DNA barcoding, species-specific PCR for the identification of three stored-product pest species of genus Palorus (Coleoptera: Tenebrionidae)

Lu, Yujie; Zhao, Yaru; Wang, Zhengyan; Miao, Shiyuan; Liu, Fengjie; Emery, Robert N.

doi:10.1016/j.jspr.2018.06.001

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…However, due to the large number of leafhoppers and the small size of the Erythroneurini leafhoppers, generally about 2∼4 mm ( Dietrich & Dmitriev, 2006 ), they are difficult to identify. In recent years, the development of molecular technology has been applied to the classification of insects ( Singh et al, 2017 ; Lu et al, 2018 ; Matsuia et al, 2022 ) and to support the results and correct the attribution of traditional classification. Our findings here on the complete mitochondrial genome supports the classification of two new species.…”

Section: Discussionmentioning

confidence: 99%

Two new species of Erythroneurini (Hemiptera, Cicadellidae, Typhlocybinae) from southern China based on morphology and complete mitogenomes

Zhang,

Wang,

et al. 2024

PeerJ

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Erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) are utilized to resolve the relationship between the four erythroneurine leafhopper (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini): Arboridia (Arboridia) rongchangensis sp. nov., Thaia (Thaia) jiulongensis sp. nov., Mitjaevia bifurcata Luo, Song & Song, 2021 and Mitjaevia diana Luo, Song & Song, 2021, the two new species are described and illustrated. The mitochondrial gene sequences of these four species were determined to update the mitochondrial genome database of Erythroneurini. The mitochondrial genomes of four species shared high parallelism in nucleotide composition, base composition and gene order, comprising 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and an AT control region, which was consistent with majority of species in Cicadellidae; all genes revealed common trait of a positive AT skew and negative GC skew. The mitogenomes of four species were ultra-conservative in structure, and which isanalogous to that of others in size and A + T content. Phylogenetic trees based on the mitogenome data of these species and another 24 species were built employing the maximum likelihood and Bayesian inference methods. The results indicated that the four species belong to the tribe Erythroneurini, M. diana is the sister-group relationship of M. protuberanta + M. bifurcata. The two species Arboridia (Arboridia) rongchangensis sp. nov. and Thaia (Thaia) jiulongensis sp. nov. also have a relatively close genetic relationship with the genus Mitjaevia.

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Section: Discussionmentioning

confidence: 99%

Two new species of Erythroneurini (Hemiptera, Cicadellidae, Typhlocybinae) from southern China based on morphology and complete mitogenomes

Zhang,

Wang,

et al. 2024

PeerJ

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Section: Methodsmentioning

confidence: 99%

“…Total genomic DNA was extracted based on the method of Lu et al (2018) with the commercial Ezup Column Animal Genomic DNA Purification Kit (Sangon Biotech, China) and following the manufacturer’s protocol. The PCR primer and reaction conditions for the COI region followed Folmer et al (1994) and Lu et al (2018) . Products were sequenced in a single direction by Sangon (Shanghai) Co., Ltd. Sequences were edited and analyzed using the software Chromas version 2.3 and BioEdit 7.0.4.1 ( Hall 1999 ).…”

Section: Methodsmentioning

confidence: 99%

Revision of the Conwentzia Enderlein, 1905 (Neuroptera, Coniopterygidae) of China, combining morphological and molecular characters

Zhao¹,

Liu²,

Liu³

2021

Self Cite

1,320

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The Chinese species of Conwentzia Enderlein are revised by integrating morphological characters and molecular data. Conwentzia yunguiana Liu & Yang, 1993 is proposed as a junior synonym of Conwentzia nietoi Monserrat, 1982, syn. nov. and Conwentzia orthotibia Yang, 1974 is proposed as a junior synonym of Conwentzia pineticola Enderlein, 1905, syn. nov. Moreover, a key to the adult males of the Conwentzia from China and DNA barcodes are provided.

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Enhancing wheat-flour safety by detecting and controlling red flour beetle Tribolium castaneum Herbst (Coleoptera: Tenebrionidae)

Gao

Hamadou

et al. 2022

J Consum Prot Food Saf

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DNA barcoding, species-specific PCR for the identification of three stored-product pest species of genus Palorus (Coleoptera: Tenebrionidae)

Cited by 7 publications

References 21 publications

Two new species of Erythroneurini (Hemiptera, Cicadellidae, Typhlocybinae) from southern China based on morphology and complete mitogenomes

Two new species of Erythroneurini (Hemiptera, Cicadellidae, Typhlocybinae) from southern China based on morphology and complete mitogenomes

Revision of the Conwentzia Enderlein, 1905 (Neuroptera, Coniopterygidae) of China, combining morphological and molecular characters

Enhancing wheat-flour safety by detecting and controlling red flour beetle Tribolium castaneum Herbst (Coleoptera: Tenebrionidae)

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