DNA binding fluorescent proteins as single-molecule probes

Jin, Xuelin; Hapsari, Natalia Diyah; Lee, Seonghyun; Jo, Kyubong

doi:10.1039/d0an00218f

Cited by 15 publications

(8 citation statements)

References 237 publications

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“…Various peptides and proteins can bind to DNA with natural affinity, termed DNA‐binding peptides/proteins (DBPs). [ 143,144 ] In biological systems, these fall under a broad family of chromosomal proteins and transcription factors, which bind to DNA and regulate gene expression. Given their natural affinity for DNA, DBPs are promising anchors for DNA nanowire generation via noncovalent biological interactions.…”

Section: Dna Metallization Via Metal Np Staining and Nanowire Growthmentioning

confidence: 99%

Toward Visualizing Genomic DNA Using Electron Microscopy via DNA Metallization

et al. 2023

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Figure 5. DNA-templated silver nanowire. The top left image is the electrode pattern used for silver nanowire assembly. A) Thiolated oligonucleotides complementary to the sticky ends of λ DNA are attached to the electrodes. B) λ DNA bridge connects the two gold electrodes. C) Silver ions are loaded on the DNA bridge. D) A silver nanowire is assembled by chemical reduction. E) Metallic silver aggregates on the λ DNA. Reproduced with permission. [55]

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Section: Dna Metallization Via Metal Np Staining and Nanowire Growthmentioning

confidence: 99%

Toward Visualizing Genomic DNA Using Electron Microscopy via DNA Metallization

et al. 2023

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“…The techniques described above enable researchers to quantitate dynamic gene expression events by measuring the final product of the process. In the past years, also the visualization of individual TFs and their dynamic interaction with genomes and cis-regulatory elements at the single-cell and single-molecule levels has greatly enhanced our models of eukaryotic gene activation [43,44].…”

Section: Technical Approaches To Capture Gene Expression In Vivomentioning

confidence: 99%

Capturing and Understanding the Dynamics and Heterogeneity of Gene Expression in the Living Cell

Pascual-Ahuir

Fita-Torró

Proft

2020

IJMS

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The regulation of gene expression is a fundamental process enabling cells to respond to internal and external stimuli or to execute developmental programs. Changes in gene expression are highly dynamic and depend on many intrinsic and extrinsic factors. In this review, we highlight the dynamic nature of transient gene expression changes to better understand cell physiology and development in general. We will start by comparing recent in vivo procedures to capture gene expression in real time. Intrinsic factors modulating gene expression dynamics will then be discussed, focusing on chromatin modifications. Furthermore, we will dissect how cell physiology or age impacts on dynamic gene regulation and especially discuss molecular insights into acquired transcriptional memory. Finally, this review will give an update on the mechanisms of heterogeneous gene expression among genetically identical individual cells. We will mainly focus on state-of-the-art developments in the yeast model but also cover higher eukaryotic systems.

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“…Fluorescent proteins (FP) have been essential molecular reporters for microscopic visualization in biochemical and cellular applications since the first application in 1994 [1]. FPs have been applied for monitoring gene expression, protein localization, protein dynamics, and protein-DNA interactions from the molecular to the cellular level [2]. FPs have distinct advantages as they are easy to use and inexpensive.…”

Section: Introductionmentioning

confidence: 99%