DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test

Brozović, Gordana; Oršolić, Nada; Rozgaj, Ružica; Kašuba, Vilena; Knežević, Fabijan; Knežević, Anica Horvat; Benković, Vesna; Lisičić, Duje; Borojević, Nikola; Đikić, Domagoj

doi:10.1007/bf03195714

Cited by 19 publications

(27 citation statements)

References 25 publications

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“…These studies have used different animal models and species, different times of exposure and routes, different doses and different time points besides a variety of other possible confounding factors such as temperature, hemodynamic data, and air flow rate, making the comparison among the reports very difficult (31). Furthermore, our previous study evaluated genetic damage in leukocytes, in the liver, kidney, and brain cells in vivo (10,32) but none of the observed tissues revealed signs of repair until 24 h after the exposure to sevoflurane (32).…”

Section: Discussionmentioning

confidence: 99%

Sevoflurane and isoflurane genotoxicity in kidney cells of mice

Brozović

Oršolić

Rozgaj

et al. 2017

Archives of Industrial Hygiene and Toxicology

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The aim of this study was to evaluate the DNA damage and repair in kidney cells of Swiss albino mice after repeated exposure to sevoflurane and isoflurane and compare their detrimental effects. We used the alkaline comet assay to establish the genetic damage and measured three parameters: tail length, tail moment, and tail intensity of comets. These parameters were measured immediately after exposure to the above mentioned inhalation anaesthetics, two hours, six hours, and 24 hours later and were compared with the control group. Mean values of all three parameters were significantly higher in experimental groups compared to the control group. DNA damage in kidney cells of mice exposed to sevoflurane increased continuously before it reached its peak 24 hours after exposure. Isoflurane induced the highest DNA damage two hours after exposure. Levels of DNA damage recorded 24 h after cessation of exposure to both tested compounds suggest that sevoflurane was slightly more genotoxic than isoflurane to kidney cells of mice. According to these results, the currently used volatile anaesthetics sevoflurane and isoflurane are able to damage DNA in kidney cells of mice. Such findings suggest a possibility for similar outcomes in humans and that fact must be taken into account in everyday clinical practice.

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Section: Discussionmentioning

confidence: 99%

Sevoflurane and isoflurane genotoxicity in kidney cells of mice

Brozović

Oršolić

Rozgaj

et al. 2017

Archives of Industrial Hygiene and Toxicology

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“…Because of its sweet smell, SVF is used for anesthesia induction in both adults and children. Conflicting data have been presented concerning the genotoxic and mutagenic potential of SVF in vitro, in animals and in patients [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

DNA damage in patients who underwent minimally invasive surgery under inhalation or intravenous anesthesia

Braz

Barbosa

et al. 2011

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Recent studies have demonstrated the genotoxicity of anesthetics in patients who have undergone surgery and in personnel who are occupationally exposed to anesthetics. However, these findings are controversial. Herein, we used the comet assay (single-cell gel electrophoresis) to investigate the genotoxic effects of two volatile compounds [isoflurane (ISF) and sevoflurane (SVF)] that are used in inhalation anesthesia, and of one intravenous (iv) anesthetic compound [propofol (PF)]. The groups consisted of 45 patients who underwent minimally invasive surgery that lasted at least 2h. Patients were classified as physical status I using the criteria of the American Society of Anesthesiologists (ASA) and were randomly allocated to receive ISF, SVF or PF anesthesia. Venous blood samples were collected at three time points as follows: before the premedication and the induction of anesthesia (T(0)); 2h after the beginning of anesthesia (T(1)); and on the day following surgery (T(2)). DNA damage (strand breaks and alkali-labile sites) was evaluated in peripheral blood lymphocytes. For each patient, one hundred nucleoids were analyzed per time point using a semi-automated image system. Patients did not differ with respect to their demographic characteristics, the duration of surgery, or the total doses of intraoperative drugs. The amount of DNA damage was not different among the three groups before anesthesia (T(0)). No statistically significant (p>0.05) increase in DNA damage was detected during (T(1)) or after anesthesia (T(2)) using three different protocols (ISF, SVF or PF). In conclusion, general anesthesia with inhaled ISF and SVF or iv PF did not induce DNA strand breaks or alkali-labile sites in peripheral lymphocytes. Therefore, our results show that the genotoxic risk of these anesthetics, for healthy patients undergoing minimally invasive otorhinological surgery, is low or even absent.

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“…We studied the activity of FS-1 in two widely used short-term genotoxicty assays, i.e., in the comet and MN assays in rats and mice bone marrow cells recommended for testing of new synthesized compounds which can be used as pharmaceuticals (15,16). Combining these two assays with differences in sensitivity, endpoints measured and the type of data generated significantly improves upon the current standard capabilities for detecting genotoxicity without requiring additional animals (17). The results of the MN experiments are presented in Tables 1 and 2.…”

Section: Resultsmentioning

confidence: 99%

Investigations of genotoxic activity of the antimicrobial/antiviral agent FS-1 in rodents by means of the micronucleus and the comet assays

Nersesyan¹,

Il’in²,

Kulmanov³

et al. 2011

Arch Oncol

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Background: FS-1 is a complex of iodine and synthesized polysaccharides and it is very effective against a number of microbe and virus strains. The aim of the study was to evaluate possible genotoxic properties of FS-1. Methods: The compound was studied in rat and mouse bone marrow micronucleus (MN) assay and the comet assay in murine peripheral blood leukocytes, hepatocytes, and kidney cells. Two treatment protocols were applied, namely acute and subacute ones. In the first protocol, the compound was administered orally once and in subacute treatment two times, 24 h apart. The animals were sacrificed 24 h after the last treatment, and appropriate cells were used to assess DNA damage and MN induction. Results: In none of the tests (MN and comet assays) significant increase compared with respective negative controls was observed. Conclusion: The fact that the compound neither induces DNA damage in various organs of mice nor is effective in the induction of MN in bone marrow cells of rats and mice is important for future genotoxicity studies of FS-1, which can be used in clinical medicine after additional testing of safety for humans

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DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test

Cited by 19 publications

References 25 publications

Sevoflurane and isoflurane genotoxicity in kidney cells of mice

Sevoflurane and isoflurane genotoxicity in kidney cells of mice

DNA damage in patients who underwent minimally invasive surgery under inhalation or intravenous anesthesia

Investigations of genotoxic activity of the antimicrobial/antiviral agent FS-1 in rodents by means of the micronucleus and the comet assays

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