DNA Barcoding of Mosquitoes from the Pantanos de Centla Biosphere Reserve, Southeastern Mexico

Ortega‐Morales, Aldo I.; Hernández‐Triana, Luis M.; Chan-Chablé, Rahuel J.; Garza-Hernández, Javier A.; González-Álvarez, Vicente Homero; Ruíz-Arrondo, Ignacio; Nikolova, Nadya; Martínez‐Arce, Arely; Fooks, Anthony R.; Rodríguez‐Pérez, Mario A.

doi:10.2987/21-6967

Cited by 2 publications

(4 citation statements)

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“…However, this method is prone to error, especially when identifying mosquitoes belonging to a species complex, as well as isomorphic species, cryptic species, and samples damaged during collection [ 12 ]. Therefore, several more advanced and accurate modern techniques have been used to identify mosquitoes, and these have greatly increased our knowledge and understanding of mosquito vectors [ 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. Culex pseudovishnui , Cx.…”

Section: Discussionmentioning

confidence: 99%

“…DNA barcoding is a highly effective molecular technique for the identification of mosquito species that are morphologically similar or indistinguishable [ 21 , 22 , 46 , 47 ]. Our genetic analysis and the ASAP method suggested that DNA barcoding based on COI sequences can be used to distinguish between Cx.…”

Section: Discussionmentioning

confidence: 99%

“…vishnui and the results were consistent with those of morphological identification. Analysis of the genetic distance between species shows that the species are separated by a DNA barcoding gap, which is the difference between the greatest intraspecific distance and the smallest interspecific distance [ 21 , 22 , 46 ]. Our results of DNA barcoding are consistent with those of a previous study in Sri Lanka that used DNA barcoding to successfully identify members in the Cx.…”

Section: Discussionmentioning

confidence: 99%

“…However, this method is limited by the need for complete morphological specimens and practitioner expertise [ 10 ]. Currently, effective alternative methods for species identification include geometric morphometrics and DNA barcoding; both may support the standard method to accurately identify mosquito samples that are morphologically similar [ 16 , 17 , 18 , 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Species Identification of the Major Japanese Encephalitis Vectors within the Culex vishnui Subgroup (Diptera: Culicidae) in Thailand Using Geometric Morphometrics and DNA Barcoding

Saiwichai

Laojun

Chaiphongpachara

et al. 2023

Insects

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Japanese encephalitis (JE) is a viral infection of the brain caused by the Japanese encephalitis virus, which spreads globally, particularly in 24 countries of Southeast Asia and the Western Pacific region. In Thailand, the primary vectors of JE are Cx. pseudovishnui, Cx. tritaeniorhynchus, and Cx. vishnui of the Cx. vishnui subgroup. The morphologies of three mosquito species are extremely similar, making identification challenging. Thus, geometric morphometrics (GM) and DNA barcoding were applied for species identification. The results of cross-validation reclassification revealed that the GM technique based on wing shape analysis had relatively high potential for distinguishing Cx. pseudovishnui, Cx. tritaeniorhynchus, and Cx. vishnui (total performance = 88.34% of correctly assigned individuals). While the DNA barcoding yielded excellent results in identifying these Culex species based on the DNA barcode gap (average intraspecific genetic distance = 0.78% ± 0.39% and average interspecific genetic distance = 6.14% ± 0.79%). However, in the absence of the required facilities for DNA barcoding, GM techniques can be employed in conjunction with morphological methods to enhance the reliability of species identification. Based on the results of this study, our approach can help guide efforts to identify members of the Cx. vishnui subgroup, which will be useful for the effective vector control of JE in Thailand.

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