DNA Barcoding the Medusozoa using mtCOI

First report of the planktonic copepod Oithona davisae in the northern Wadden Sea (North Sea): Evidence for recent invasion?

“…DNA of the Oithona species occurring in the North Sea was extracted using the QIAGEN DNeasy Mini kit. The marker gene large-subunit (28S) rRNA was amplified and sequenced using the primer set 28SF1 and 28SR1 (Ortman 2008;Cepeda et al 2012). This gene has been successfully applied to differentiate between Oithona species (Cepeda et al 2012).…”

Description of the eudoxid stages ofLensia havockandLensia leloupi(Cnidaria: Siphonophora: Calycophorae), with a review of all knownLensiaeudoxid bracts

“…The sub-group composed of L. leloupi and L. panikkari can be differentiated from the other groups by the presence of 5 straight ridges on the anterior nectophore of the polygastric stage, all complete in their apical part, but the laterals ending some distance anterior of ostial level. The lack of support at the deeper nodes within this first Lensia clade may be due to the lack of representative 5-ridged Lensia species, such as L. challengeri or L. hotspur, the latter being a sister-taxon to L. conoidea in a study of the mitochondrial COI gene (Ortman et al, 2010).…”

“…data) of Japan. Additionally, genetic studies of the mitochondrial 16S and CO1 (Ortman et al, 2010) genes uncovered the presence of several genetically distinct groups within L. multicristata, which may reflect the presence of cryptic species. It is also interesting to note that while the large majority of L. multicristata anterior nectophores collected off south-eastern Japan resembled, in ridge pattern and somatocyst shape, the 'Lensia multicristoides'-type described by Zhang & Lin (1988, Fig.…”

Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method

“…As an alternative to morphological identification, molecular techniques have been used to identify the medusozoa (Bayaha & Graham, 2009;Bucklin et al, 2011;Daryanabard & Dawson, 2008;Ki et al, 2008Ki et al, , 2010Laakmann & Holst, 2014;Ortman et al 2010). In these studies, mitochondrial cytochrome c oxidase subunit I (COI) gene was widely used as the DNA barcode for marine Metazoa and Medusozoa (Bucklin et al, 2011;Daryanabard & Dawson, 2008;Ki et al, 2008;Laakmann & Holst, 2014;Ortman et al 2010).…”

“…In these studies, mitochondrial cytochrome c oxidase subunit I (COI) gene was widely used as the DNA barcode for marine Metazoa and Medusozoa (Bucklin et al, 2011;Daryanabard & Dawson, 2008;Ki et al, 2008;Laakmann & Holst, 2014;Ortman et al 2010). Various detection methods using genetic markers have been developed to detect jellyfish, including DNA sequencing (Daryanabard & Dawson, 2008;Ki et al, 2008;Laakmann & Holst, 2014), real-time PCR (Bayaha & Graham, 2009) and DNA microarray (Ki et al, 2010).…”