus. As a by-product, several genetic markers were evaluated for their suitability to identify Ae. koreicus. Results Aedes koreicus specimens could be identified in mosquito collection material and submissions from Wiesbaden from 2015 onwards, suggesting establishment to have happened in the same year as Ae. japonicus establishment. Detections of Ae. koreicus from 2019 and 2020 in Wiesbaden indicate a negligible enlargement of the populated area as described for 2018. Two Ae. koreicus specimens were also submitted from the city of Munich, southern Germany, in 2019 but further specimens could not be identified during immediate local inspections. Comparison of ND4 sequences generated in this and other studies demonstrate a high degree of homology, suggesting that this DNA region is not informative enough for clarification of origins and relationships of Ae. koreicus populations. For genetic identification of Ae. koreicus, PCR primers used for classical CO1 barcoding were found to lead to mismatches and produce no or incorrect amplicons. Alternative CO1 primers or a validated ND4 marker should be used instead. Conclusions Aedes koreicus is probably introduced into Germany every now and then but rarely succeeds in becoming established. As with most European populations, the German population is characterised by a limited expansion tendency. Since Ae. koreicus is a potential vector, however, Asian bush mosquitoes found at new places should be examined quite carefully and known distribution areas of Ae. japonicus regularly checked for the presence of Ae. koreicus. Graphical Abstract

show abstract

DNA barcoding of mosquitoes collected through a nationwide survey in 2011 and 2012 in Malawi, Southeast Africa

Cited by 9 publications

References 27 publications

Geometric morphometrics versus DNA barcoding for the identification of malaria vectors Anopheles dirus and An. baimaii in the Thai-Cambodia border

Geometric morphometrics versus DNA barcoding for the identification of malaria vectors Anopheles dirus and An. baimaii in the Thai-Cambodia border

West Nile virus in Spain: Forecasting the geographical distribution of risky areas with an ecological niche modelling approach

The invasive Korean bush mosquito Aedes koreicus (Diptera: Culicidae) in Germany as of 2020

Contact Info

Product

Resources

About