DNA barcoding of selected alpine beetles with focus on Curculionoidea (Coleoptera)

Primo rilevamento e identificazione molecolare di Donus intermedius (Coleoptera: Curculionoidae) come parassita di Melissa officinalis in Alto Adige

“…The generated consensus and unique sequence of Donus intermedius was used as a query to The morphological identification of larval stadiums of Coleoptera represents a challenge since the known illustrations, descriptions, and comprehensive keys are not always available or many species are not included in the literature [6]. The molecular identification through DNA barcoding represents an important tool for the identification of Coleoptera [7] and can be used for larval stadiums. Here we present a unique consensus barcode sequence for the species Donus intermedius supported with taxonomic consistency that can be used for identification and discrimination within the genera at species level.…”

Delimiting species, revealing cryptic diversity, and population divergence in Qinghai‐Tibet Plateau weevils through DNA barcoding

“…The efficacy of this approach has been successfully demonstrated across various taxa of Coleoptera, including Curculionidae (Astrin et al., 2012 ; Ma et al., 2022 ; Yang et al., 2021 ), Coccinellidae (Huang et al., 2020 ), and Tenebrionidae (Li et al., 2020 ). Previous studies have also shown the effectiveness of DNA barcoding in species identification and diagnosis within the Curculionoidea (Germann et al., 2017 ; Ma et al., 2022 ). In addition to the identification of known species, COI barcodes have also been proven to be suitable for elucidating cryptic species (Craft et al., 2010 ; Janzen et al., 2017 ; Velona et al., 2015 ), unveiling biogeographic patterns (Chen et al., 2018 ; Dinca et al., 2021 ), exploring population genetics (Gomez et al., 2018 ; Lin et al., 2020 ), and reconstructing phylogenetic relationships (Cognato et al., 2020 ; Grebennikov, 2022 ; Hajibabaei et al., 2007 ) at the species level.…”